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    METHODS AND SYSTEMS FOR REDUCING PHASING ERRORS WHEN SEQUENCING NUCLEIC ACIDS USING TERMINATION CHEMISTRY
    151.
    发明申请
    METHODS AND SYSTEMS FOR REDUCING PHASING ERRORS WHEN SEQUENCING NUCLEIC ACIDS USING TERMINATION CHEMISTRY 有权

    公开(公告)号：US20210108254A1

    公开(公告)日：2021-04-15

    申请号：US17025763

    申请日：2020-09-18

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl HUBBELL

    IPC: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874

    Abstract: A method for nucleic acid sequencing may include disposing a plurality of template nucleic acid molecules in a plurality of defined spaces disposed on a sensor array, at least some of the plurality of template nucleic acid molecules having a sequencing primer and a polymerase operably bound therewith; advancing one or more nucleotide species over the plurality of template nucleic acid molecules with the sequencing primer and the polymerase operably bound therewith; measuring a signal generated by nucleotide incorporations resulting from advancing the one or more nucleotide species; and exposing the plurality of template nucleic acid molecules to a cleaving reagent subsequent to the advancing and measuring. The cleaving reagent can remove labeling reagents attached to the one or more nucleotide species. The advancing and measuring steps can be performed for different orders of the one or more nucleotide species prior to a subsequent exposing of the plurality of template nucleic acid molecules to the cleaving reagent.

    Methods and kits for extraction of DNA
    156.
    发明授权
    Methods and kits for extraction of DNA 有权

    公开(公告)号：US10961528B2

    公开(公告)日：2021-03-30

    申请号：US16011597

    申请日：2018-06-18

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Maxim G. Brevnov ,  Hemant S. Pawar ,  Manohar R. Furtado ,  Jaiprakash G. Shewale

    IPC: C12N15/10 ,  C12Q1/6806

    Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

    SYSTEMS AND METHODS FOR PROVIDING FLUIDIC ACCESS TO A FLOW CELL
    160.
    发明申请
    SYSTEMS AND METHODS FOR PROVIDING FLUIDIC ACCESS TO A FLOW CELL 有权

    公开(公告)号：US20210053058A1

    公开(公告)日：2021-02-25

    申请号：US16994289

    申请日：2020-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jon HOSHIZAKI ,  Patrick LEUNG ,  David MARRAN ,  Mark REED

    IPC: B01L3/00

    Abstract: An apparatus includes a fluidic coupler including an opening. A first port is in fluid communication with the opening and is to interface with an inlet of a flow cell of a sensor device. A second port is to interface with an outlet of the flow cell of the sensor device. A third port is in fluidic communication with the second port. The apparatus further includes a mechanical assembly moveable between a first position and a second position. The fluidic coupler is secured to the flow cell of the sensor device in the first position. The fluidic coupler is disengaged from the flow cell of the sensor device in the second position.

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