公开(公告)号：US20210214730A1

公开(公告)日：2021-07-15

申请号：US17058989

申请日：2019-05-24

Applicant: Wake Forest University Health Sciences

Inventor： William H. Gmeiner

IPC: C12N15/113 ,  C07H21/04 ,  A61K9/00 ,  A61K45/06 ,  A61P35/04

Abstract: Novel cytotoxic fluoropyrimidine polymers for treating cancer (e.g., colorectal cancer (CRC)) in a subject (e.g., a subject having cancer) are nucleic acid molecules including 5-fluoro-2′deoxyuridine monophosphate (FdUMP)[10] having a polyethylene glycol (PEG) spacer appended to the 5′ terminus and a cytarabine (AraC) nucleotide appended to the 3′terminus. These novel cytotoxic fluoropyrimidine polymers may be used in compositions, kits and methods for treating advanced and high-risk cancers, including CRC.

公开(公告)号：US11028502B2

公开(公告)日：2021-06-08

申请号：US16171504

申请日：2018-10-26

Applicant: Wake Forest University Health Sciences

Inventor： Sang Jin Lee ,  James J. Yoo ,  Young Min Ju ,  Anthony Atala

IPC: D01D5/00 ,  A61L27/24 ,  A61K35/44 ,  C07K16/46 ,  A61K31/737 ,  A61K38/36 ,  A61K38/18 ,  A61K31/729 ,  A61K38/39 ,  C07K16/28 ,  C07D315/00 ,  A61K31/728 ,  D01F6/92 ,  C07K16/18 ,  D01F1/10 ,  D01F6/62 ,  D01F4/00

Abstract: The invention is directed to products and methods for preparing self-seeding vascular constructs generated as a bi-layered electrospun matrices, conjugated with EPC-specific antibodies and anti-thrombogenic agents on the inner surfaces of their lumens.

公开(公告)号：US20210162020A1

公开(公告)日：2021-06-03

申请号：US16935437

申请日：2020-07-22

Applicant: Wake Forest University Health Sciences ,  Genethon ,  Children's Medical Center Corporation

Inventor： Martin K. Childers ,  Alan H. Beggs ,  Ana Maria Buj Bello

IPC: A61K38/46 ,  A61K48/00 ,  C12N7/00 ,  C12N9/16

Abstract: The present invention provides compositions and methods for treating a myopathy. In certain embodiments, the invention provides compositions and methods for treating, improving muscle function, and prolonging survival in a subject with X-linked myotubular myopathy (XLMTM). The present invention provides a method comprising systemic administration of a composition that induces the increased expression of myotubularin in the muscle of a subject. The invention provides sustained regional and global increases in muscle function.

公开(公告)号：US11001811B2

公开(公告)日：2021-05-11

申请号：US15768100

申请日：2016-10-14

Applicant: Wake Forest University Health Sciences

Inventor： Sean V. Murphy ,  Anthony Atala

IPC: C12N5/071 ,  C12M3/00 ,  C12M3/06 ,  C12M1/00

Abstract: Provided herein are artificial lung organoids. The artificial lung organoids may include an epithelial cell layer comprising mammalian lung epithelial cells, a stromal cell layer comprising mammalian lung fibroblast cells and an endothelial cell layer comprising mammalian endothelial cells. The artificial lung organoids may optionally include a porous membrane between said epithelial cell layer and said stromal cell layer and/or between said stromal cell layer and said endothelial lung cell layer.

公开(公告)号：US10993433B2

公开(公告)日：2021-05-04

申请号：US15294154

申请日：2016-10-14

Applicant: Wake Forest University Health Sciences

Inventor： Samuel Pendergraft ,  Hooman Sadri-Ardekani ,  Anthony Atala ,  Colin Bishop

IPC: C12N5/22 ,  A01N1/02 ,  C12N5/071

Abstract: A cell composition composed of spermatogonial stem cells, Sertoli cells, Leydig cells and optionally peritubular cells, is provided, as is a culture composition, artificial testicular construct, hydrogel composition, and device containing the same. A method for using the device as a physiologically relevant in vitro model of human testicular function to screen compounds for pharmacological or toxicological activity is also provided.

