Abstract:
The present invention relates to methods for cryopreserving plant cells and to methods for recovering viable plant cells from long or short term cryopreservation. Plant cells to be cryopreserved can be grown in culture and pretreated with a solution containing an cryoprotective agent and, optionally, a stabilizer. Stabilizers are preferably membrane stabilizers such as ethylene inhibitors, oxygen radical scavengers and divalent cations. Cells can also be stabilized by subjecting the culture to a heat shock. Pretreated cells are acclimated to a reduced temperature and loaded with a cryoprotective agent such as DMSO, propylene glycol or polyethylene glycol. Loaded cells are incubated with a vitrification solution which, for example, comprises a solution with a high concentration of the cryoprotective agent. Vitrified cells retain less than about 20% water content and can be frozen at cryopreservation temperatures for long periods of time without significantly altering the genotypic or phenotypic character of the cells. Plant cells may also be cryopreserved by lyophilizing cells prior to exposure to a vitrification solution. The combination of lyophilization and vitrification removes about 80% to about 95% of the plant cell's water. Cells can be successfully cryopreserved for long periods of time and viably recovered. The invention also relates to methods for the recovery of viable plant cells from cryopreservation. Cells are thawed to about room temperature and incubated in medium containing a cryoprotective agent and a stabilizer. The cryoprotective agent is removed and the cells successfully incubated and recovered in liquid or semi-solid growth medium. The invention also relates to the cryopreserved cells and to viable plant cells which have been recovered from long or short term cryopreservation.
Abstract:
An asymmetric double gate metal-oxide semiconductor field-effect transistor (MOSFET) includes a first fin formed on a substrate; a second fin formed on the substrate; a first gate formed adjacent first sides of the first and second fins, the first gate being doped with a first type of impurity; and a second gate formed between second sides of the first and second fins, the second gate being doped with a second type of impurity. An asymmetric all-around gate MOSFET includes multiple fins; a first gate structure doped with a first type of impurity and formed adjacent a first side of one of the fins; a second gate structure doped with the first type of impurity and formed adjacent a first side of another one of the fins; a third gate structure doped with a second type of impurity and formed between two of the fins; and a fourth gate structure formed at least partially beneath one or more of the fins.
Abstract:
A method of forming a gate for a Fin Field Effect Transistor (FinFET) is provided. The method includes forming a first layer of material over a fin and forming a second layer over the first layer. The second layer includes either Ti or TiN. The method further includes forming a third layer over the second layer. The third layer includes an anti-reflective coating. The method also includes etching the first, second and third layers to form the gate for the FinFET.
Abstract:
Methods are provided for cryopreserving plant cells and to methods for recovering viable plant cells from long or short term cryopreservation. Plant cells to be cryopreserved can be grown in culture and pretreated with a solution containing an cryorotective agent and a stabilizer. Pretreated cells are acclimated to a reduced temperature and loaded with a cryoprotective agent such as DMSO, propylene glycol or polyethylene glycol. Loaded cells are incubated with a vitrification solution which, for example, comprises a solution with a high concentration of the cryoprotective agent. Vitrified cells retain less than about 20% water content and can be frozen at cryopreservation temperatures for long periods of time without significantly altering the genotypic or phenotypic character of the cells. Plant cells may also be cryopreserved by lyophilizing cells to a preferable water content of about 40% to about 60% by weight prior to exposure to a vitrification solution or loading agent. The combination of lyophilization and vitrification or loading removes about 75% to about 95% of the plant cell's water. Cells can be successfully cryopreserved for long periods of time and viably recovered. Also provided are methods for the recovery of viable plant cells from cryopreservation. Cells are thawed to about room temperature and incubated in medium containing, a cryoprotective agent and a stabilizer. The cryoprotective agent is removed and the cells successfully incubated and recovered in liquid or semi-solid growth medium.
Abstract:
A silicon-on-insulator(SOI) transistor. The SOI transistor having a source and a drain having a body disposed therebetween, the source being implanted with germanium to form an area of silicon-germanium adjacent a source/body junction in a lower portion of the source, the area of silicon-germanium in the source forming a hereto junction along a lower portion of the source/body junction.
