公开(公告)号：US20120209028A1

公开(公告)日：2012-08-16

申请号：US13392744

申请日：2010-08-18

申请人： Jeffrey S. Tolan ,  Brian Foody ,  Vijay Anand

发明人： Jeffrey S. Tolan ,  Brian Foody ,  Vijay Anand

IPC分类号： C07C51/42

CPC分类号： C12F3/00 ,  C07C51/43 ,  C07C51/445 ,  Y02E50/16 ,  C07C53/08

摘要： A process for recovering a volatile carboxylic acid from an aqueous stream comprising same, the process comprising the steps of: (i) steam stripping the carboxylic acid from the aqueous stream, which aqueous stream is produced by a conversion process using a lignocellulosic feedstock as a substrate, the steam stripping comprising contacting the aqueous stream with steam by flowing the aqueous stream and the steam countercurrent to one another, thereby producing a vapour stream comprising vapourized carboxylic acid and steam and a stripped aqueous stream; (ii) extracting the vapourized carboxylic acid with an organic solvent by contacting the vapour stream with the organic solvent to produce (a) a stream comprising the organic solvent and the carboxylic acid and (b) the steam at least substantially depleted of the carboxylic acid, wherein the organic solvent has an atmospheric boiling point of at least about 150° C. and is insoluble in water; (iii) returning the steam from step (ii) to the steam stripping step (step i) to further strip the carboxylic acid from the aqueous stream; and (iv) separating the carboxylic acid from the organic solvent.

摘要翻译： 一种从包含其的含水流中回收挥发性羧酸的方法，该方法包括以下步骤：（i）从水流中汽提汽提羧酸，该水流通过使用木质纤维素原料的转化方法生产， 蒸汽汽提包括通过使水流和蒸汽逆流地相互流动使水流与蒸汽接触，从而产生包含蒸发的羧酸和蒸汽和汽提的水流的蒸气流; （ii）通过使蒸气流与有机溶剂接触来提取挥发的羧酸，以产生（a）包含有机溶剂和羧酸的物流，和（b）至少基本上不含羧酸的蒸汽 其中所述有机溶剂的常压沸点为至少约150℃，并且不溶于水; （iii）将来自步骤（ii）的蒸汽返回到汽提步骤（步骤i），以进一步从水流中分离出羧酸; 和（iv）从有机溶剂中分离出羧酸。

公开(公告)号：US20120052534A1

公开(公告)日：2012-03-01

申请号：US13216368

申请日：2011-08-24

申请人： Peter J.E. Harlick ,  Wei Zheng

发明人： Peter J.E. Harlick ,  Wei Zheng

IPC分类号： C12P19/02 ,  C12M1/00

CPC分类号： C12M45/06 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  Y02E50/16

摘要： Provided is a process for the enzymatic hydrolysis of cellulose to produce glucose from a pretreated cellulosic feedstock. The process comprises providing an aqueous slurry of the pretreated cellulosic feedstock that has a water content that is less than about 140% of the maximum water holding capacity of the pretreated cellulosic feedstock. The aqueous slurry of the pretreated cellulosic feedstock is fed to one or more unmixed hydrolysis reactors and hydrolyzed with cellulase enzymes therein. In the unmixed hydrolysis reactor(s), the cellulase enzymes hydrolyze a portion of the cellulose to produce soluble sugars, thereby producing a mixture of partially hydrolyzed cellulose containing soluble sugars. The hydrolysis of the cellulose to glucose is continued by feeding the mixture of partially hydrolyzed cellulose to one or more mixed hydrolysis reactors. Also provided are systems for carrying out the foregoing enzymatic hydrolysis.

摘要翻译： 提供了一种纤维素酶水解从预处理的纤维素原料产生葡萄糖的方法。 该方法包括提供预处理的纤维素原料的含水浆料，其含水量小于预处理的纤维素原料的最大保水能力的约140％。 将预处理的纤维素原料的含水浆料进料到一个或多个未混合的水解反应器中，并用其中的纤维素酶水解。 在未混合的水解反应器中，纤维素酶水解一部分纤维素以产生可溶性糖，从而产生含有可溶性糖的部分水解的纤维素的混合物。 通过将部分水解的纤维素的混合物喂入一个或多个混合水解反应器来继续将纤维素水解成葡萄糖。 还提供了用于进行上述酶水解的系统。

