公开(公告)号：US10371692B2

公开(公告)日：2019-08-06

申请号：US15488432

申请日：2017-04-14

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Randy Davis

IPC: B81B1/00 ,  B82Y5/00 ,  G01N15/12 ,  C12Q1/6869 ,  G01N33/487

Abstract: A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

公开(公告)号：US10345290B2

公开(公告)日：2019-07-09

申请号：US16021664

申请日：2018-06-28

Applicant: Genia Technologies, Inc.

Inventor： Jennifer Hovis ,  Hui Tian ,  Roger J. A. Chen

IPC: G01N33/487

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

公开(公告)号：US10273536B2

公开(公告)日：2019-04-30

申请号：US15066117

申请日：2016-03-10

Applicant: Genia Technologies, Inc.

Inventor： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech ,  Hui Tian ,  Robert Yuan ,  John Foster ,  Roger Chen

IPC: G01N27/327 ,  C12Q1/6869 ,  C12M1/34 ,  G01N33/487 ,  G01N27/447

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

公开(公告)号：US20190119745A1

公开(公告)日：2019-04-25

申请号：US16238874

申请日：2019-01-03

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Timothy Kellogg CRAIG ,  Cynthia Ann CECH ,  Michael DORWART ,  Liv Elisabeth JENSEN ,  Marshall Winston PORTER ,  Christos TZITZILONIS ,  Alexander Hyun-min YANG

IPC: C12Q1/6869 ,  C07K14/31

CPC classification number: C12Q1/6869 ,  C07K14/31 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

公开(公告)号：US10246743B2

公开(公告)日：2019-04-02

申请号：US15385779

申请日：2016-12-20

Applicant: Genia Technologies, Inc.

Inventor： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech

IPC: G01N27/447 ,  C12Q1/6869 ,  C12M1/34 ,  G01N27/327 ,  G01N33/487

Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

公开(公告)号：US20190070796A1

公开(公告)日：2019-03-07

申请号：US16179800

申请日：2018-11-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: B29C67/20 ,  G01N33/487 ,  C12Q1/6869 ,  B82Y5/00 ,  B82Y40/00 ,  B82Y10/00

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US20190064107A1

公开(公告)日：2019-02-28

申请号：US16172488

申请日：2018-10-26

Applicant: Genia Technologies, Inc.

Inventor： Kevin Deierling ,  Roger J.A. Chen ,  David J. Fullagar

IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: A device for controlling, detecting, and measuring a molecular complex is disclosed. The device comprises a common electrode. The device further comprises a plurality of measurement cells. Each measurement cell includes a cell electrode and an integrator electronically coupled to the cell electrode. The integrator measures the current flowing between the common electrode and the cell electrode. The device further comprises a plurality of analog-to-digital converters, wherein an integrator from the plurality of measurement cells is electrically coupled to one analog-to-digital converter of the plurality of analog-to-digital converters.

公开(公告)号：US10102338B2

公开(公告)日：2018-10-16

申请号：US14864408

申请日：2015-09-24

Applicant: Genia Technologies, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  Bill Maney ,  Jayalakshmi Rajaraman

IPC: G01N33/48 ,  G01N33/50 ,  G06F19/22 ,  G01N33/487

Abstract: A system includes a plurality of nanopore cells. Data corresponding to nanopore states of the plurality of nanopore cells is received. The data is analyzed to determine a compressed output size of the data given at least one compression technique. It is determined whether the compressed output size exceeds a data budget. In the event it is determined that the compressed output size exceeds the data budget, the data is modified. The modified data is outputted.

公开(公告)号：US10060903B2

公开(公告)日：2018-08-28

申请号：US14534042

申请日：2014-11-05

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/447 ,  G01N27/22 ,  G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US10010852B2

公开(公告)日：2018-07-03

申请号：US15697904

申请日：2017-09-07

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen

IPC: G05D23/00 ,  B01J19/00 ,  G05D23/20

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.