公开(公告)号：US06777537B1

公开(公告)日：2004-08-17

申请号：US09618304

申请日：2000-07-18

申请人： Philip Stashenko ,  Yi-Ping Li

发明人： Philip Stashenko ,  Yi-Ping Li

IPC分类号： C07K14435

CPC分类号： C07K14/705

摘要： Novel gene encoding a human 116-kDa osteoclast proton pump subunit and the protein encoded thereby. Methods of treatment of bone mass disorders utilizing said gene, and antibodies that bind to the protein encoded by said gene.

摘要翻译： 编码人116kDa破骨细胞质子泵亚基的新基因和由此编码的蛋白质。 使用所述基因治疗骨质量障碍的方法和结合所述基因编码的蛋白质的抗体。

公开(公告)号：US06504010B1

公开(公告)日：2003-01-07

申请号：US09702705

申请日：2000-10-30

申请人： Tongtong Wang ,  Chaitanya S. Bangur ,  Michael J. Lodes ,  Gary R. Fanger ,  Thomas S. Vedvick ,  Darrick Carter ,  Marc W. Retter ,  Jane Mannion ,  Liqun Fan

发明人： Tongtong Wang ,  Chaitanya S. Bangur ,  Michael J. Lodes ,  Gary R. Fanger ,  Thomas S. Vedvick ,  Darrick Carter ,  Marc W. Retter ,  Jane Mannion ,  Liqun Fan

IPC分类号： C07K14435

CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K2039/5154 ,  A61K2039/5156 ,  C07K2319/00 ,  C12Q1/6886 ,  C12Q2600/158

摘要： Compositions and methods for the therapy and diagnosis of cancer, particularly lung cancer, are disclosed. Illustrative compositions comprise one or more lung tumor polypeptides, immunogenic portions thereof, polynucleotides that encode such polypeptides, antigen presenting cell that expresses such polypeptides, and T cells that are specific for cells expressing such polypeptides. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases, particularly lung cancer.

公开(公告)号：US06489441B1

公开(公告)日：2002-12-03

申请号：US08522726

申请日：1995-09-01

申请人： Ronald M. Evans ,  J. Don Chen

发明人： Ronald M. Evans ,  J. Don Chen

IPC分类号： C07K14435

CPC分类号： C07K14/4703 ,  G01N33/74 ,  G01N2500/00

摘要： In accordance with the present invention, there are provided novel receptor interacting factors, referred to herein as “SMRT”, i.e., a silencing mediator (co-repressor) for retinoic acid receptor (RAR) and thyroid hormone receptor (TR). SMRT is a novel protein whose association with RAR and TR both in solution and on DNA response elements is destabilized by ligand. The interaction of SMRT with mutant receptors correlates with the transcriptional silencing activities of receptors. In vivo, SMRT functions as a potent co-repressor. A GAL4 DNA binding domain (DBD) fusion of SMRT behaves as a frank repressor of a GAL4-dependent reporter. Together, these data identify a novel class of cofactor which is believed to represent an important mediator of hormone action.

摘要翻译： 根据本发明，提供了新颖的受体相互作用因子，本文称为“SMRT”，即视黄酸受体（RAR）和甲状腺激素受体（TR）的沉默介质（共抑制因子）。 SMRT是一种新的蛋白质，其与溶液中的RAR和TR在DNA响应元件上的配体不稳定。 SMRT与突变体受体的相互作用与受体的转录沉默活性相关。 在体内，SMRT作为一种有效的共抑制剂。 SMRT的GAL4 DNA结合结构域（DBD）融合作为GAL4依赖性记录的坦率阻遏物。 在一起，这些数据确定了一类新的辅因子，被认为代表激素作用的重要介质。

公开(公告)号：US06316604B1

公开(公告)日：2001-11-13

申请号：US08463959

申请日：1995-06-05

申请人： Douglas T. Fearon ,  Lloyd B. Klickstein ,  Winnie W. Wong ,  Gerald R. Carson ,  Michael F. Concino ,  Stephen H. Ip ,  Savvas C. Makrides ,  Henry C. Marsh, Jr.

发明人： Douglas T. Fearon ,  Lloyd B. Klickstein ,  Winnie W. Wong ,  Gerald R. Carson ,  Michael F. Concino ,  Stephen H. Ip ,  Savvas C. Makrides ,  Henry C. Marsh, Jr.

IPC分类号： C07K14435

CPC分类号： C07K14/70596 ,  A61K38/00 ,  Y02A50/473

摘要： The present invention relates to the C3b/C4b receptor (CR1) gene and its encoded protein. The invention also relates to CR1 nucleic acid sequences and fragments thereof comprising 70 nucleotides and their encoded peptides or proteins comprising 24 amino acids. The invention further provides for the expression of the CR1 protein and fragments thereof. The genes and proteins of the invention have uses in diagnosis and therapy of disorders involving complement activity, and various immune system or inflammatory disorders. In specific embodiments of the present invention detailed in the examples sections infra, the cloning, nucleotide sequence, and deduced amino acid sequence of a full-length CR1 cDNA and fragments thereof are described. The expression of the CR1 protein and fragments thereof is also described. Also described is the expression of a secreted CR1 molecule lacking a transmembrane region. The secreted CR1 molecule is shown to be useful in reducing damage caused by inflammation and in reducing myocardial infarct size and preventing reperfusion injury.

