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147 条记录 (耗时 0.1 秒)

    Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
    103.
    发明授权
    Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants 有权
    用于制备蛋白酶抗性人生长激素糖基化突变体的组合物和方法

    公开(公告)号：US09187546B2

    公开(公告)日：2015-11-17

    申请号：US11910958

    申请日：2006-04-10

    申请人： Shawn DeFrees

    发明人： Shawn DeFrees

    IPC分类号： C07K14/61 ,  A61K38/27 ,  A61P5/06 ,  C12N15/09 ,  A61K38/00

    CPC分类号： C07K14/61 ,  A61K38/00 ,  A61K38/27 ,  C12N15/09

    摘要： The present invention relates to protease resistant mutants of human growth hormone, which contain newly introduced proteolysis resistant mutations and N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.

    摘要翻译： 本发明涉及人生长激素的蛋白酶抗性突变体，其包含新引入的蛋白水解抗性突变和N-连接或O-连接的糖基化位点，使得这些重组产生的多肽具有与天然的糖基化模式明显不同的糖基化模式 发生人类生长激素。 还公开了突变体的多核苷酸编码序列，包含编码序列的表达盒，表达突变体的细胞，以及用于产生突变体的方法。 还公开了包含突变体和使用突变体的方法的药物组合物。

    Methods of refolding mammalian glycosyltransferases
    105.
    发明授权
    Methods of refolding mammalian glycosyltransferases 有权
    重复哺乳动物糖基转移酶的方法

    公开(公告)号：US08822191B2

    公开(公告)日：2014-09-02

    申请号：US10587769

    申请日：2005-02-04

    申请人： Sami Saribas ,  David Hakes ,  Scott Willett ,  Karl F. Johnson ,  Daniel James Bezila ,  Shawn DeFrees

    发明人： Sami Saribas ,  David Hakes ,  Scott Willett ,  Karl F. Johnson ,  Daniel James Bezila ,  Shawn DeFrees

    IPC分类号： C12P21/06 ,  C12N9/10

    CPC分类号： C12N9/1048

    摘要： The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.

    摘要翻译： 本发明提供了在细菌细胞中产生的哺乳动物糖基转移酶重折叠的方法，以及使用这种重折叠的糖基转移酶（包括具有增强的重折叠能力的糖基转移酶突变体）的方法。 本发明还提供了在单个容器中重折叠多于一种糖基转移酶的方法，使用这种重折叠的糖基转移酶的方法和包含重折叠的糖基转移酶的反应混合物。

    Glycopegylated erythropoietin
    106.
    发明申请
    Glycopegylated erythropoietin 失效
    糖基化促红细胞生成素

    公开(公告)号：US20100210507A9

    公开(公告)日：2010-08-19

    申请号：US11144223

    申请日：2005-06-02

    申请人： Shawn DeFrees ,  Robert Bayer ,  David Zopf

    发明人： Shawn DeFrees ,  Robert Bayer ,  David Zopf

    IPC分类号： A61K38/18 ,  C07K14/505

    CPC分类号： A61K38/1816

    摘要： The present invention provides conjugates between erythropoietin and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates.

    摘要翻译： 本发明提供红细胞生成素和PEG部分之间的缀合物。 缀合物通过插入和共价连接到肽和修饰基团之间的完整糖基连接基团连接。 通过糖基转移酶的作用，由糖基化肽形成缀合物。 糖基转移酶将修饰的糖部分连接到肽上的糖基残基上。 还提供了制备缀合物的方法，用缀合物治疗各种疾病状况的方法，以及包括缀合物的药物制剂。

    PROCESS FOR THE PRODUCTION OF NUCLEOTIDE SUGARS
    107.
    发明申请
    PROCESS FOR THE PRODUCTION OF NUCLEOTIDE SUGARS 有权
    生产核糖的工艺

    公开(公告)号：US20100174059A1

    公开(公告)日：2010-07-08

    申请号：US12663748

    申请日：2008-06-12

    申请人： Shawn DeFrees ,  Caryn Bowe

    发明人： Shawn DeFrees ,  Caryn Bowe

    IPC分类号： C07H19/10 ,  C07H1/06

    CPC分类号： C07H19/10

    摘要： The current invention provides methods (e.g., large-scale processes) for the production of nucleotide sugars, which are modified with a polymeric modifying group, such as poly(alkylene oxide) moieties (e.g., poly(ethylene glycol) or poly(propylene glycol)) moieties. A typical process of the invention includes anion exchange chromatography followed by an ultrafiltration procedure, such as tangential flow filtration. The process of the invention provides modified nucleotide sugars in unexpectedly high purity and high overall yields.

    摘要翻译： 本发明提供用于生产核苷酸糖的方法（例如，大规模方法），其用聚合修饰基团修饰，例如聚（环氧烷）部分（例如聚（乙二醇）或聚（丙二醇） ）部分。 本发明的典型方法包括阴离子交换层析，然后进行超滤方法，例如切向流过滤。 本发明的方法提供了出乎意料的高纯度和高总产率的修饰的核苷酸糖。

    COMPOSITIONS AND METHODS FOR THE PREPARATION OF PROTEASE RESISTANT HUMAN GROWTH HORMONE GLYCOSYLATION MUTANTS
    110.
    发明申请
    COMPOSITIONS AND METHODS FOR THE PREPARATION OF PROTEASE RESISTANT HUMAN GROWTH HORMONE GLYCOSYLATION MUTANTS 有权
    用于制备耐药性人类生长激素谷氨酸化突变体的组合物和方法

    公开(公告)号：US20090124544A1

    公开(公告)日：2009-05-14

    申请号：US11910958

    申请日：2006-04-10

    申请人： Shawn DeFrees

    发明人： Shawn DeFrees

    IPC分类号： A61K38/22 ,  C07H21/04 ,  C12N15/64 ,  C12N15/09 ,  A61P5/06 ,  C12N1/21 ,  C07K14/61

    CPC分类号： C07K14/61 ,  A61K38/00 ,  A61K38/27 ,  C12N15/09

    摘要： The present invention relates to protease resistant mutants of human growth hormone, which contain newly introduced proteolysis resistant mutations and N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.

    摘要翻译： 本发明涉及人生长激素的蛋白酶抗性突变体，其包含新引入的蛋白水解抗性突变和N-连接或O-连接的糖基化位点，使得这些重组产生的多肽具有与天然的糖基化模式明显不同的糖基化模式 发生人类生长激素。 还公开了突变体的多核苷酸编码序列，包含编码序列的表达盒，表达突变体的细胞，以及用于产生突变体的方法。 还公开了包含突变体和使用突变体的方法的药物组合物。