公开(公告)号：US20170044562A1

公开(公告)日：2017-02-16

申请号：US15336906

申请日：2016-10-28

Applicant: BASF Plant Science Company GmbH

Inventor： Josef Martin Kuhn ,  Linda Patricia LOYALL ,  Malte SIEBERT ,  Elke DUWENIG

IPC: C12N15/82

CPC classification number: C12N15/8234 ,  C12N5/04 ,  C12N15/8216 ,  C12N15/8241

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing seed-specific and/or seed-preferential promoters and the production of plants with enhanced seed-specific and/or seed-preferential expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to the promoters and/or introduced into plants.

Abstract translation: 本发明涉及植物分子生物学领域，并且提供用于生产高表达种子特异性和/或种子优先启动子的方法以及产生具有增强的种子特异性和/或种子优先表达的核酸的植物，其中 核酸表达增强核酸（NEENA）在功能上与启动子连接和/或引入植物。

公开(公告)号：US20170044561A1

公开(公告)日：2017-02-16

申请号：US15336872

申请日：2016-10-28

Applicant: BASF Plant Science Company GmbH

Inventor： Josef Martin Kuhn ,  Linda Patricia Loyall ,  Malte Siebert ,  Elke Duwenig

IPC: C12N15/82

CPC classification number: C12N15/8234 ,  C12N5/04 ,  C12N15/8216 ,  C12N15/8241

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing seed-specific and/or seed-preferential promoters and the production of plants with enhanced seed-specific and/or seed-preferential expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to the promoters and/or introduced into plants.

公开(公告)号：US20160348126A1

公开(公告)日：2016-12-01

申请号：US14893713

申请日：2014-05-19

Applicant: BASF Plant Science Company GmbH

Inventor： Marieke Louwers

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N15/825 ,  C12N15/8266 ,  Y02A40/146

Abstract: Plants having one or more enhanced yield-related traits and a method for making the same are provided. It relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, a method for enhancing one or more yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a POI (Protein Of Interest) polypeptide is provided. Plants having modulated expression of a nucleic acid encoding a POI polypeptide are also provided, which plants have one or more enhanced yield-related traits compared with control plants. Hitherto unknown POI-encoding nucleic acids and constructs comprising the same useful in performing the method are also provided.

Abstract translation: 提供具有一种或多种增强的产量相关性状的植物及其制备方法。 它一般涉及分子生物学领域，并且涉及增强植物中各种经济上重要的产量相关性状的方法。 更具体地，提供了通过调节植物中编码POI（Protein Of Interest）多肽的核酸的表达来增强植物中一种或多种产量相关性状的方法。 还提供了调节表达编码POI多肽的核酸的植物，与对照植物相比，植物具有一种或多种增强的产量相关性状。 还提供了迄今为止未知的POI编码核酸和包含其在实施该方法中有用的构建体。

公开(公告)号：US20160168584A1

公开(公告)日：2016-06-16

申请号：US14902715

申请日：2014-07-03

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Joerg Bauer

IPC: C12N15/82

Abstract: The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field.For the production of desired molecules in plant cells, e.g. PUFAs, it is frequently required to express a target gene heterologous to the plant cell, or to overexpress a target gene naturally found in said plant cell.

Abstract translation: 本发明涉及转基因核酸，表达盒，载体，植物细胞，植物器官和植物。 本发明还涉及用于增加靶基因，特别是在植物细胞或植物器官中的表达或活性的方法，以及重组核酸和表达盒的用途以增加靶基因的表达或活性或用于制备 载体，植物细胞，植物器官或植物。 顺便提及，本发明涉及当可操作地连接到在这种植物细胞，植物器官或植物中起作用的启动子时，用于实现靶基因，特别是在植物细胞或植物器官中增加的表达或活性的增强子。 本发明是参照多不饱和脂肪酸（PUFA）的生产技术领域而不受本技术领域的限制。 为了在植物细胞中产生所需的分子，例如。 PUFA通常需要表达与植物细胞异源的靶基因，或过表达在所述植物细胞中天然存在的靶基因。

公开(公告)号：US09335252B2

公开(公告)日：2016-05-10

申请号：US14730531

申请日：2015-06-04

Applicant: BASF Plant Science Company GmbH

Inventor： Frederik Leyns ,  Cédrick Vandaele ,  Pierre Lejeune ,  Jeroen Baert ,  Fabio Fiorani

