-
公开(公告)号：US09670542B2
公开(公告)日：2017-06-06
申请号：US15400920
申请日：2017-01-06
Applicant: Keygene N.V.
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6827 , C12Q1/6846 , C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , C12Q1/6869 , C12Q2600/13 , G06F19/22 , C12Q2525/155 , C12Q2563/179 , C12Q2563/155 , C12Q2537/143
Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
-
公开(公告)号：US20170121706A1
公开(公告)日：2017-05-04
申请号：US15344815
申请日：2016-11-07
Applicant: Keygene N.V.
Inventor： Michiel Theodoor Jan DE BOTH , Tomoyuki FURUKAWA
IPC: C12N15/10
CPC classification number: C12N15/1024 , C12N15/102 , C12N15/1082
Abstract: The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises use of at least two oligonucleotides, each oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch of the first oligonucleotide is directed to a nucleotide at a position in the first strand of the duplex and the mismatch of the second oligonucleotide is directed to the nucleotide in the second strand that occupies the complementary position in the duplex acceptor DNA (e.g. forms a base-pair with the nucleotide in the first strand). These mismatches are located at specific positions within said oligonucleotides. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises oligonucleotides suitable for use in the method.
-
公开(公告)号：US09493820B2
公开(公告)日：2016-11-15
申请号：US14626822
申请日：2015-02-19
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
-
公开(公告)号：US09334536B2
公开(公告)日：2016-05-10
申请号：US14274591
申请日：2014-05-09
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Anker Preben Sørensen , Marco Gerardus Maria Van Schriek
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
-
公开(公告)号：US20150329906A1
公开(公告)日：2015-11-19
申请号：US14808761
申请日：2015-07-24
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Adrianus Johannes VAN TUNEN , Antoine Antonius Arnoldus Wilhelmus JANSSEN , Taco Peter JESSE
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2563/179 , C12Q2535/122 , C12Q2525/191 , C12Q2565/514 , C12Q2565/601 , C12Q2565/631
Abstract: The invention relates to a method for the determination of a genome sequence comprising the steps of providing a physical map of a sample genome by sequencing fragment ends of pooled BAC clones; providing a set of sequence reads from a sample genome generating a contig of the physical map and the sequence reads.
Abstract translation: 本发明涉及一种用于确定基因组序列的方法，包括以下步骤：通过测序合并的BAC克隆的片段末端来提供样品基因组的物理图谱; 从样本基因组提供一组序列读取，产生物理图和序列读数的重叠群。
-
Patent Agency Ranking
公开(公告)号：US20150315602A1
公开(公告)日：2015-11-05
申请号：US14648643
申请日：2013-11-29
Applicant: KEYGENE N.V.
Inventor： Paul Johan DIERGAARDE , Marinus Willem PRINS , Martin DE VOS
IPC: C12N15/82
CPC classification number: C12N15/8223
Abstract: Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising such trichome-specific promoter or a chimeric or vector comprising such trichome-specific promoter. The invention further provides methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.
Abstract translation: 本文提供了Trichome特异性植物启动子。 还提供了转基因细胞和生物体，特别是植物细胞和植物，其包含这样的毛细胞特异性启动子或包含这种毛发特异性启动子的嵌合或载体。 本发明还提供了使用毛发特异性启动子在细胞和生物体中表达核酸序列的方法。
-
公开(公告)号：US20150148241A1
公开(公告)日：2015-05-28
申请号：US14613849
申请日：2015-02-04
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Taco Peter JESSE
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
-
公开(公告)号：US20150059018A1
公开(公告)日：2015-02-26
申请号：US14352672
申请日：2012-10-19
Applicant: Keygene N.V.
CPC classification number: C12P7/02 , C12N9/0004 , C12N9/0006 , C12N9/16 , C12N15/8241 , C12N15/8286 , C12P17/04
Abstract: The present invention relates to nucleic acids sequences derived from Valeriana officinalis and/or Persicaria hydropiper and encoding drimenol synthase polypeptides. The present invention also provides the amino acid sequences of the polypeptides. The invention further provides host cells or organisms genetically modified to harbour the polynucleotides of the invention. A method to produce drimenol and/or a drimenol derivative by contacting farnesyl diphosphate with a polypeptide having a drimenol synthase activity is also part of this invention.
Abstract translation: 本发明涉及源自缬草和/或Persicaria hydropiper的核酸序列，并编码檀香醇合成酶多肽。 本发明还提供了多肽的氨基酸序列。 本发明还提供遗传修饰以携带本发明的多核苷酸的宿主细胞或生物体。 通过使法呢基二磷酸酯与具有萜醇合成酶活性的多肽接触来生产蓖麻醇和/或dr醇衍生物的方法也是本发明的一部分。
-
公开(公告)号：US20140315728A1
公开(公告)日：2014-10-23
申请号：US14318352
申请日：2014-06-27
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Anker Preben Sørensen , Marco Gerardus Maria Van Schriek
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
Abstract translation: 本发明涉及一种用于一种或多种样品中一种或多种遗传标记物的高通量发现，检测和基因分型的方法，其包括DNA的限制性内切核酸酶消化，衔接子连接，任选的预扩增，选择性扩增，合并 的扩增产物，对文库以足够的冗余进行测序，聚类，然后鉴定文库内和/或文库之间的遗传标记和确定遗传标记的（共）优势基因型。
-
140.发明申请公开(公告)号：US20140213462A1
公开(公告)日：2014-07-31
申请号：US14166218
申请日：2014-01-28
Applicant: Keygene N.V.
IPC: C12N15/10
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
Abstract translation: 本发明涉及通过在两个或多个样品上进行复杂性降低以产生两个或更多个文库，测序至少部分文库，对齐所鉴定的序列并确定任何推定的单核苷酸的方法，用于高通量鉴定单核苷酸多态性的方法 多态性，确认任何推定的单核苷酸多态性，产生用于确认的单核苷酸多态性的检测探针，将测试样品命名为相同的复杂性降低以提供测试文库并筛选测试文库以存在或不存在单核苷酸多态性 检测探头。
-
-
-
-
-
-
-
-
-
Search Results
181
records
(took 0.03 seconds)
