公开(公告)号：US20060247265A1

公开(公告)日：2006-11-02

申请号：US11116942

申请日：2005-04-28

Applicant: Timothy Clackson ,  Leonard Rozamus

Inventor： Timothy Clackson ,  Leonard Rozamus

IPC: A61K31/4745

CPC classification number: A61K31/4745

Abstract: Disclosed is the use of an mTOR inhibitor for treatment of certain eye disorders.

Abstract translation: 公开了使用mTOR抑制剂治疗某些眼睛疾病。

公开(公告)号：US20060194829A1

公开(公告)日：2006-08-31

申请号：US11312296

申请日：2005-12-20

Applicant: Timothy Clackson ,  Camille Bedrosian

Inventor： Timothy Clackson ,  Camille Bedrosian

IPC: A61K31/4745 ,  A61K31/4545 ,  A61K31/445

CPC classification number: A61K9/006 ,  A61K31/00 ,  A61K31/445 ,  A61K31/4545 ,  A61K31/4745

Abstract: Disclosed are compositions and methods for treating or preventing mucositis by administering to the patient an FKBP ligand prior to, during, or after treatments commonly associated with the development of mucositis such as certain chemotherapies, radiation therapies, or combinations thereof, but in particular, administration of an mTOR inhibitor such as rapamycin, AP23573, CC1779 or everolimus.

Abstract translation: 公开了用于治疗或预防粘膜炎的组合物和方法，其通过在通常与粘膜炎的发展相关的治疗之前，期间或之后给患者施用FKBP配体，例如某些化学疗法，放射疗法或其组合，但特别是给药 的mTOR抑制剂如雷帕霉素，AP23573，CC1779或依维莫司。

公开(公告)号：US06566073B1

公开(公告)日：2003-05-20

申请号：US09420819

申请日：1999-10-19

Applicant: Victor Rivera ,  Timothy Clackson ,  James Rothman

Inventor： Victor Rivera ,  Timothy Clackson ,  James Rothman

IPC: G01N3353

CPC classification number: C07K14/62 ,  A01K2217/05 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/811 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/04 ,  C07K2319/09 ,  C07K2319/32 ,  C07K2319/50 ,  C07K2319/60 ,  C07K2319/71 ,  C07K2319/715 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/625 ,  C12N2799/022

Abstract: Materials and methods involving conditional retention domains (CRDs) are disclosed. Also disclosed are fusion proteins containing CRDs and cells expressing such fusion proteins. In addition, the invention provides novel methods for producing target proteins in vivo using fusion proteins containing conditional retention domains and methods for identifying novel CRDs.

公开(公告)号：US20070292922A1

公开(公告)日：2007-12-20

申请号：US11729341

申请日：2007-03-28

Applicant: Jianmin Fang ,  Karin Jooss ,  Minh Nguyen ,  Thomas Harding ,  Timothy Clackson ,  Victor Rivera

Inventor： Jianmin Fang ,  Karin Jooss ,  Minh Nguyen ,  Thomas Harding ,  Timothy Clackson ,  Victor Rivera

IPC: C12P21/08 ,  C07K16/00 ,  C12N15/00 ,  C12N5/10

CPC classification number: C12N15/86 ,  A61K48/0066 ,  C07K14/005 ,  C07K16/2863 ,  C07K2319/50 ,  C12N2750/14143 ,  C12N2770/32122 ,  C12N2830/002

Abstract: Single AAV vector constructs for regulated expression of an immunoglobulin molecule or fragment thereof and methods of making and using the same are described. The AAV vectors comprise a regulated promoter operably linked to the coding sequence for a first and second immunoglobulin coding sequence, a sequence encoding a self-processing cleavage site between the coding sequence for the first and second immunoglobulin coding sequence and a additional proteolytic cleavage site, which provides a means to remove the self processing peptide sequence from an expressed immunoglobulin molecule or fragment thereof. The vector constructs find utility in enhanced production of biologically active immunoglobulins or fragments thereof in vitro and in vivo.

Abstract translation: 描述了免疫球蛋白分子或其片段的调节表达的单一AAV载体构建体及其制备和使用方法。 AAV载体包含与第一和第二免疫球蛋白编码序列的编码序列可操作地连接的调控启动子，编码第一和第二免疫球蛋白编码序列的编码序列与另外的蛋白水解切割位点之间的自加工切割位点的序列， 其提供了从表达的免疫球蛋白分子或其片段中除去自身加工肽序列的方法。 载体构建体在体外和体内发现在生物活性免疫球蛋白或其片段的增强生产中起作用。

公开(公告)号：US20070148774A1

公开(公告)日：2007-06-28

申请号：US11555519

申请日：2006-11-01

Applicant: John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

Inventor： John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

IPC: C12N15/86 ,  C12N15/74

CPC classification number: C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。