IPC: G01N21/64 ,  G01N21/17 ,  A01G7/00 ,  G01N21/84 ,  A01G1/00 ,  G01N33/00

CPC classification number: G01N21/17 ,  A01G7/00 ,  A01G22/00 ,  G01N21/84 ,  G01N33/0098 ,  G01N2021/1765 ,  G01N2021/8466

Abstract: A screening device (110) for screening at least one plant specimen (112) in a plurality of plant specimens (114) is disclosed. The screening device (110) comprises a detector (116) adapted for acquiring spatially resolved images (117). The screening device (110) further comprises at least one selection device (118) adapted for selecting a single plant specimen (120) or a group of plant specimens (122) from the plurality of plant specimens (114) for imaging by the detector (116). The selection device (118) comprises a deflection device (124) adapted for deflecting electromagnetic waves propagating between the plant specimens (112) and the detector (116).

公开(公告)号：US20160108419A1

公开(公告)日：2016-04-21

申请号：US14889236

申请日：2014-04-29

Applicant: BASF Plant Science Company GmbH

Inventor： Steven VANDENABEELE

IPC: C12N15/82 ,  C07K14/245

CPC classification number: C12N15/8261 ,  C07K14/245 ,  C12N15/8273 ,  Y02A40/146

Abstract: A method for enhancing various economically important yield-related traits in plants is provided. More specifically, a method for enhancing one or more yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a POI (Protein Of Interest) polypeptide is provided.

Abstract translation: 提供了一种增强植物中各种经济上重要的产量相关性状的方法。 更具体地，提供了通过调节植物中编码POI（Protein Of Interest）多肽的核酸的表达来增强植物中一种或多种产量相关性状的方法。

公开(公告)号：US20160068856A1

公开(公告)日：2016-03-10

申请号：US14772140

申请日：2014-03-07

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Holger Schultheiss ,  Ralf Flachmann ,  Tobias Mentzel

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8282 ,  C07K14/415

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales in plants and/or plant cells. This is achieved by increasing the expression of a MybTF protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding a MybTF protein.

Abstract translation: 本发明涉及一种在植物和/或植物细胞中增加对丁酸杆菌的真菌病原体的抗性的方法。 与野生型植物，野生型植物部分和/或野生型植物细胞相比，通过增加植物，植物部分和/或植物细胞中MybTF蛋白或其片段的表达来实现。 此外，本发明涉及具有增加的对真菌病原体的抗性的转基因植物，植物部分和/或植物细胞，特别是涉及芥菜的病原体，以及包含与序列相同或同源的序列的重组表达载体 编码MybTF蛋白。

公开(公告)号：US20160040182A1

公开(公告)日：2016-02-11

申请号：US14418659

申请日：2013-07-30

Applicant: BASF Plant Science Company GmbH

Inventor： Holger SCHULTHEISS ,  Ralf FLACHMANN

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8282 ,  C07K14/415

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an HCP5 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP5 protein.

Abstract translation: 本发明涉及一种在植物和/或植物细胞中增加对木瓜属（Pucciniales），优选家蚕科（Phacopsoraceae）的真菌病原体的抗性的方法。 与野生型植物，野生型植物部分和/或野生型植物细胞相比，通过增加植物，植物部分和/或植物细胞中HCP5蛋白或其片段的表达来实现。 此外，本发明涉及具有增加的抗真菌病原体的抗性的转基因植物，植物部分和/或植物细胞，特别是涉及Pucciniales，优选家庭Phacopsoraceae的病原体，以及包含相同序列的重组表达载体 或与编码HCP5蛋白的序列同源。

公开(公告)号：US20150361441A1

公开(公告)日：2015-12-17

申请号：US14840256

申请日：2015-08-31

Applicant: BASF Plant Science Company GmbH ,  Crop Functional Genomics Center

Inventor： Ana Isabel Sanz Molinero ,  Steven Vandenabeele ,  Christophe Reuzeau ,  Yang Do Choi ,  Ju Kon Kim

IPC: C12N15/82 ,  C12N9/16 ,  C07K14/415 ,  C12N9/10

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8273 ,  C12Y204/01015 ,  C12Y301/03012 ,  Y02A40/146