Abstract:
A method for fabricating a semiconductor device, involving: forming a gate stack on a substrate; depositing a material layer on the gate stack; etching the material layer, thereby forming a dielectric capsulate layer on the gate stack; forming a pair of shallow source/drain extensions in a first region of the substrate by implanting a plurality of first dopant ions at a tilt angle with a horizontal offset defined by a thickness of the dielectric capsulate layer; and forming at least one spacer on the dielectric capsulate layer; forming deep source/drain contact junctions in a second region of the substrate by vertically implanting a plurality of second dopant ions below the first region with no tilt and with a horizontal offset defined by a thickness of the at least one spacer.
Abstract:
Short-channel effects are controlled by forming abrupt, graded halo profiles. Embodiments include sequentially forming deep source/drain regions, ion implanting to form first deep amorphized regions, ion implanting an impurity into the first deep amorphized regions to form first deep halo implants, laser thermal annealing to recrystallize the first deep amorphized regions and activate the deep halo regions, ion implanting to form second shallow amorphized regions within the deep halo regions, ion implanting an impurity into the second shallow amorphous regions to form second shallow halo implants and laser thermal annealing to recrystallize the second shallow amorphous regions and to activate the shallow halo regions. Embodiments further include forming shallow source/drain extensions within the shallow halo implants and laser thermal annealing to activate the shallow source/drain extensions.
Abstract:
A method of fabricating a semiconductor device, having a locally-formed metal oxide high-k gate insulator, involving: nitriding a substrate to form a thin silicon nitride layer; depositing a thin metal film on the thin silicon nitride layer; forming a localized metal oxide layer from the thin metal film, wherein the a thick nitride layer is deposited on the thin metal film, the thick nitride layer is patterned, the at least one exposed thin metal film portion is locally oxidized, by heating, wherein the oxidizing is performed by local laser irradiation; forming a gate stack having the localized metal oxide layer and a gate electrode, wherein the a thick gate material is deposited in the electrode cavity and on the localized metal oxide layer; the thick gate material is polished, thereby forming the gate electrode; and the thick nitride layer along with the at least one covered thin metal film portion are removed, thereby forming the gate stack; and completing fabrication of the device, and a device thereby formed.
Abstract:
A method of manufacturing an integrated circuit utilizes solid phase epitaxy to form an elevated source region and an elevated drain region. The method includes providing an amorphous semiconductor material and crystallizing the amorphous semiconductor material without damaging a high-k gate dielectric layer. The gate structure includes L-shaped liners. The semiconductor material can be silicided. A shallow source drain implant can also be provided.
Abstract:
A MOSFET has a drain region, a source region, and a channel region, and the MOSFET initially has a gate comprised of a capping layer on a polysilicon structure disposed on a gate dielectric over the channel region. A drain silicide and a source silicide having a first silicide thickness are formed in the drain region and the source region, respectively. A dielectric layer is deposited over the drain region, the source region, and the gate. The dielectric layer is polished until the capping layer of the gate is exposed such that the capping layer and the first dielectric layer are substantially level. The capping layer on the polysilicon structure of the gate is etched away such that the top of the polysilicon structure is exposed. A top portion of the first dielectric layer is etched away until sidewalls at a top portion of the polysilicon structure are exposed. A polysilicon spacer is formed at the exposed sidewalls at the top portion of the polysilicon structure. A silicidation metal is deposited on the top of the polysilicon structure that is exposed and on the polysilicon spacer. A silicidation anneal is performed with the silicidation metal and the polysilicon structure that is exposed and the polysilicon spacer to form a gate silicide having a second silicide thickness on top of the polysilicon structure of the gate. Because the gate silicide is formed with the added polysilicon spacer at the exposed sidewalls of the polysilicon structure, the gate silicide has a width that is larger than a width of the polysilicon structure of the gate. In addition, the gate silicide is formed in a separate step from the step for forming the drain silicide and the source silicide such that the gate silicide may have a larger thickness and be comprised of different metal silicide material from that of the drain silicide and the source silicide.