摘要翻译： 本发明涉及C3b / C4b受体（CR1）基因及其编码蛋白。 本发明还涉及包含70个核苷酸的CR1核酸序列及其片段及其编码的肽或包含24个氨基酸的蛋白质。 本发明进一步提供CR1蛋白及其片段的表达。 本发明的基因和蛋白质在诊断和治疗涉及补体活性的疾病以及各种免疫系统或炎性疾病中具有应用。 在下文实施例部分详述的本发明的具体实施方案中，描述了全长CR1 cDNA及其片段的克隆，核苷酸序列和推导的氨基酸序列。 还描述了CR1蛋白及其片段的表达。 还描述了缺乏跨膜区的分泌型CR1分子的表达。 分泌的CR1分子显示可用于减少由炎症引起的损伤和降低心肌梗死面积并防止再灌注损伤。

公开(公告)号：US06277592B1

公开(公告)日：2001-08-21

申请号：US08692922

申请日：1996-07-31

申请人： Christopher A. Bidwell ,  Michael E. Spurlock

发明人： Christopher A. Bidwell ,  Michael E. Spurlock

IPC分类号： C07K14435

CPC分类号： C07K14/5759 ,  A61K38/00

摘要： A porcine adipocyte-specific polypeptide, termed leptin, is expressed in the fat tissue of pigs. Expression may be altered in over fat pigs, or expression may be in the form of a protein of lesser biological activity relative to that of leaner pigs. The porcine adipocyte polypeptide, DNA and RNA molecules coding therefor, methods for its preparation, and antibodies specific for the polypeptide are disclosed. Methods for determining the susceptibility of a pig to fat deposition are based on measuring the levels of the porcine adipocyte polypeptide in a biological fluid or tissue extract or by measuring mRNA encoding the porcine adipocyte polypeptide in cells of the subject. Methods of evaluating an agent related to the deposition of fat in swine comprise contacting the agent with an adipocyte in vitro and measuring the amount of the porcine adipocyte polypeptide or mRNA that is produced by the adipocyte. Methods of limiting fat deposition include administering porcine leptin or porcine leptin DNA, and methods of regulating intake include administering porcine leptin, porcine leptin DNA, or an antibody directed against porcine leptin.

摘要翻译： 称为瘦素的猪脂肪细胞特异性多肽在猪的脂肪组织中表达。 在肥胖的猪中可能会改变表达，或者表达可能是相对于瘦猪的生物活性较小的蛋白质的形式。 公开了猪脂肪细胞多肽，其编码的DNA和RNA分子，其制备方法和对多肽特异的抗体。 用于测定猪对脂肪沉积的敏感性的方法是基于测量生物流体或组织提取物中猪脂肪细胞多肽的水平或通过测量在受试者的细胞中编码猪脂肪细胞多肽的mRNA。 评估与猪中脂肪沉积有关的试剂的方法包括在体外使试剂与脂肪细胞接触并测量由脂肪细胞产生的猪脂肪细胞多肽或mRNA的量。 限制脂肪沉积的方法包括施用猪瘦素或猪瘦蛋白DNA，调节摄入量的方法包括施用猪瘦蛋白，猪瘦素DNA或针对猪瘦蛋白的抗体。

公开(公告)号：US06184349B2

公开(公告)日：2001-02-06

申请号：US08843417

申请日：1997-04-15

申请人： Ronald Herman ,  Stephen Gregory Delgado ,  Linda Marie Fish ,  Lakshmi Sangameswaran ,  Douglas Kenneth Rabert

发明人： Ronald Herman ,  Stephen Gregory Delgado ,  Linda Marie Fish ,  Lakshmi Sangameswaran ,  Douglas Kenneth Rabert

IPC分类号： C07K14435

CPC分类号： C07K14/705

摘要： The present invention relates to a voltage-gated sodium channel present in peripheral nerve tissue that is tetrodotoxin-resistant. One aspect of the present invention is purified and isolated DNA encoding this sodium channel. Another aspect of the present invention is the recombinant protein expressed by this DNA, expression vectors comprising the DNA sequence, and host cells transformed with these expression vectors. Another aspect of this invention is the use of this voltage-gated, tetrodotoxin-resistant sodium channel as a therapeutic target for compounds to treat disorders of the peripheral nervous system.

