公开(公告)号：US20050186214A1

公开(公告)日：2005-08-25

申请号：US11021438

申请日：2004-12-21

Applicant: Bin Liu ,  James Marks

Inventor： Bin Liu ,  James Marks

IPC: A61K39/395 ,  C07K16/30 ,  C12P21/08 ,  G01N33/574

CPC classification number: C07K16/3069 ,  A61K2039/505 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/77

Abstract: This invention provides novel prostate cancer specific internalizing human antibodies. The antibodies are useful by themselves to prevent growth and/or proliferation of prostate cancer cells. The antibodies can also be formulated as chimeric molecules to direct an effector (e.g. a cytotoxin, an imaging reagent, a drug, etc.) to a prostate tumor site.

Abstract translation: 本发明提供了新型前列腺癌特异性内化人抗体。 抗体本身可用于预防前列腺癌细胞的生长和/或增殖。 抗体还可以配制成嵌合分子以将效应物（例如细胞毒素，成像试剂，药物等）引导至前列腺肿瘤部位。

公开(公告)号：US20050037339A1

公开(公告)日：2005-02-17

申请号：US10855755

申请日：2004-05-26

Applicant: James Marks ,  Marie Poul ,  Baltazar Becerril

Inventor： James Marks ,  Marie Poul ,  Baltazar Becerril

IPC: G01N33/68 ,  A61K38/00 ,  A61K39/395 ,  A61K47/48 ,  A61P37/02 ,  C07K14/01 ,  C07K16/28 ,  C07K16/32 ,  C12N15/09 ,  C12N15/10 ,  C12N15/86 ,  C12Q1/70 ,  C40B20/08 ,  C40B30/04 ,  C40B40/02 ,  C40B50/06 ,  G01N33/15 ,  G01N33/50 ,  G01N33/554 ,  G01N33/566 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C12N15/1037 ,  A61K38/00 ,  A61K47/6843 ,  C07K14/005 ,  C07K16/2863 ,  C07K16/2881 ,  C07K16/32 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/77 ,  C07K2319/02 ,  C12N15/86 ,  C12N2795/10022

Abstract: This invention provides methods of selecting antibodies that are internalized into target cells. The methods generally involve contacting target cells with one or more members of an antibody phage display library. The members of the phage display library are also contacted with cells of a subtractive cell line. The target cells are then washed to remove the subtractive cell line cells and members of the phage display library that are non-specifically bound or weakly bound to the target cells. The target cells are cultured under conditions where members of the phage display library can be internalized if bound to an internalizing marker and internalized members of the phage display library are then identified.

Abstract translation: 本发明提供了选择内化于靶细胞中的抗体的方法。 所述方法通常涉及使靶细胞与抗体噬菌体展示文库的一个或多个成员接触。 噬菌体展示文库的成员也与减数细胞系的细胞接触。 然后洗涤靶细胞以除去非特异性结合或弱结合到靶细胞的消减细胞系细胞和噬菌体展示文库的成员。 靶细胞在噬菌体展示文库的成员可以内化的条件下培养，如果结合到内化标记物，然后鉴定噬菌体展示文库的内化成员。

公开(公告)号：US20070148774A1

公开(公告)日：2007-06-28

申请号：US11555519

申请日：2006-11-01

Applicant: John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

Inventor： John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

IPC: C12N15/86 ,  C12N15/74

CPC classification number: C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US20060263801A1

公开(公告)日：2006-11-23

申请号：US11361312

申请日：2006-02-24

Applicant: James Marks ,  Ulrik Nielsen ,  Dimitri Kirpotin

Inventor： James Marks ,  Ulrik Nielsen ,  Dimitri Kirpotin

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: G01N33/5011 ,  C40B30/04 ,  G01N33/5008 ,  G01N33/5432 ,  G01N33/566 ,  G01N33/6845 ,  G01N33/6854 ,  G01N33/6863

Abstract: This invention provides methods of identifying ligands that are internalized into a cell. The methods typically involve i) contacting the cell with a reporter non-covalently coupled to a ligand; ii) dissociating the reporter from the ligand and removing dissociated reporter from the surface of the cell; and iii) detecting the reporter within said cell (if any is present) where the presence of the reporter within said cell indicates that the ligand binds to an internalizing receptor and is internalized.

