公开(公告)号：US10351907B2

公开(公告)日：2019-07-16

申请号：US14515741

申请日：2014-10-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC: C12Q1/6869 ,  B01J19/00 ,  B01F13/00 ,  B01F13/02 ,  B01L3/00 ,  G01N35/00 ,  G01N35/10 ,  C12Q1/6874 ,  G02B21/34

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

公开(公告)号：US08889596B2

公开(公告)日：2014-11-18

申请号：US13969195

申请日：2013-08-16

Applicant: Life Technologies Corporation

Inventor： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC: C12Q1/68 ,  G01N35/10 ,  B01J19/00 ,  B01L3/00 ,  B01F13/00 ,  B01F13/02 ,  G01N35/00 ,  G02B21/34

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

Abstract translation: 根据各种实施方案，提供了一种方法，其包括用洗涤溶液洗涤基底上的DNA包被的珠粒阵列以从基底去除堆叠的珠粒。 洗涤溶液可以在载体中包括惰性固体珠。 DNA包被的珠可以具有平均直径，并且洗涤溶液中的固体珠的平均直径可以是DNA包被的珠的直径的至少两倍。 洗涤可以形成脱落的DNA包被的珠粒和单层DNA包被的珠粒。 在一些实施方案中，用于形成阵列的第一珠粒与包含分子量约350Da或更低的PEG的聚（乙二醇）（PEG）溶液接触。 在一些实施例中，用于形成珠阵列的滑块被提供为用于对其进行成像的系统。

公开(公告)号：US11795503B2

公开(公告)日：2023-10-24

申请号：US16512276

申请日：2019-07-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC: C12Q1/6869 ,  G01N35/10 ,  B01L3/00 ,  G01N35/00 ,  G01N21/11 ,  B01F33/30 ,  B01F33/40 ,  B01J19/00 ,  C12Q1/6874 ,  G02B21/34 ,  G01N21/05

CPC classification number: C12Q1/6869 ,  B01F33/30 ,  B01F33/403 ,  B01J19/0046 ,  B01L3/50273 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N21/11 ,  G01N35/00069 ,  G01N35/0099 ,  G01N35/1002 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00466 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00869 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0851 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  G01N21/05 ,  G01N35/1011 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

公开(公告)号：US10809225B2

公开(公告)日：2020-10-20

申请号：US14789795

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald L. Cicero ,  Marc Glazer ,  Yufang Wang ,  Christina E. Inman ,  Jeremy Gray ,  Joseph Koscinski ,  James A. Ball ,  Phil Waggoner ,  Alexander Mastroianni

IPC: G01N27/414

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

公开(公告)号：US20210102916A1

公开(公告)日：2021-04-08

申请号：US17060680

申请日：2020-10-01

Applicant: Life Technologies Corporation

Inventor： Ronald L. Cicero ,  Marc Glazer ,  Yufang Wang ,  Christina E. Inman ,  Jeremy Gray ,  Joseph Koscinski ,  James A. Ball ,  Phil Waggoner ,  Alexander Mastroianni

IPC: G01N27/414

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

公开(公告)号：US10066260B2

公开(公告)日：2018-09-04

申请号：US14774662

申请日：2014-03-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald L. Cicero ,  Christina E. Inman ,  Paul M. Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q2523/101 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2547/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2523/301 ,  C12Q2523/32

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US09852919B2

公开(公告)日：2017-12-26

申请号：US14676697

申请日：2015-04-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christina E. Inman ,  Alexander Mastroianni ,  Wolfgang Hinz ,  Shifeng Li ,  Scott C. Benson

IPC: H01L21/00 ,  H01L21/311 ,  G01N27/414

CPC classification number: H01L21/31105 ,  G01N27/4145 ,  H01L21/31144

Abstract: A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemFETs), depositing a dielectric layer over the chemFETs in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemFETs, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemFETs. The protective layer is at least one of a polymer, photoresist material, noble metal, copper oxide, and zinc oxide. The protective protective layer is removed using at least one of sodium hydroxide, organic solvent, aqua regia, ammonium carbonate, hydrochloric acid, acetic acid, and phosphoric acid.