公开(公告)号：US10900071B2

公开(公告)日：2021-01-26

申请号：US15758814

申请日：2016-05-12

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Adam R. Hall

IPC: C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12P19/34 ,  C07H21/04 ,  C12N15/10

Abstract: Described are methods of detecting modified nucleotide bases in a DNA sample using specific DNA glycosylases to excise target modified bases. DNA molecules are then labeled using a DNA polymerase lacking 3′→5′ exo-nuclease activity and strand displacement activity. The methods can be used to detect epigenetic changes and DNA damage. Provided are methods for diagnosing a disease or condition, determining risk of a disease or condition, identifying appropriate treatment, monitoring effectiveness of treatment, and monitoring side effects of treatment in subjects based on detection of modified bases. Also provided are methods for determining environmental exposure, or an environmental exposure time, of a biological sample containing DNA. Also provided are kits, systems, and devices for performing the described methods.

公开(公告)号：US20200347113A1

公开(公告)日：2020-11-05

申请号：US16947154

申请日：2020-07-21

Applicant: Wake Forest University Health Sciences

Inventor： Waldemar Debinski ,  Carla Lema Tome ,  Sara Ferluga ,  Poonam S. Sonawane

IPC: C07K14/705 ,  C07K14/52 ,  C07K14/54 ,  G01N33/574

Abstract: Provided herein is a construct comprising, in combination: an EphA3, EphA2 and/or EphB2 binding ligand; and at least one effector molecule. In some embodiments, the at least one effector molecule comprises a therapeutic agent, a nanoparticle, a detectable group, a lipid, or a liposome. In some embodiments, the construct is a fusion protein and/or a covalent conjugate. Further provided is a construct comprising, in combination: a ligand that binds to EphA2, EphA3 and/or EphB2; a ligand that binds to IL-13Rα2; and at least one effector molecule. Also provided are methods of use thereof for treating cancer.

公开(公告)号：US20200238024A1

公开(公告)日：2020-07-30

申请号：US16696738

申请日：2019-11-26

Applicant: Wake Forest University Health Sciences

Inventor： James J. Yoo ,  Anthony Atala ,  Kyle W. Binder ,  Mohammad Z. Albanna ,  Weixin Zhao ,  Dennis Dice ,  Tao Xu

IPC: A61M11/00 ,  A61B5/00 ,  A61B5/107 ,  A61L27/22 ,  A61L27/24 ,  A61L27/38 ,  A61L27/60 ,  C12N5/077 ,  A61M35/00

Abstract: Provided herein is a delivery system, including: (a) an optical sensor configured to detect data to create a map of a patient bodily surface; and (b) a dispenser operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the patient bodily surface based upon the data or map. Methods of forming a tissue on a patient bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing patient bodily surface data.

公开(公告)号：US20200093891A1

公开(公告)日：2020-03-26

申请号：US16496117

申请日：2018-03-29

Applicant: Wake Forest University Health Sciences

Inventor： James K. Williams

IPC: A61K38/19 ,  A61P13/12 ,  A61K9/00 ,  A61K47/42 ,  A61K38/18

Abstract: The present disclosure provides compositions and methods for treatment of a kidney disease with chemokine protein stromal cell-derived factor 1 (SDF-1), such as methods of treating a subject afflicted with or at risk of developing a kidney disease, comprising administering SDF-1 to a kidney of the subject in a treatment-effective amount.

公开(公告)号：US20200063210A1

公开(公告)日：2020-02-27

申请号：US16466663

申请日：2017-12-05

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Timothy S. Pardee ,  Lance D. Miller

IPC: C12Q1/6886 ,  G06F17/18 ,  G16H50/30 ,  G16B25/10

Abstract: Compositions and methods are disclosed for identifying patients for treatment of hematological malignancy.