公开(公告)号：US20110207192A1

公开(公告)日：2011-08-25

申请号：US13032127

申请日：2011-02-22

申请人： Gary M. Pigeau ,  Jan-Maarten A. Geertman

发明人： Gary M. Pigeau ,  Jan-Maarten A. Geertman

IPC分类号： C12P7/02 ,  C12P7/06

CPC分类号： C12P7/06 ,  C12P7/10 ,  C12P7/14 ,  C12P7/18 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention relates to a method for producing a fermentation product from a sugar hydrolysate. The method comprises fermenting the sugar hydrolysate in a fermentation system with yeast to produce a fermentation broth comprising a fermentation product; introducing acid and an oxidant, such as chlorine dioxide, to the fermentation system so as to expose microbial contaminants in the fermentation system at one or more stages to chlorine dioxide and a pH of less than 3.0; and recovering the fermentation product. In one example of the invention, a yeast slurry obtained from a yeast recycle step is treated with acid and the oxidant.

摘要翻译： 本发明涉及从糖水解产物生产发酵产物的方法。 该方法包括在发酵系统中用酵母发酵糖水解产物以产生包含发酵产物的发酵液; 将酸和氧化剂（例如二氧化氯）引入发酵系统，以便在一个或多个阶段将发酵系统中的微生物污染物暴露于二氧化氯和小于3.0的pH; 并回收发酵产物。 在本发明的一个实例中，从酵母再循环步骤获得的酵母浆液用酸和氧化剂处理。

公开(公告)号：US20110159560A1

公开(公告)日：2011-06-30

申请号：US12981890

申请日：2010-12-30

申请人： Jennifer Donaldson ,  Jan-Maarten A. Geertman ,  Glenn D. Munkvold ,  Gary Pigeau ,  Philippe J. Dufresne ,  Loreta Gudynaite-Savitch

发明人： Jennifer Donaldson ,  Jan-Maarten A. Geertman ,  Glenn D. Munkvold ,  Gary Pigeau ,  Philippe J. Dufresne ,  Loreta Gudynaite-Savitch

IPC分类号： C12P7/10 ,  C12P7/04 ,  C12N1/00

CPC分类号： C12N15/81 ,  C12N15/01 ,  C12N15/52 ,  C12P7/08 ,  C12P7/10 ,  Y02E50/16

摘要： The present invention relates to novel xylose-fermenting yeast strains (for example, yeast of the genus Saccharomyces, e.g., S. cerevisiae) with an enhanced ability to ferment the xylose (and/or another pentose sugar) present in a lignocellulosic hydrolysate to a fermentation product(s) (for example, an alcohol (e.g., ethanol) or a sugar alcohol (e.g., xylitol)).

摘要翻译： 本发明涉及具有增强木质纤维素水解产物中存在的木糖（和/或其他戊糖）的能力的新型木糖发酵酵母菌株（例如酿酒酵母属酵母，例如酿酒酵母） 发酵产物（例如，醇（例如乙醇）或糖醇（例如木糖醇））。

公开(公告)号：US20100221819A1

公开(公告)日：2010-09-02

申请号：US12762589

申请日：2010-04-19

申请人： Brian Foody ,  Ziyad Rahme ,  Jeffrey S. Tolan

发明人： Brian Foody ,  Ziyad Rahme ,  Jeffrey S. Tolan

IPC分类号： C12M1/00

CPC分类号： C12P19/14 ,  C12M45/09 ,  C12P19/02 ,  C12P19/04 ,  Y02E50/16 ,  Y02E50/17

摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product from a pre-treated cellulosic feedstock is provided. The process comprises introducing an aqueous slurry of the pre-treated cellulosic feedstock at the bottom of a hydrolysis reactor. Axial dispersion in the reactor is limited by avoiding mixing and maintaining an average slurry flow velocity of about 0.1 to about 20 feet per hour, such that the undissolved solids flow upward at a rate slower than that of the liquid. Cellulase enzymes are added to the aqueous slurry before or during the step of introducing. An aqueous stream comprising hydrolysis product and unhydrolyzed solids is removed from the hydrolysis reactor. Also provided are enzyme compositions which comprise cellulase enzymes and flocculents for use in the process. In addition, a kit comprising cellulase enzymes and flocculent is provided.

