公开(公告)号：US12110549B2

公开(公告)日：2024-10-08

申请号：US17822117

申请日：2022-08-24

Applicant: 10x Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20240002837A1

公开(公告)日：2024-01-04

申请号：US18367638

申请日：2023-09-13

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12N15/10 ,  C40B20/04 ,  C12Q1/6804 ,  C12Q1/6806 ,  C40B50/16 ,  C12Q1/6874 ,  C12Q1/683

CPC classification number: C12N15/1065 ,  C40B20/04 ,  C12Q1/6804 ,  C12Q1/6806 ,  C40B50/16 ,  C12Q1/6874 ,  C12Q1/683 ,  C12Q2537/143 ,  C12Q2525/191 ,  C12Q2565/629 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2537/149 ,  C12Q2535/122 ,  C12Q2563/149

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20230348897A1

公开(公告)日：2023-11-02

申请号：US18207023

申请日：2023-06-07

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12N15/10 ,  C40B50/16 ,  C40B20/04 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12N15/1065 ,  C40B50/16 ,  C40B20/04 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11703427B2

公开(公告)日：2023-07-18

申请号：US16434605

申请日：2019-06-07

Applicant: 10X GENOMICS, INC.

Inventor： Christopher Hindson ,  Andrew D. Price ,  Michael Schnall-Levin

IPC: G01N1/28 ,  G01N1/40 ,  C12N5/00

CPC classification number: G01N1/28 ,  C12N5/0006 ,  G01N1/4077 ,  C12N2537/10

Abstract: The present disclosure provides methods and systems for cell and bead processing or analysis. A method for processing a cell or bead may include subjecting a bead to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). Such a method may further include subjecting the cell or bead to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks may be formed within the cell or bead.

公开(公告)号：US20220154175A1

公开(公告)日：2022-05-19

申请号：US17517287

申请日：2021-11-02

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Christopher Hindson ,  Donald Masquelier ,  Mirna Jarosz ,  Michael Schnall-Levin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12N15/11 ,  G01N33/543 ,  C12Q1/6876 ,  C12Q1/6874

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11193121B2

公开(公告)日：2021-12-07

申请号：US16170980

申请日：2018-10-25

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Christopher Hindson ,  Donald Masquelier ,  Mirna Jarosz ,  Michael Schnall-Levin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12N15/11 ,  G01N33/543 ,  C12Q1/6876 ,  C12Q1/6874

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US10752950B2

公开(公告)日：2020-08-25

申请号：US16435417

申请日：2019-06-07

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US10752949B2

公开(公告)日：2020-08-25

申请号：US16212441

申请日：2018-12-06

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20200033237A1

公开(公告)日：2020-01-30

申请号：US16434605

申请日：2019-06-07

Applicant: 10X GENOMICS, INC.

Inventor： Christopher Hindson ,  Andrew D. Price ,  Michael Schnall-Levin

IPC: G01N1/28 ,  C12N5/00 ,  G01N1/40

Abstract: The present disclosure provides methods and systems for cell and bead processing or analysis. A method for processing a cell or bead may comprise subjecting a bead to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). Such a method may further comprise subjecting the cell or bead to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks may be formed within the cell or bead.

公开(公告)号：US20190367966A1

公开(公告)日：2019-12-05

申请号：US16375102

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC: C12Q1/6806 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6804 ,  G01N33/50 ,  C12N15/10

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.