Cryopreservation of plant cells
    181.
    发明申请
    Cryopreservation of plant cells 审中-公开
    植物细胞的冷冻保存

    公开(公告)号:US20050158699A1

    公开(公告)日:2005-07-21

    申请号:US10871705

    申请日:2004-06-21

    CPC classification number: A01N1/0284 A01N1/0221

    Abstract: The present invention relates to methods for cryopreserving plant cells and to methods for recovering viable plant cells from long or short term cryopreservation. Plant cells to be cryopreserved can be grown in culture and pretreated with a solution containing an cryoprotective agent and, optionally, a stabilizer. Stabilizers are preferably membrane stabilizers such as ethylene inhibitors, oxygen radical scavengers and divalent cations. Cells can also be stabilized by subjecting the culture to a heat shock. Pretreated cells are acclimated to a reduced temperature and loaded with a cryoprotective agent such as DMSO, propylene glycol or polyethylene glycol. Loaded cells are incubated with a vitrification solution which, for example, comprises a solution with a high concentration of the cryoprotective agent. Vitrified cells retain less than about 20% water content and can be frozen at cryopreservation temperatures for long periods of time without significantly altering the genotypic or phenotypic character of the cells. Plant cells may also be cryopreserved by lyophilizing cells prior to exposure to a vitrification solution. The combination of lyophilization and vitrification removes about 80% to about 95% of the plant cell's water. Cells can be successfully cryopreserved for long periods of time and viably recovered. The invention also relates to methods for the recovery of viable plant cells from cryopreservation. Cells are thawed to about room temperature and incubated in medium containing a cryoprotective agent and a stabilizer. The cryoprotective agent is removed and the cells successfully incubated and recovered in liquid or semi-solid growth medium. The invention also relates to the cryopreserved cells and to viable plant cells which have been recovered from long or short term cryopreservation.

    Abstract translation: 本发明涉及用于冷冻保存植物细胞的方法和从长期或短期冷冻保存中回收活的植物细胞的方法。 待冷冻保存的植物细胞可以在培养物中生长并用含有冷冻保护剂和任选的稳定剂的溶液预处理。 稳定剂优选为膜稳定剂,例如乙烯抑制剂,氧自由基清除剂和二价阳离子。 也可以通过使培养物受热休克来稳定细胞。 预处理的细胞适应于降低的温度,并加载冷冻保护剂如DMSO,丙二醇或聚乙二醇。 将加载的细胞与玻璃化溶液一起孵育,所述玻璃化溶液例如包含具有高浓度冷冻保护剂的溶液。 玻璃化细胞保留少于约20%的水含量,并且可以在冷冻保存温度下长时间冷冻,而不显着改变细胞的基因型或表型特征。 植物细胞也可以在暴露于玻璃化溶液之前由冻干细胞冷冻保存。 冻干和玻璃化的组合消除了植物细胞水的约80%至约95%。 细胞可以成功地冷冻保存长时间,有效地恢复。 本发明还涉及从冷冻保存中回收活的植物细胞的方法。 将细胞解冻至约室温,并在含有冷冻保护剂和稳定剂的培养基中孵育。 除去冷冻保护剂,并将细胞在液体或半固体生长培养基中成功培养和回收。 本发明还涉及冷冻保存的细胞和已经从长期或短期冷冻保存中回收的活的植物细胞。

    Asymmetrical double gate or all-around gate MOSFET devices and methods for making same
    182.
    发明授权
    Asymmetrical double gate or all-around gate MOSFET devices and methods for making same 失效
    非对称双栅极或全栅极MOSFET器件及其制造方法

    公开(公告)号:US06800885B1

    公开(公告)日:2004-10-05

    申请号:US10385652

    申请日:2003-03-12

    CPC classification number: H01L29/785 H01L29/42392 H01L29/66795 H01L29/7856

    Abstract: An asymmetric double gate metal-oxide semiconductor field-effect transistor (MOSFET) includes a first fin formed on a substrate; a second fin formed on the substrate; a first gate formed adjacent first sides of the first and second fins, the first gate being doped with a first type of impurity; and a second gate formed between second sides of the first and second fins, the second gate being doped with a second type of impurity. An asymmetric all-around gate MOSFET includes multiple fins; a first gate structure doped with a first type of impurity and formed adjacent a first side of one of the fins; a second gate structure doped with the first type of impurity and formed adjacent a first side of another one of the fins; a third gate structure doped with a second type of impurity and formed between two of the fins; and a fourth gate structure formed at least partially beneath one or more of the fins.