摘要翻译： 提供了一种纤维素酶水解从预处理的纤维素原料产生水解产物的方法。 该方法包括在水解反应器的底部引入预处理的纤维素原料的含水浆料。 通过避免混合并保持约0.1至约20英尺/小时的平均浆料流速，使得未溶解的固体以比液体慢的速率向上流动，来限制反应器中的轴向分散。 在引入步骤之前或期间，将纤维素酶加入到含水浆料中。 从水解反应器中除去包含水解产物和未水解固体的水流。 还提供了包含用于该方法的纤维素酶和絮凝剂的酶组合物。 另外，提供了包含纤维素酶和絮状物的试剂盒。

公开(公告)号：US20100184151A1

公开(公告)日：2010-07-22

申请号：US12686485

申请日：2010-01-13

申请人： Jeffrey S. Tolan ,  Brian Foody

发明人： Jeffrey S. Tolan ,  Brian Foody

IPC分类号： C12P19/00 ,  C12P7/04

CPC分类号： C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

摘要： A method for producing glucose from a lignocellulosic feedstock is provided. The method comprises pretreating the lignocellulosic feedstock with acid to produce a pretreated feedstock composition. A calcium-containing stream is provided that comprises calcium that is obtained from the lignocellulosic feedstock and a calcium carbonate-containing stream is obtained by precipitation of the calcium from the calcium-containing stream. The pH of the pretreated feedstock is adjusted with (a) the calcium carbonate-containing stream; (b) a calcium hydroxide-containing stream that is derived from said calcium carbonate-containing stream by subjecting said calcium carbonate-containing stream to calcination; or (c) a combination of the calcium carbonate-containing stream and the calcium hydroxide-containing stream. The pH adjustment results in a neutralized pretreated lignocellulosic feedstock having a pH between about 3 and about 9 and enzymatic hydrolysis of the neutralized, pretreated lignocellulosic feedstock is then conducted with cellulase enzymes to produce the glucose.

摘要翻译： 提供了一种从木质纤维原料生产葡萄糖的方法。 该方法包括用酸处理木质纤维素原料以产生预处理的原料组合物。 提供含钙流，其包含从木质纤维素原料获得的钙，并且通过从含钙流中沉淀钙获得含碳酸钙的流。 用（a）含碳酸钙的料流调节预处理原料的pH; （b）通过使所述含碳酸钙的料流进行煅烧从含有所述含碳酸钙的料流中得到的含氢氧化钙的料流; 或（c）含碳酸钙的料流和含氢氧化钙的料流的组合。 pH调节导致pH为约3至约9的中和预处理木质纤维素原料，然后用纤维素酶进行中和的预处理的木质纤维素原料的酶水解以产生葡萄糖。

公开(公告)号：US20090209009A1

公开(公告)日：2009-08-20

申请号：US12305110

申请日：2007-06-22

申请人： Jeffrey Tolan ,  Theresa White ,  John Tomashek

发明人： Jeffrey Tolan ,  Theresa White ,  John Tomashek

IPC分类号： C12P19/02 ,  C12N9/42 ,  C12N9/38

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02P20/582

摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. During the hydrolysis, both the cellulase enzyme and β-glucosidase enzyme bind to the fiber solids. The hydrolysis is performed in a solids-retaining hydrolysis reactor so that unhydrolyzed fiber solids and bound enzyme are retained in the reactor longer than the aqueous phase of the slurry.

摘要翻译： 提供了纤维素酶水解以产生包含来自预处理的木质纤维素原料的葡萄糖和用于该方法的酶的水解产物的方法。 该方法包括用纤维素酶，一种或多于一种β-葡糖苷酶和用于将β-葡糖苷酶与含水浆料中存在的纤维固体结合的结合剂来水解预处理的木质纤维素原料的含水浆料。 在水解期间，纤维素酶和β-葡糖苷酶都结合纤维固体。 水解在固体保持水解反应器中进行，使得未水解的纤维固体和结合的酶比浆液的水相保留在反应器中。

公开(公告)号：US20090061484A1

公开(公告)日：2009-03-05

申请号：US12201374

申请日：2008-08-29

申请人： Brian R. Scott ,  Christopher Hill ,  John Tomashek ,  Chengsong Liu

发明人： Brian R. Scott ,  Christopher Hill ,  John Tomashek ,  Chengsong Liu

IPC分类号： C12P21/04 ,  C12P19/14 ,  C12N9/42

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.

