公开(公告)号：US4767622A

公开(公告)日：1988-08-30

申请号：US524919

申请日：1983-08-19

申请人： Miodrag Ristic ,  Mary L. Chilbert

发明人： Miodrag Ristic ,  Mary L. Chilbert

IPC分类号： C12P21/00 ,  A61K39/00 ,  A61K39/015 ,  C07K14/445 ,  C12N1/10 ,  C07G7/00

CPC分类号： C12N1/10 ,  C07K14/445 ,  A61K39/00 ,  Y10S530/822

摘要： Disclosed are vaccine compositions for use in developing protective immunity against infection by Plasmodium parasites. Soluble proteinaceous immunogens are isolated from the fluid culture medium of in vitro propagated plasmodial species parasites (e.g., P.falciparum) in mammalian erythrocyte culture supernatant or from washes, including hypotonic washes, of cultured erythrocytes parasitized by plasmodium. Immunogens so obtained have molecular weights in the range from about 35,000 daltons to about 85,000 daltons. Two principal immunogens of the invention have molecular weights of about 42,000 and 54,000 daltons, respectively. The water soluble immunogens are administered in a suitable carrier such as isotonic salt solution and in combination with a suitable adjuvant such as saponin or, preferably, aluminum hydroxide. Upon administration to vertebrate animals susceptible to plasmodial infection, vaccines according to the invention provoke immune responses protective against morbidity and mortality caused by, e.g., P.falciparum infection. Disclosed also are procedures for optimization of large scale plasmodial parasite growth in human erythrocyte cultures with accompanying development of late erythrocytic stage parasites in large numbers and optimization of isolatable quantities of culture medium supernatant and wash-derived immunogens.

摘要翻译： 公开了用于开发针对疟原虫感染的保护性免疫的疫苗组合物。 可溶性蛋白质免疫原从哺乳动物红细胞培养上清液中的体外繁殖的疟原虫寄生虫（例如恶性疟原虫）的流体培养基中分离出来，或从疟原虫寄生的培养的红细胞洗涤（包括低渗洗涤）中分离。 如此获得的免疫原具有在约35,000道尔顿至约85,000道尔顿范围内的分子量。 本发明的两种主要免疫原分别具有约42,000和54,000道尔顿的分子量。 水溶性免疫原在合适的载体例如等渗盐溶液中并与合适的佐剂如皂苷或优选氢氧化铝组合施用。 在给予对疟原虫感染敏感的脊椎动物的动物时，根据本发明的疫苗引发了针对例如恶性疟原虫感染引起的发病率和死亡率的免疫应答。 还公开了用于优化人类红细胞培养物中大规模的疟原虫寄生虫生长的程序，伴随着大量晚期红细胞阶段寄生虫的发展，并优化了可分离量的培养基上清液和洗涤来源的免疫原。

公开(公告)号：US4764370A

公开(公告)日：1988-08-16

申请号：US650282

申请日：1984-09-12

申请人： Patricia I. Fields ,  Constantine G. Haidaris ,  Frederick L. Heffron

发明人： Patricia I. Fields ,  Constantine G. Haidaris ,  Frederick L. Heffron

IPC分类号： C12N15/00 ,  A61K39/00 ,  A61K39/002 ,  A61K39/02 ,  A61K39/112 ,  C12N1/10 ,  C12N1/14 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N15/09 ,  C12N15/90 ,  C12R1/01 ,  C12R1/07 ,  C12R1/145 ,  C12R1/185 ,  C12R1/21 ,  C12R1/22 ,  C12R1/32 ,  C12R1/38 ,  C12R1/42 ,  C12R1/44 ,  C12R1/46 ,  C12R1/645 ,  C12R1/66 ,  C12R1/90 ,  C12N1/00 ,  A61K37/00

CPC分类号： A61K39/0002 ,  A61K39/002 ,  A61K39/02 ,  C12N15/90 ,  A61K2039/522 ,  Y10S435/879

摘要： A vaccine against a microbial pathogen comprised of a live, immunogenic but prototrophic and avirulent mutant strain of the selected microbial pathogen in an amount effective to confer immunity. A method of obtaining a vaccine that induces a heightened cellular and humoral immune response to one of a variety of microbial pathogens in a warm blooded animal. A method for isolation of an avirulent strain of a selected pathogenic microorganism.

摘要翻译： 一种针对微生物病原体的疫苗，其包含有效赋予免疫力的选定微生物病原体的活的，免疫原性的，营养不良的和无毒力的突变菌株。 一种获得在温血动物中诱导对各种微生物病原体之一的细胞和体液免疫应答增强的疫苗的方法。 一种分离所选病原微生物的无毒菌株的方法。

公开(公告)号：US20240141395A1

公开(公告)日：2024-05-02

申请号：US18498807

申请日：2023-10-31

申请人： FERMENTALG

发明人： Francois GODART ,  Adeline LAPENDRIE

IPC分类号： C12P7/6472 ,  A23L33/115 ,  C12N1/10

CPC分类号： C12P7/6472 ,  A23L33/115 ,  C12N1/105 ,  C12R2001/90

摘要： The present invention relates to an oil of microorganisms rich in docosahexaenoic acid (DHA, C22:6n3), comprising more than 60% of DHA relative to the total mass of fat and to the use thereof for human or animal feed, in particular for feeding infants, children, or pregnant or lactating women.