公开(公告)号：US20060099205A1

公开(公告)日：2006-05-11

申请号：US11154103

申请日：2005-06-15

Applicant: Gregory Adams ,  Eva Horak ,  Louis Weiner ,  James Marks

Inventor： Gregory Adams ,  Eva Horak ,  Louis Weiner ,  James Marks

IPC: A61K39/395 ,  C07K16/44 ,  C07K16/28

CPC classification number: A61K39/395 ,  A61K51/109 ,  A61K2039/505 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/622 ,  G01N2333/71

Abstract: Bispecific single chain antibody molecules are disclosed which may be used to advantage to treat various forms of cancer associated with the overexpression of members of the EGFR protein family.

Abstract translation: 公开了双特异性单链抗体分子，其可用于治疗与EGFR蛋白家族的成员的过表达相关的各种形式的癌症。

公开(公告)号：US06868736B2

公开(公告)日：2005-03-22

申请号：US10371871

申请日：2003-02-21

Applicant: Takeo Sawatari ,  Alex Klooster ,  Dean M. Aslam ,  Yuping Lin ,  James Marks

Inventor： Takeo Sawatari ,  Alex Klooster ,  Dean M. Aslam ,  Yuping Lin ,  James Marks

IPC: G01L7/08 ,  G01L9/00 ,  G02B6/32 ,  G02B6/38 ,  G01L1/24 ,  F16C11/00

CPC classification number: G02B6/3869 ,  G01L9/0042 ,  G01L9/0047 ,  G01L9/0077 ,  G02B6/32 ,  G02B6/3855

Abstract: The fiber optic pressure sensing system includes a sensor housing formed using MEMS processing. The sensor housing has ribs and grooves in both horizontal and vertical directions relative to the surface to allow the membrane to flex in a consistent manner. The flexing of the membrane allows the pedestal to be repeatedly positioned in response to pressure acting on the extension of the sensor head and membrane.

Abstract translation: 光纤压力传感系统包括使用MEMS处理形成的传感器外壳。 传感器外壳在水平和垂直方向上具有相对于表面的肋和槽，以允许膜以一致的方式弯曲。 膜的弯曲允许基座响应于作用在传感器头和膜的延伸部上的压力而重复定位。

公开(公告)号：US20140271478A1

公开(公告)日：2014-09-18

申请号：US14239124

申请日：2012-08-17

Applicant: Stephen Nishimura ,  Jianlong Lou ,  Jody Lynn Baron ,  James Marks

Inventor： Stephen Nishimura ,  Jianlong Lou ,  Jody Lynn Baron ,  James Marks

IPC: C07K16/28 ,  A61K49/00

CPC classification number: A61K49/00 ,  A01K2207/15 ,  A01K2217/075 ,  A61K2039/505 ,  C07K14/70546 ,  C07K16/2839 ,  C07K2317/24 ,  C07K2317/30 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92

Abstract: Provided herein are antibodies with high affinity for the β8 subunit of αβ8.

Abstract translation: 本文提供了对α＆bgr的8亚基具有高亲和力的抗体; 8。

公开(公告)号：US20100027553A1

公开(公告)日：2010-02-04

申请号：US12498076

申请日：2009-07-06

Applicant: Timothy Hardisty ,  Anthony James Marks ,  Christopher Michael Nott

Inventor： Timothy Hardisty ,  Anthony James Marks ,  Christopher Michael Nott

IPC: H04L12/56

CPC classification number: H04L63/10 ,  H04L12/462 ,  H04L65/1023 ,  H04M7/123 ,  H04M7/1235 ,  H04M7/125

Abstract: A bridge for linking a first communications network to a second communications network is taught. The bridge comprises a first bridge module linked to the first communications network and a second bridge module linked to the second communications network. The first bridge module is linked to the second bridge module by only an analog audio link and a General Purpose Input Output call negotiation signalling link such that generic digital data cannot effectively pass between the first communications network and the second communications network through the bridge elements. There is also provided a network terminal for connection to a network connected to the bridge and communications system comprising the bridge and first communications network.