    Abstract translation: 非对称双栅极金属氧化物半导体场效应晶体管(MOSFET)包括在基板上形成的第一鳍片; 在所述基板上形成的第二翅片; 形成在所述第一和第二鳍片的第一侧附近的第一栅极,所述第一栅极掺杂有第一类型的杂质; 以及形成在所述第一和第二鳍片的第二侧之间的第二栅极,所述第二栅极掺杂有第二类型的杂质。 非对称全栅极MOSFET包括多个鳍片; 掺杂有第一类型杂质的第一栅极结构,并且邻近其中一个鳍片的第一侧形成; 掺杂有第一类型杂质的第二栅极结构,并且与另一个鳍片的第一侧相邻地形成; 掺杂有第二类杂质并形成在两个鳍之间的第三栅极结构; 以及至少部分地在一个或多个翅片下方形成的第四门结构。

    Etch stop layer for etching FinFET gate over a large topography
    183.
    发明授权
    Etch stop layer for etching FinFET gate over a large topography 有权
    蚀刻停止层,用于在大地形上蚀刻FinFET栅极

    公开(公告)号:US06787476B1

    公开(公告)日:2004-09-07

    申请号:US10632989

    申请日:2003-08-04

    Abstract: A method of forming a gate for a Fin Field Effect Transistor (FinFET) is provided. The method includes forming a first layer of material over a fin and forming a second layer over the first layer. The second layer includes either Ti or TiN. The method further includes forming a third layer over the second layer. The third layer includes an anti-reflective coating. The method also includes etching the first, second and third layers to form the gate for the FinFET.

    Abstract translation: 提供了一种形成Fin场效应晶体管(FinFET)的栅极的方法。 该方法包括在翅片上形成第一层材料,并在第一层上形成第二层。 第二层包括Ti或TiN。 该方法还包括在第二层上形成第三层。 第三层包括抗反射涂层。 该方法还包括蚀刻第一,第二和第三层以形成用于FinFET的栅极。

    Cryopreservation of plant cells
    184.
    发明授权
    Cryopreservation of plant cells 失效
    植物细胞的冷冻保存

    公开(公告)号:US06753182B1

    公开(公告)日:2004-06-22

    申请号:US08780449

    申请日:1997-01-08

    CPC classification number: A01N3/00

    Abstract: Methods are provided for cryopreserving plant cells and to methods for recovering viable plant cells from long or short term cryopreservation. Plant cells to be cryopreserved can be grown in culture and pretreated with a solution containing an cryorotective agent and a stabilizer. Pretreated cells are acclimated to a reduced temperature and loaded with a cryoprotective agent such as DMSO, propylene glycol or polyethylene glycol. Loaded cells are incubated with a vitrification solution which, for example, comprises a solution with a high concentration of the cryoprotective agent. Vitrified cells retain less than about 20% water content and can be frozen at cryopreservation temperatures for long periods of time without significantly altering the genotypic or phenotypic character of the cells. Plant cells may also be cryopreserved by lyophilizing cells to a preferable water content of about 40% to about 60% by weight prior to exposure to a vitrification solution or loading agent. The combination of lyophilization and vitrification or loading removes about 75% to about 95% of the plant cell's water. Cells can be successfully cryopreserved for long periods of time and viably recovered. Also provided are methods for the recovery of viable plant cells from cryopreservation. Cells are thawed to about room temperature and incubated in medium containing, a cryoprotective agent and a stabilizer. The cryoprotective agent is removed and the cells successfully incubated and recovered in liquid or semi-solid growth medium.

    Abstract translation: 提供用于冷冻保存植物细胞的方法和用于从长期或短期冷冻保存中回收活的植物细胞的方法。 待冷冻保存的植物细胞可以在培养物中生长并用含有冷冻保护剂和稳定剂的溶液预处理。 预处理的细胞适应于降低的温度,并加载冷冻保护剂如DMSO,丙二醇或聚乙二醇。 将加载的细胞与玻璃化溶液一起孵育,所述玻璃化溶液例如包含具有高浓度冷冻保护剂的溶液。 玻璃化细胞保留少于约20%的水含量,并且可以在冷冻保存温度下长时间冷冻,而不显着改变细胞的基因型或表型特征。 在暴露于玻璃化溶液或加载剂之前,植物细胞还可以通过冻干细胞冷冻保存至约40%至约60%重量的优选水含量。 冷冻干燥和玻璃化或加载的组合去除植物细胞水的约75%至约95%。 细胞可以成功地冷冻保存长时间,有效地恢复。 还提供了从冷冻保存中回收活的植物细胞的方法。 将细胞解冻至约室温,并在含有冷冻保护剂和稳定剂的培养基中孵育。 除去冷冻保护剂,并将细胞在液体或半固体生长培养基中成功培养和回收。

    Silicon-on-insulator (SOI) transistor having partial hetero source/drain junctions fabricated with high energy germanium implantation.
    185.
    发明授权
    Silicon-on-insulator (SOI) transistor having partial hetero source/drain junctions fabricated with high energy germanium implantation. 有权
    具有用高能量锗注入制造的部分异质源极/漏极结的绝缘体上硅(SOI)晶体管。

    公开(公告)号:US06706614B1

    公开(公告)日:2004-03-16

    申请号:US10145953

    申请日:2002-05-15

    CPC classification number: H01L29/66742 H01L29/78618 H01L29/78684

    Abstract: A silicon-on-insulator(SOI) transistor. The SOI transistor having a source and a drain having a body disposed therebetween, the source being implanted with germanium to form an area of silicon-germanium adjacent a source/body junction in a lower portion of the source, the area of silicon-germanium in the source forming a hereto junction along a lower portion of the source/body junction.