公开(公告)号：US20220177934A1

公开(公告)日：2022-06-09

申请号：US17533771

申请日：2021-11-23

申请人： MARA RENEWABLES CORPORATION

发明人： Violeta Ugalde ,  Zachary Sun ,  Anthony Windust ,  Roberto E. Armenta

IPC分类号： C12P7/6427 ,  A23K20/158 ,  C12N1/14 ,  C12P7/6472 ,  A23L33/115 ,  C12N1/10 ,  C12N1/12 ,  A23K20/147 ,  A23K50/00 ,  A23K10/18 ,  A23L33/135 ,  C12P7/6409 ,  C12P21/00 ,  C12P23/00

摘要： Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

公开(公告)号：US20220017929A1

公开(公告)日：2022-01-20

申请号：US17291608

申请日：2019-11-07

申请人： EVONIK OPERATIONS GMBH

发明人： Horst PRIEFERT ,  Jens SCHNEIDER ,  Joachim WINDAU

IPC分类号： C12P7/64 ,  C12N1/10

摘要： According to the invention, it has been found that when the amount of sulfate used in the culturing of PUFAs-producing cells is selected such that the sulfate concentration in the last phase of fermentation is always above zero, but at the same time always below the saturation limit of the cells, a biomass is obtained which has an increased proportion by mass of polyunsaturated fatty acids (PUFAs) in the final product, the process having at the same time a high product/substrate yield and space/time yield.

公开(公告)号：US20210309960A1

公开(公告)日：2021-10-07

申请号：US17266984

申请日：2019-08-09

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Tohru DAIRI ,  Yasuharu SATOH ,  Shohei HAYASHI ,  Mai NAKA ,  Tetsuro UJIHARA

IPC分类号： C12N1/10 ,  C12N15/70 ,  C12P7/64

摘要： An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.

公开(公告)号：US11110132B2

公开(公告)日：2021-09-07

申请号：US16675842

申请日：2019-11-06

申请人： Ohio State Innovation Foundation

发明人： Bradford McGwire

IPC分类号： A61K35/68 ,  A61K39/005 ,  C12N1/10 ,  A61K39/00

摘要： Disclosed herein is an avirulent live vaccine that involves a recombinant protozoan from the order Trypanosomatida having a knocked out or silenced cyclophilin gene, wherein the cyclophilin gene comprises T. cruzi cyclophilin 19 (TcCyp19) gene, or an orthologue thereof. Also disclosed is a method or inducing a protective immune response in a subject that involves administering to the subject a vaccine disclosed herein. Also disclosed is a method of treating or preventing Chagas disease, African trypanosomiasis, and/or leishmaniasis in a subject that involves administering to the subject a vaccine disclosed herein.

公开(公告)号：US20200216793A1

公开(公告)日：2020-07-09

申请号：US16824302

申请日：2020-03-19

申请人： Nancy Leland

发明人： Nancy Leland

IPC分类号： C12M1/00 ,  C12N13/00 ,  C12N1/20 ,  C12N1/12 ,  C12N1/10 ,  C12N1/02

摘要： Methods, devices and kits for the physical separation of plankton into its component parts utilizing phototactic behavior are described. The methods utilize positive phototactic behavior and negative contrast orientation of the zooplankton for maximal in situ separation of phytoplankton and zooplankton for use in further studies and evaluation of separation efficiency. The devices provide effective conditions for use in the separation of plankton into component parts.

公开(公告)号：US10689612B2

公开(公告)日：2020-06-23

申请号：US15300499

申请日：2015-04-02

申请人： Intervet inc.

发明人： Koen Gevers ,  Theodorus Petrus Maria Schetters

IPC分类号： C12N1/10 ,  C12N1/02 ,  B07B1/18 ,  B07B1/22 ,  B07B1/10

摘要： The invention pertains to a method to purify coccidial oocysts having dimensions between Dmin and Dmax from faeces comprising the steps of collecting the faeces containing the coccidial oocysts from host animals, diluting the faeces in an aqueous medium, separating a coarse fraction comprising macroscopic particulate matter from the diluted faeces and collecting an aqueous fraction containing the oocysts, characterised in that the method further comprises sieving the aqueous fraction over a first sieve deck having mesh openings to let the oocysts pass, to obtain an aqueous filtrate comprising the oocysts and a first residue comprising particles larger than the oocysts, and sieving the aqueous filtrate over a second sieve deck having mesh openings to obstruct passing of the oocysts through this sieve deck, to obtain a second residue comprising the purified oocysts and a waist filtrate comprising particles smaller than the oocysts. The invention also pertains to a system suitable for applying this method and to oocysts obtained therewith.

公开(公告)号：US10441646B2

公开(公告)日：2019-10-15

申请号：US15858574

申请日：2017-12-29

申请人： SANARIA INC.

发明人： Abraham G. Eappen ,  Stephen L. Hoffman

IPC分类号： A61K39/015 ,  A61K39/00 ,  A61K49/00 ,  C12N1/10

摘要： The application is directed to in vitro-reared Plasmodium sporozoites of human host range wherein sporogony from gametocyte stage to sporozoite stage is external to mosquitoes, and methods of producing the same. Provided herein are in vitro-reared infectious Plasmodium sporozoites (SPZ) of human host range, particularly P. falciparum, P. vivax, P. ovale, P. malariae, and P. knowlesi, wherein sporogony from gametocyte stage to sporozoite stage is external to mosquitoes, and methods of producing the same.