公开(公告)号：US20060091011A1

公开(公告)日：2006-05-04

申请号：US11204773

申请日：2005-08-15

Applicant: Changsheng Liu ,  Thomas Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas Kane ,  Qingbo Li

IPC: C07K1/26 ,  G01N27/447

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

公开(公告)号：US06863791B1

公开(公告)日：2005-03-08

申请号：US09676526

申请日：2000-10-02

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: G01N21/64 ,  G01N27/447 ,  G01N33/483 ,  G01N33/50 ,  G06K9/00 ,  G01N27/26

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，本发明的系统允许使用不同的染料组来标记在分离的泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US06375819B1

公开(公告)日：2002-04-23

申请号：US09373909

申请日：1999-08-13

Applicant: Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

Inventor： Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

IPC: G01N2726

CPC classification number: G01N27/44721 ,  G01N27/44782

Abstract: An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Abstract translation: 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

公开(公告)号：US20110232356A1

公开(公告)日：2011-09-29

申请号：US13053189

申请日：2011-03-21

Applicant: Changsheng LIU ,  Hequan Zhao

Inventor： Changsheng LIU ,  Hequan Zhao

IPC: G01N33/00 ,  G01N27/447

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，该系统允许使用不同的染料组来标记在单独泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US20110220508A1

公开(公告)日：2011-09-15

申请号：US13048846

申请日：2011-03-15

Applicant: Changsheng LIU ,  Thomas E. KANE ,  Qingbo LI

Inventor： Changsheng LIU ,  Thomas E. KANE ,  Qingbo LI

IPC: G01N27/447

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends is spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量滴定塔的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US20100147692A1

公开(公告)日：2010-06-17

申请号：US12634661

申请日：2009-12-09

Applicant: Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

IPC: G01N27/00

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends is spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量滴定塔的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US06926815B2

公开(公告)日：2005-08-09

申请号：US09843918

申请日：2001-04-30

Applicant: Changsheng Liu ,  Qingbo Li

Inventor： Changsheng Liu ,  Qingbo Li

IPC: C08F220/56 ,  G01N27/447 ,  G01N27/441 ,  G01N27/453

CPC classification number: G01N27/44747

Abstract: This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.

Abstract translation: 本发明涉及一种具有至少一种含有主要共聚单体和至少一种次级共聚单体的无规线性共聚物的凝胶基质的电泳分离介质，其中共聚单体沿共聚物链无规分布。 主要共聚单体是提供凝胶基质的主要物理，化学和筛分性质的丙烯酰胺或丙烯酰胺衍生物。 至少一种次级共聚单体赋予共聚物链固有的物理，化学或筛分特性。 初级和次级共聚单体以足以引发优化电泳性能的所需性质的比例存在。 本发明还涉及使用该凝胶基质分离生物分子的混合物的方法，制备新型电泳分离介质的方法和填充有电泳分离介质的毛细管。

公开(公告)号：US06630063B1

公开(公告)日：2003-10-07

申请号：US09413355

申请日：1999-10-06

Applicant: Qingbo Li ,  Songsan Zhou ,  Changsheng Liu

Inventor： Qingbo Li ,  Songsan Zhou ,  Changsheng Liu

IPC: G01N2726

CPC classification number: G01N27/44721 ,  G01N27/44782

Abstract: A capillary electrophoresis system comprises capillaries positioned in parallel to each other forming a plane. The capillaries are configured to allow samples to migrate. A light source is configured to illuminate the capillaries and the samples therein. This causes the samples to emit light. A lens is configured to receive the light emitted by the samples and positioned directly over a first group of the capillaries and obliquely over a second group of the capillaries. The light source is further configured to illuminate the second group of capillaries more than the first group of the capillaries such that amount of light received by the lens from the first group of capillaries is substantially identical to amount of light received from the second group of capillaries when an identical amount of the samples is migrating through the first and second group capillaries.

Abstract translation: 毛细管电泳系统包括彼此平行地形成平面的毛细管。 毛细管被配置为允许样品迁移。 光源被配置为照亮毛细管和其中的样品。 这样会使样品发光。 透镜被配置为接收由样品发射的光并且直接定位在第一组毛细管上并且倾斜地位于第二组毛细管上。 光源还被配置为比第一组毛细管照亮第二组毛细管，使得透镜从第一组毛细管接收的光量与从第二组毛细血管接收的光量基本相同 当相同量的样品通过第一和第二组毛细管迁移时。

公开(公告)号：US6063251A

公开(公告)日：2000-05-16

申请号：US866308

申请日：1997-05-30

Applicant: Thomas E. Kane ,  John R. Kernan ,  Changsheng Liu ,  Qingbo Li

Inventor： Thomas E. Kane ,  John R. Kernan ,  Changsheng Liu ,  Qingbo Li

IPC: G01N27/447

CPC classification number: G01N27/44782

Abstract: An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. Electrical crosstalk between neighboring capillaries is attenuated using a protective tubing formed from an electrically insulative material. This crosstalk attenuation is provided in the form of sheathing, either around individual capillaries, or around bundles of spaced apart capillaries. Crosstalk attenuation may be enhanced by passing a nonconductive gas or liquid through lumens formed in the protective tubing, in which lumens the capillary tubes are present.

Abstract translation: 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 使用由电绝缘材料形成的保护管，相邻毛细管之间的电气串扰被衰减。 这种串扰衰减以围绕单个毛细管或围绕分开的毛细管束的护套形式提供。 可以通过使非导电气体或液体通过在保护管中形成的内腔（其中存在毛细管的管腔）来增强串扰衰减。

公开(公告)号：US6027627A

公开(公告)日：2000-02-22

申请号：US105988

申请日：1998-06-29

Applicant: Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

Inventor： Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

IPC: G01N27/447 ,  G01N27/26 ,  G01N27/477

CPC classification number: G01N27/44782

Abstract: An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Abstract translation: 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。