    Abstract translation: 绝缘体上硅(SOI)晶体管。 具有源极和漏极的SOI晶体管具有设置在其间的主体,源被注入锗以形成邻近源极的下部的源极/主体结的硅 - 锗的区域,硅 - 锗的面积 源沿着源/体结的下部形成本结。

    Method of fabricating a semiconductor device having a MOS transistor with a high dielectric constant material
    186.
    发明授权
    Method of fabricating a semiconductor device having a MOS transistor with a high dielectric constant material 有权
    制造具有高介电常数材料的MOS晶体管的半导体器件的方法

    公开(公告)号:US06686248B1

    公开(公告)日:2004-02-03

    申请号:US09825658

    申请日:2001-04-03

    Applicant: Bin Yu

    Inventor: Bin Yu

    Abstract: A method for fabricating a semiconductor device, involving: forming a gate stack on a substrate; depositing a material layer on the gate stack; etching the material layer, thereby forming a dielectric capsulate layer on the gate stack; forming a pair of shallow source/drain extensions in a first region of the substrate by implanting a plurality of first dopant ions at a tilt angle with a horizontal offset defined by a thickness of the dielectric capsulate layer; and forming at least one spacer on the dielectric capsulate layer; forming deep source/drain contact junctions in a second region of the substrate by vertically implanting a plurality of second dopant ions below the first region with no tilt and with a horizontal offset defined by a thickness of the at least one spacer.

    Abstract translation: 一种制造半导体器件的方法,包括:在衬底上形成栅叠层; 在栅极堆叠上沉积材料层; 蚀刻材料层,从而在栅叠层上形成电介质封装层; 通过以与介电封装层的厚度限定的水平偏移的倾斜角度注入多个第一掺杂剂离子,在衬底的第一区域中形成一对浅源极/漏极延伸部分; 以及在介电封装层上形成至少一个间隔物; 通过在第一区域的下方垂直地注入多个第二掺杂剂离子并且没有由至少一个间隔物的厚度限定的水平偏移,在衬底的第二区域中形成深源/漏接触结。

    Dual laser anneal for graded halo profile
    187.
    发明授权
    Dual laser anneal for graded halo profile 有权
    双激光退火用于渐变晕轮廓

    公开(公告)号:US06642122B1

    公开(公告)日:2003-11-04

    申请号:US10254850

    申请日:2002-09-26

    Applicant: Bin Yu

    Inventor: Bin Yu

    Abstract: Short-channel effects are controlled by forming abrupt, graded halo profiles. Embodiments include sequentially forming deep source/drain regions, ion implanting to form first deep amorphized regions, ion implanting an impurity into the first deep amorphized regions to form first deep halo implants, laser thermal annealing to recrystallize the first deep amorphized regions and activate the deep halo regions, ion implanting to form second shallow amorphized regions within the deep halo regions, ion implanting an impurity into the second shallow amorphous regions to form second shallow halo implants and laser thermal annealing to recrystallize the second shallow amorphous regions and to activate the shallow halo regions. Embodiments further include forming shallow source/drain extensions within the shallow halo implants and laser thermal annealing to activate the shallow source/drain extensions.

    Abstract translation: 短通道效应通过形成突变的渐变晕轮廓来控制。 实施例包括顺序形成深源极/漏极区域,离子注入以形成第一深非晶化区域,将杂质离子注入到第一深非晶化区域中以形成第一深光晕植入物,激光热退火以使第一深非晶化区域再结晶并激活深 卤素区域,离子注入以在深晕区域内形成第二浅非晶化区域,离子将杂质注入第二浅非晶区域以形成第二浅光晕植入物和激光热退火以使第二浅无定形区域再结晶并激活浅光晕 地区。 实施例还包括在浅光晕植入物内形成浅源极/漏极延伸部分以及激光热退火以激活浅源极/漏极延伸部分。

    Method of fabricating a semiconductor device having a metal oxide high-k gate insulator by localized laser irradiation and a device thereby formed
    188.
    发明授权
    Method of fabricating a semiconductor device having a metal oxide high-k gate insulator by localized laser irradiation and a device thereby formed 有权
    通过局部激光照射制造具有金属氧化物高k栅极绝缘体的半导体器件的方法和由此形成的器件