Abstract: The present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a CLC-like (Chloride Channel-like) polypeptide, an OsBURP-like (BURP-domain containing protein) polypeptide, an AP2/ERF polypeptide, or a protein fusion comprising a TPS (trehalose-6-phosphate synthase) and a TPP (trehalose-6-phosphate phosphatase) polypeptide, or modulating one or more nucleic acids encoding a TPS and a TPP enzyme, whether comprised in the same or in separate molecules. The present invention also concerns plants having modulated expression of a nucleic acid encoding a CLC-like polypeptide, an OsBURP-like polypeptide, an AP2/ERF polypeptide, or a TPP polypeptide, which plants have enhanced yield-related traits relative to control plants. Also provided are unknown TPS-encoding and TPP-encoding nucleic acids, and constructs comprising the same, and constructs a CLC-like, OsBURP-like, or AP2/ERF coding nucleic acid useful in performing the methods of the invention.

Abstract translation: 本发明涉及通过调节植物中编码CLC样（氯化物通道样）多肽，含OsBURP样（含BURP结构域的蛋白质）多肽）的核酸的表达来增强植物中产量相关性状的方法， AP2 / ERF多肽或包含TPS（海藻糖-6-磷酸合成酶）和TPP（海藻糖-6-磷酸磷酸酶）多肽的蛋白质融合物，或调节编码TPS和TPP酶的一种或多种核酸，无论是否 包含在相同或分开的分子中。 本发明还涉及已经调节了编码CLC样多肽，OsBURP样多肽，AP2 / ERF多肽或TPP多肽的核酸表达的植物，该植物相对于对照植物具有增强的产量相关性状。 还提供了未知的TPS编码和编码TPP的核酸，以及包含其的构建体，并构建可用于实施本发明方法的CLC样OsBURP样或AP2 / ERF编码核酸。

公开(公告)号：US20150317458A1

公开(公告)日：2015-11-05

申请号：US14650059

申请日：2013-12-05

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Katrin Lotz ,  Michael Leps ,  Rainer Lemke ,  Bjoern Junker ,  Falk Schreiber

IPC: G06F19/00 ,  C12N15/82

CPC classification number: G16C10/00 ,  A01H1/00 ,  C12N15/8216 ,  C12N15/8243 ,  C12N15/8254

Abstract: The present invention relates to a method for identifying at least one metabolic conversion step, the modulation of which increases the amount of a metabolite of interest in a plant cell, plant or plant part, said method comprising establishing a stoichiometric network model for the metabolism of the plant cell, plant or plant part including the synthesis pathway for the metabolite of interest, identifying at least one candidate metabolic conversion step by applying at least one algorithm of Growth-coupled Design, and validating the at least one candidate metabolic conversion step by a constraint-based modeling approach in the stoichiometric network model, wherein an increase in the metabolite of interest occurring in said constraint-based modeling approach is indicative for a metabolic conversion step, the modulation of which increases the amount of the metabolite of interest in the plant cell, plant or plant part. The present invention further relates to a method for generating a plant cell, plant or plant part which produces an increased amount of a metabolite of interest when compared to a control, said method comprising identifying a metabolic conversion step, the modulation of which increases a metabolite of interest in a plant cell, plant or plant part, by the method for identifying a metabolic conversion step and modulating the said metabolic conversion step such that the amount of the metabolite of interest is increased in vivo in a plant cell, plant or plant part.

Abstract translation: 本发明涉及用于鉴定至少一种代谢转化步骤的方法，所述代谢转化步骤的调节增加植物细胞，植物或植物部分中感兴趣的代谢物的量，所述方法包括建立用于代谢的化学计量网络模型 所述植物细胞，植物或植物部分包括所述感兴趣代谢物的合成途径，通过应用至少一种生长耦合设计算法来鉴定至少一种候选代谢转化步骤，以及通过以下步骤验证所述至少一种候选代谢转化步骤： 在化学计量网络模型中基于约束的建模方法，其中在所述基于约束的建模方法中发生的感兴趣代谢物的增加指示代谢转化步骤，其调节增加植物中感兴趣的代谢物的量 细胞，植物或植物部分。 本发明还涉及当与对照相比时产生植物细胞，植物或植物部分的方法，其产生增加的感兴趣代谢物的量，所述方法包括鉴定代谢转化步骤，其调节增加代谢物 感兴趣的植物细胞，植物或植物部分，通过鉴定代谢转化步骤的方法和调节所述代谢转化步骤，使得感兴趣的代谢物的量在植物细胞，植物或植物部分的体内增加 。