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    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain
    161.
    发明申请
    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain 审中-公开
    消除或减少丝状真菌菌株中基因表达的方法

    公开(公告)号：US20140287475A1

    公开(公告)日：2014-09-25

    申请号：US14255642

    申请日：2014-04-17

    Applicant: Novozymes A/S ,  Novozymes, Inc.

    Inventor： Howard Brody ,  Suchindra Maiyuran ,  Hiroaki Udagawa

    IPC: C12P1/02

    CPC classification number: C12P1/02 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

    Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.

    Abstract translation: 本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）将丝状真菌菌株的双链可转录核酸构建体插入基因组中，所述双链可转录核酸构建体包含第一核苷酸序列，其包含 可操作地连接到靶基因的同源编码区的启动子和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二核苷酸 序列相对于第一个核苷酸序列处于相反方向; 和（b）通过在有利于产生干扰RNA的条件下培养丝状真菌菌株诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。 本发明还涉及丝状真菌菌株以及在这种丝状真菌菌株中生产目的生物物质的方法。

    Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
    163.
    发明申请
    Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same 有权
    具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸

    公开(公告)号：US20140223608A1

    公开(公告)日：2014-08-07

    申请号：US14347475

    申请日：2012-09-28

    Applicant: Novozymes, Inc.

    Inventor： Yu Zhang ,  Junxin Duan ,  Lan Tang

    IPC: C12N9/42 ,  C12P19/02 ,  C12P19/14

    CPC classification number: C12N9/2437 ,  C12P19/14 ,  C12P21/02

    Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

    Abstract translation: 本发明提供了具有纤维素分解增强活性的分离的多肽和编码该多肽的多核苷酸。 本发明还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum
    168.
    发明申请
    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum 审中-公开
    在镰孢镰刀菌缺乏酶突变体中产生多肽的方法

    公开(公告)号：US20140147847A1

    公开(公告)日：2014-05-29

    申请号：US14168766

    申请日：2014-01-30

    Applicant: Novozymes, Inc.

    Inventor： Jeffrey Shasky ,  Wendy Yoder

    IPC: C12N15/80 ,  C12P21/00

    CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

    Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

    Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

    Methods of increasing secretion of polypeptides having biological activity
    169.
    发明授权
    Methods of increasing secretion of polypeptides having biological activity 有权

    公开(公告)号：US08735549B2

    公开(公告)日：2014-05-27

    申请号：US14024292

    申请日：2013-09-11

    Applicant: Novozymes, Inc.

    Inventor： Sandra Merino

    IPC: C12P21/00 ,  C12P21/04 ,  C07K1/00 ,  C12P21/02

    CPC classification number: C12N9/2437 ,  C07K1/00 ,  C07K2319/00 ,  C07K2319/036 ,  C12N9/2434 ,  C12N9/2445 ,  C12N15/80 ,  C12P19/02 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01004

    Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

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