公开(公告)号：US10961571B2

公开(公告)日：2021-03-30

申请号：US16223053

申请日：2018-12-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei Xi ,  Keith P. Bjornson ,  Stephen P. Hendricks

IPC: C12Q1/686 ,  C12Q1/6853

Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.

公开(公告)号：US10093973B2

公开(公告)日：2018-10-09

申请号：US15169854

申请日：2016-06-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen P. Hendricks ,  Michael Phelan ,  Marian Peris ,  Cheng-Yao Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Amy Castillo

IPC: C12P19/34 ,  C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

公开(公告)号：US20230055565A1

公开(公告)日：2023-02-23

申请号：US17935406

申请日：2022-09-26

Applicant: Life Technologies Corporation

Inventor： Stephen P. Hendricks ,  Michael Phelan ,  Marian Peris ,  Cheng-Yao Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Amy Castillo

IPC: C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

公开(公告)号：US09365839B2

公开(公告)日：2016-06-14

申请号：US14710160

申请日：2015-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen P. Hendricks ,  Michael Phelan ,  Marian Peris ,  Cheng-Yao Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Amy Castillo

IPC: C12N9/12 ,  C07H19/20 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

Abstract translation: 本文公开了显示改变的聚合酶活性的修饰聚合酶组合物，其可用于多种生物应用。 本文还公开了制备和使用这些组合物的方法。 在一些实施方案中，组合物显示改变的性质，其可以增强其在各种生物应用中的效用。 这种改变的性质可以包括例如改变的核苷酸结合亲和力，改变的核苷酸掺入动力学，改变的光稳定性和/或改变的纳米颗粒耐受性，以及本文公开的一系列其它性质。

公开(公告)号：US20190194721A1

公开(公告)日：2019-06-27

申请号：US16223053

申请日：2018-12-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei Xi ,  Keith P. Bjornson ,  Stephen P. Hendricks

IPC: C12Q1/686 ,  C12Q1/6853

CPC classification number: C12Q1/686 ,  C12Q1/6853 ,  C12Q2565/101 ,  C12Q2563/107 ,  C12Q2525/301 ,  C12Q2565/107 ,  C12Q2561/113 ,  C12Q2545/113 ,  C12Q2565/1015

Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.

公开(公告)号：US12163188B2

公开(公告)日：2024-12-10

申请号：US17935406

申请日：2022-09-26

Applicant: Life Technologies Corporation

Inventor： Stephen P. Hendricks ,  Michael Phelan ,  Marian Peris ,  Cheng-Yao Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Amy Castillo

IPC: C12N9/12 ,  C07H19/20 ,  C12N9/96 ,  C12Q1/6818 ,  C12Q1/6869 ,  G01N21/64 ,  G01N33/58

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

公开(公告)号：US11453909B2

公开(公告)日：2022-09-27

申请号：US16123104

申请日：2018-09-06

Applicant: Life Technologies Corporation

Inventor： Stephen P. Hendricks ,  Michael Phelan ,  Marian Peris ,  Cheng-Yao Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Amy Castillo

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6869 ,  C07H19/20 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.