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    DNA sequences encoding insulin precursors and methods of production
    101.
    发明授权
    DNA sequences encoding insulin precursors and methods of production 失效
    编码胰岛素前体的DNA序列和生产方法

    公开(公告)号：US5324641A

    公开(公告)日：1994-06-28

    申请号：US952696

    申请日：1992-09-23

    申请人： Ib Jonassen ,  Ib G. Clausen ,  Ejner B. Jensen ,  Allan Svendsen

    发明人： Ib Jonassen ,  Ib G. Clausen ,  Ejner B. Jensen ,  Allan Svendsen

    IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/575 ,  C07K14/62 ,  C12N15/17 ,  C12P21/02 ,  C12R1/865 ,  C12N15/00 ,  C12N1/08 ,  C12P21/06

    CPC分类号： C07K14/62 ,  A61K38/00

    摘要： DNA molecule and process for producing insulin precursors having the formula B(1-29) -H.sub.1 --X.sub.2 --Y.sub.2 --Y.sub.1 --A(1-21), wherein B(1-29) are the 29 first amino acid residues of the B chain of human insulin starting from the N-terminus, A(1-21) are the 21 amino acid residues of the A chain of human insulin, X.sub.1 represents a peptide bond or one naturally-occurring alpha-amino acid acid residues, X.sub.2 represents Glu or Asp, and Y.sub.1 and Y.sub.2 each represent Lys or Arg, the positions A6 and A11, A7 and B7 and A20 and B19, respectively, are connected through sulphur bridges, and, if desired, one or more of the glutamic acid residues in positions A4, A17, B13 and B21 are substituted by another naturally-occurring alpha-amino acid residue having an uncharged side chain, are provided. The insulin precursors are prepared by culturing a yeast strain transformed with a replicable plasmid comprising a DNA sequence encoding an insulin precursor of the above formula in a suitable medium and isolating the insulin precursor thus formed from the culture medium. The insulin precursor can be converted into human insulin or insulin analogues by enzymatic treatment in a manner known per se.

    摘要翻译： DNA分子和具有式B（1-29）-H1-X2-Y2-Y1-A（1-21）的胰岛素前体的制备方法，其中B（1-29）是B的第一个氨基酸残基 A（1-21）是人胰岛素A链的21个氨基酸残基，X1表示肽键或一个天然存在的α-氨基酸酸残基，X2表示 Glu或Asp，Y1和Y2各自表示Lys或Arg，位置A6和A11，A7和B7和A20和B19分别通过硫桥连接，如果需要，一个或多个谷氨酸残基在 位置A4，A17，B13和B21被另一个具有不带电侧链的天然存在的α-氨基酸残基所取代。 通过在合适的培养基中培养用包含编码上式胰岛素前体的DNA序列的可复制质粒转化的酵母菌株并分离由培养基形成的胰岛素前体来制备胰岛素前体。 胰岛素前体可以通过本身已知的方式通过酶处理转化为人胰岛素或胰岛素类似物。

    Subtilases
    107.
    发明申请
    Subtilases 有权
    分散液

    公开(公告)号：US20120088288A1

    公开(公告)日：2012-04-12

    申请号：US13304880

    申请日：2011-11-28

    申请人： Allan Svendsen ,  Henriette Draborg

    发明人： Allan Svendsen ,  Henriette Draborg

    IPC分类号： C12N9/52 ,  C12N15/63 ,  C12N1/21 ,  C12N15/57

    CPC分类号： C12N9/54 ,  C11D3/386 ,  C12Y304/21062

    摘要： The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.

    摘要翻译： 本发明涉及用于产生亲本TY145脱落酶和亲本BPN'脱羧酶的变体的方法，并且与亲本TY145 / BPN'脱羧酶相比具有改变的性质的TY145和BPN'变体的方法。

    α-amylase mutants
    109.
    发明授权
    α-amylase mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US07993897B2

    公开(公告)日：2011-08-09

    申请号：US12100506

    申请日：2008-04-10

    申请人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    发明人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    IPC分类号： C12N9/30 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C11D3/386 ,  C12N9/2417

    摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.

    摘要翻译： 本发明涉及构建母体类戊酰样α-淀粉酶的变体的方法，该变体与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类戊酰样α-淀粉酶结构的至少一个结构部分，该氨基酸残基或结构部分被认为与改变 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变性质，以及任选地，iii）相对于所讨论的性质测试所得到的类戊酰样α-淀粉酶变体。

    Amylase Variants
    110.
    发明申请
    Amylase Variants 审中-公开
    淀粉酶变体

    公开(公告)号：US20110177990A1

    公开(公告)日：2011-07-21

    申请号：US13023029

    申请日：2011-02-08

    申请人： Henrik Bisgârd-Frantzen ,  Allan Svendsen ,  Torben Vedel Borchert

    发明人： Henrik Bisgârd-Frantzen ,  Allan Svendsen ,  Torben Vedel Borchert

    IPC分类号： C11D3/386 ,  C12N9/28 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15

    CPC分类号： C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/2417 ,  C12Y302/01001 ,  D06L1/14

    摘要： The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

    摘要翻译： 本发明涉及母体α-淀粉酶的变体，其亲本α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶表现出以下特性中的至少一个：增加的热稳定性; 提高氧化稳定性; 并减少Ca2 +依赖; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。