    公开(公告)号:US06531368B1

    公开(公告)日:2003-03-11

    申请号:US09825750

    申请日:2001-04-03

    Applicant: Bin Yu

    Inventor: Bin Yu

    Abstract: A method of fabricating a semiconductor device, having a locally-formed metal oxide high-k gate insulator, involving: nitriding a substrate to form a thin silicon nitride layer; depositing a thin metal film on the thin silicon nitride layer; forming a localized metal oxide layer from the thin metal film, wherein the a thick nitride layer is deposited on the thin metal film, the thick nitride layer is patterned, the at least one exposed thin metal film portion is locally oxidized, by heating, wherein the oxidizing is performed by local laser irradiation; forming a gate stack having the localized metal oxide layer and a gate electrode, wherein the a thick gate material is deposited in the electrode cavity and on the localized metal oxide layer; the thick gate material is polished, thereby forming the gate electrode; and the thick nitride layer along with the at least one covered thin metal film portion are removed, thereby forming the gate stack; and completing fabrication of the device, and a device thereby formed.

    Abstract translation: 一种制造具有局部形成的金属氧化物高k栅极绝缘体的半导体器件的方法,包括:氮化氮化硅层,形成薄的氮化硅层; 在薄氮化硅层上沉积薄金属薄膜; 从所述薄金属膜形成局部金属氧化物层,其中所述厚氮化物层沉积在所述薄金属膜上,所述厚氮化物层被图案化,所述至少一个暴露的金属薄膜部分通过加热而局部氧化,其中 通过局部激光照射进行氧化; 形成具有局部金属氧化物层和栅电极的栅极堆叠,其中厚栅极材料沉积在电极腔和局部金属氧化物层上; 对厚栅极材料进行抛光,从而形成栅电极; 并且去除厚氮化物层与至少一个覆盖的薄金属膜部分,从而形成栅极堆叠; 并完成该装置的制造以及由此形成的装置。

    Low temperature process for a transistor with elevated source and drain
    189.
    发明授权
    Low temperature process for a transistor with elevated source and drain 失效
    具有升高的源极和漏极的晶体管的低温工艺

    公开(公告)号:US06524920B1

    公开(公告)日:2003-02-25

    申请号:US09779988

    申请日:2001-02-09

    Applicant: Bin Yu

    Inventor: Bin Yu

    Abstract: A method of manufacturing an integrated circuit utilizes solid phase epitaxy to form an elevated source region and an elevated drain region. The method includes providing an amorphous semiconductor material and crystallizing the amorphous semiconductor material without damaging a high-k gate dielectric layer. The gate structure includes L-shaped liners. The semiconductor material can be silicided. A shallow source drain implant can also be provided.

    Abstract translation: 集成电路的制造方法利用固相外延形成升高的源极区域和升高的漏极区域。 该方法包括提供非晶半导体材料并使非晶半导体材料结晶而不损坏高k栅介质层。 门结构包括L形衬垫。 半导体材料可以被硅化。 还可以提供浅源极漏极植入物。

    Fabrication of a wide metal silicide on a narrow polysilicon gate structure

    公开(公告)号:US06507078B1

    公开(公告)日:2003-01-14

    申请号:US10131858

    申请日:2002-04-25

    Applicant: Bin Yu

    Inventor: Bin Yu

    Abstract: A MOSFET has a drain region, a source region, and a channel region, and the MOSFET initially has a gate comprised of a capping layer on a polysilicon structure disposed on a gate dielectric over the channel region. A drain silicide and a source silicide having a first silicide thickness are formed in the drain region and the source region, respectively. A dielectric layer is deposited over the drain region, the source region, and the gate. The dielectric layer is polished until the capping layer of the gate is exposed such that the capping layer and the first dielectric layer are substantially level. The capping layer on the polysilicon structure of the gate is etched away such that the top of the polysilicon structure is exposed. A top portion of the first dielectric layer is etched away until sidewalls at a top portion of the polysilicon structure are exposed. A polysilicon spacer is formed at the exposed sidewalls at the top portion of the polysilicon structure. A silicidation metal is deposited on the top of the polysilicon structure that is exposed and on the polysilicon spacer. A silicidation anneal is performed with the silicidation metal and the polysilicon structure that is exposed and the polysilicon spacer to form a gate silicide having a second silicide thickness on top of the polysilicon structure of the gate. Because the gate silicide is formed with the added polysilicon spacer at the exposed sidewalls of the polysilicon structure, the gate silicide has a width that is larger than a width of the polysilicon structure of the gate. In addition, the gate silicide is formed in a separate step from the step for forming the drain silicide and the source silicide such that the gate silicide may have a larger thickness and be comprised of different metal silicide material from that of the drain silicide and the source silicide.

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