摘要翻译： 提供了用于将预处理的木质纤维素原料水解成可溶性糖的酶混合物。 酶混合物包含约0.25至约0.83（w / w）的分数浓度（fEG4）的EG4，分数浓度（fSwo1）为约0至约0.66（w / w）的溶血素，和分数浓度的Cip1 （fCip1）相对于酶混合物中存在的所有辅助酶测量为0至约0.33。 还提供了使用酶混合物将预处理的木质纤维素原料转化为可溶性糖的方法，以及使用和生产这种酶混合物的方法。

公开(公告)号：US20090056707A1

公开(公告)日：2009-03-05

申请号：US12199976

申请日：2008-08-28

申请人： Brian E. Foody ,  Jeffrey S. Tolan

发明人： Brian E. Foody ,  Jeffrey S. Tolan

IPC分类号： C13J1/06

CPC分类号： C05D3/02 ,  B01J39/04 ,  B01J49/06 ,  C01B17/96 ,  C01D5/00 ,  C01F5/24 ,  C01F11/18 ,  C05C3/00 ,  C05D1/02 ,  C05F5/004 ,  C05F5/006 ,  C13B10/04 ,  C13B20/06 ,  C13B20/14 ,  C13B20/144 ,  C13K1/02 ,  C13K1/04 ,  Y02A40/209 ,  Y02A40/211 ,  C05D5/00

摘要： The present invention provides a process for obtaining a product stream comprising one or more sulfate salts of potassium, sodium or ammonium from a sugar stream resulting from [processing a lignocellulosic feedstock, said sugar stream which includes calcium sulfate and one or more of these sulfate salts. The process comprises (i) treating the sugar stream to remove calcium, thereby producing a sugar stream containing substantially no calcium, and obtaining a salt stream comprising a calcium salt;(ii) choosing a feed stream that is either (a) a clarified salt stream derived from the salt stream of step (i) after removal of calcium therefrom; or (b) the sugar stream containing substantially no calcium that is produced in step (i); (iii) introducing the feed stream chosen in step (ii) to an ion exchange bed; and (iv) regenerating the ion exchange resin bed of step (iii) with sulfuric acid to produce the product stream.

摘要翻译： 本发明提供了一种从由加工木质纤维素原料得到的糖料流中获得含有一种或多种钾，钠或铵的硫酸盐的产物流的方法，所述糖流包括硫酸钙和一种或多种这些硫酸盐。 该方法包括（i）处理糖流以除去钙，从而产生基本上不含钙的糖料流，并获得包含钙盐的盐流;（ii）选择（a）澄清盐 在从其中除去钙之后，从步骤（i）的盐流中得到的物流; 或（b）在步骤（i）中基本上不含钙的糖料流; （iii）将步骤（ii）中选择的进料流引入离子交换床; 和（iv）用硫酸再生步骤（iii）的离子交换树脂床以产生产物流。

公开(公告)号：US10889795B2

公开(公告)日：2021-01-12

申请号：US15779001

申请日：2016-11-24

申请人： Iogen Energy Corporation

发明人： Stephen A. Rowland ,  Chris Mallalieu ,  Ryan Taylor ,  Ziyad Rahme

IPC分类号： C08H8/00 ,  C12M1/00 ,  C10L1/02 ,  C13K1/02 ,  C12P7/10 ,  C12P7/16 ,  C12M1/33 ,  C12M1/34

摘要： Discharging pretreated biomass from a pretreatment reactor and mixing the discharged pretreated biomass with a cooling liquid in a vessel provides a cooled slurry having a consistency that is less than about 12 wt %. Since the consistency is relatively low, the cooled slurry may be pumped to a higher elevation using standard pumping equipment. At the higher elevation, the cooled slurry may be separated into a first stream comprising a liquid component of the slurry and a second other stream comprising a solid component of the slurry (e.g., having a consistency between about 15 wt % and 40 about wt %). The solid component may be fed to an inlet of a hydrolysis reactor, while the liquid component may be fed to a cooling system that provides a cooled stream. The cooled stream may then be cycled back to the vessel.