Abstract translation: 教导了用于将第一通信网络链接到第二通信网络的桥接器。 桥接器包括链接到第一通信网络的第一桥模块和链接到第二通信网络的第二桥模块。 第一桥模块仅通过模拟音频链路和通用输入输出呼叫协商信令链路链接到第二桥模块，使得通用数字数据不能通过桥元件在第一通信网络和第二通信网络之间有效地通过。 还提供了一种网络终端，用于连接到连接到包括桥接器和第一通信网络的桥接器和通信系统的网络。

公开(公告)号：US20060225291A1

公开(公告)日：2006-10-12

申请号：US11398781

申请日：2006-04-05

Applicant: James Marks

Inventor： James Marks

IPC: B43L9/00

CPC classification number: B43L13/20

Abstract: A universal reusable template for cutting ends of cylindrical objects such as pipes, in preparation for joining the cylindrical objects to other cylindrical bodies. The template is formed as a flat flexible bendable sheet with magnetic properties allowing the sheet to be temporarily secured on a metal pipe. The front face of the sheet has reference indicia corresponding to contour lines of a desired cut to be made in the end of the cylindrical body. The cut lines represent most conventional types and sizes of lateral-type cuts, eccentric cuts and saddle-type cuts, with pipe diameters ranging from about 3″ to about 24″.

Abstract translation: 用于切割诸如管子的圆柱形物体的端部的通用可重用模板，以准备将圆柱形物体接合到其它圆柱体。 模板形成为具有磁性能的扁平柔性可弯曲片材，其允许片材临时固定在金属管上。 片材的正面具有对应于要在圆柱体的端部制成的期望切口的轮廓线的参考标记。 切割线表示大多数常规类型和尺寸的横向切割，偏心切割和鞍形切割，管直径范围为约3“至约24”。

公开(公告)号：US20060004601A1

公开(公告)日：2006-01-05

申请号：US11183418

申请日：2005-07-16

Applicant: James Marks

Inventor： James Marks

IPC: G06Q99/00

CPC classification number: G06Q10/10 ,  G06Q10/06311 ,  G06Q40/08 ,  G09B7/00

Abstract: A method and system, executed by a server, is disclosed for receiving questions from users regarding one or more topics and providing answers from a set of experts. Users can direct questions to one or more specific experts, who in turn can answer the questions, refer the questions to other experts, or both. The server automatically organizes and stores questions and answers in various fora dedicated to one or more topics, such as medical treatment for specific health conditions. Responses generated by the experts to the questions asked by the users may, in turn, be reviewed by peer review personnel. The system may be deployed at multiple network sites to provide a question and answer forum to users who visit different sites. Each network site in which the system is deployed may selectively decide whether to post at its network site the responses by experts and/or peer review personnel to questions originating from other network sites. In addition, each network site can selectively decide whether the responses by experts and/or peer review personnel to questions originating at their network site should be permitted to be posted on other network sites. Methods for funding a network site according to the present invention include the provision of advertising banners, receiving subscription payments or fees from user/subscribers for receiving expert opinions, and receiving payments from third party sponsors. Users who access the site may, in turn, receive benefits in exchange for soliciting such expert opinions.

Abstract translation: 公开了一种由服务器执行的方法和系统，用于从用户接收关于一个或多个主题的问题并从一组专家提供答案。 用户可以向一个或多个特定专家提出问题，反过来可以回答问题，将问题转交给其他专家或两者。 服务器自动组织和存储各种论坛中的问题和答案，专门针对一个或多个主题，如针对特定健康状况的医疗。 由专家对用户提出的问题产生的响应可以由同行评审人员进行审查。 该系统可以部署在多个网站，为访问不同站点的用户提供问答平台。 系统部署的每个网络站点可以选择性地决定是否在其网络站点发布专家和/或同行评审人员对来自其他网络站点的问题的响应。 此外，每个网络站点都可以选择性地决定是否允许专家和/或同行评审人员在其网站发起的问题的响应被发布在其他网站上。 根据本发明的为网站提供资金的方法包括提供广告横幅，从用户/订户接收订阅付款或收费以接收专家意见，以及从第三方赞助商接收付款。 进入该网站的用户反过来可以获得收益，以换取征求此类专家意见。