摘要翻译： 本发明涉及存在于患有河豚毒素的周围神经组织中的电压门控钠通道。 本发明的一个方面是纯化和分离的编码该钠通道的DNA。 本发明的另一方面是由该DNA表达的重组蛋白，包含DNA序列的表达载体和用这些表达载体转化的宿主细胞。 本发明的另一方面是使用该电压门控的河豚毒素钠通道作为化合物治疗周围神经系统疾病的治疗靶标。

公开(公告)号：US06753411B2

公开(公告)日：2004-06-22

申请号：US09816248

申请日：2001-03-26

申请人： Peter Baumann ,  Thomas R. Cech

发明人： Peter Baumann ,  Thomas R. Cech

IPC分类号： C07K14435

CPC分类号： C12N9/1241 ,  A61K38/00 ,  A61K48/00 ,  C07K14/39 ,  C07K14/4703

摘要： A protein identified in humans and Schizosaccharomyces pombe, Pot1p, binds single-stranded telomeric DNA and both stabilizes chromosome ends and regulates telomerase activity. Compounds that stabilize or disrupt the Pot1p-DNA interaction will be useful in regulating the telomere length of a cell. Because telomere length is involved in the regulation of cellular life-span, the life-span of useful cell populations may be prolonged or undesirable cells may be caused to cease proliferation. The identification of a Pot1 protein and its encoding DNA provides methods of screening useful compounds or diagnosing illnesses that involve altered expression or structure of a Pot1 protein or gene.

摘要翻译： 在人类和粟酒裂殖酵母中鉴定的蛋白质Pot1p结合单链端粒DNA，并稳定染色体末端并调节端粒酶活性。 稳定或破坏Pot1p-DNA相互作用的化合物可用于调节细胞的端粒长度。 由于端粒长度参与细胞寿命的调节，因此可能延长有用细胞群体的寿命或者可能导致不希望的细胞停止增殖。 Pot1蛋白及其编码DNA的鉴定提供了筛选有用化合物或诊断涉及改变Pot1蛋白或基因表达或结构的疾病的方法。

公开(公告)号：US06660841B1

公开(公告)日：2003-12-09

申请号：US09522689

申请日：2000-03-10

申请人： Paolo Sassone-Corsi

发明人： Paolo Sassone-Corsi

IPC分类号： C07K14435

CPC分类号： C07K14/4705 ,  C07K2319/00

摘要： The present invention deals with a protein activation of CREM in Testis named ACT, the cDNA encoding ACT and a method to select compounds which are able to interact with ACT or its site of interaction on CREM to block or stimulate the CREM transcriptional activity in Testis. Such compounds may be useful to control male fertility.

摘要翻译： 本发明涉及睾丸中CREM的蛋白激活，其命名为ACT，编码ACT的cDNA和选择能与ACT或其与CREM相互作用的位点相互作用以阻断或刺激睾丸中的CREM转录活性的化合物的方法。 这些化合物可用于控制雄性生育力。

公开(公告)号：US06525177B2

公开(公告)日：2003-02-25

申请号：US09751798

申请日：2000-12-29

申请人： Elisabeth Stockert ,  Elke Jäger ,  Yao-tseng Chen ,  Matthew Scanlan ,  Knuth Alexander ,  Lloyd J. Old

发明人： Elisabeth Stockert ,  Elke Jäger ,  Yao-tseng Chen ,  Matthew Scanlan ,  Knuth Alexander ,  Lloyd J. Old

IPC分类号： C07K14435

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00 ,  C07K14/47 ,  C07K16/18

摘要： The invention relates to antibodies which bind to the cancer associated antigen NY-ESO-1. Both polyclonal and monoclonal antibodies are part of the invention, as are chimeric forms of the antibodies, and binding portions of antibodies. Uses of these antibodies are described. Also described are truncated, recombinant forms of the cancer associated antigen.

公开(公告)号：US06518401B2

公开(公告)日：2003-02-11

申请号：US09799118

申请日：2001-03-06

申请人： Tae Ho Lee ,  Hans-Georg Wisniewski ,  Jan Vilcek

发明人： Tae Ho Lee ,  Hans-Georg Wisniewski ,  Jan Vilcek

IPC分类号： C07K14435

CPC分类号： C07K14/4718

摘要： TSG protein and functional derivatives thereof, DNA coding therefor, expression vehicles, such as plasmids, and host cells transformed or transfected with the DNA molecule, and methods for producing the protein and the DNA are provided, as well as antibodies specific for the TSG-6 protein; a method for detecting the presence of TSG-6 protein in a biological sample; a method for detecting the presence of nucleic acid encoding a normal or mutant TSG-6 protein; a method for measuring induction of expression of TSG-6 in a cell using either nucleic acid hybridization or immunoassay; a method for identifying a compound capable of inducing the expression of TSG-6 in a cell; and a method for measuring the ability of a cell to respond to TNF.

摘要翻译： 提供TSG蛋白及其功能性衍生物，编码DNA，表达载体如质粒，用DNA分子转化或转染的宿主细胞，以及产生蛋白质和DNA的方法，以及TSG- 6蛋白; 用于检测生物样品中TSG-6蛋白的存在的方法; 检测编码正常或突变型TSG-6蛋白的核酸的存在的方法; 使用核酸杂交或免疫测定来测量细胞中TSG-6的表达诱导的方法; 用于鉴定能够诱导细胞中TSG-6表达的化合物的方法; 以及测定细胞对TNF反应能力的方法。