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    METHOD AND MEANS TO MODULATE PROGRAMMED CELL DEATH IN EUKARYOTIC CELLS
    11.
    发明申请
    METHOD AND MEANS TO MODULATE PROGRAMMED CELL DEATH IN EUKARYOTIC CELLS 有权
    调节细胞凋亡细胞死亡的方法和手段

    公开(公告)号：US20120164650A1

    公开(公告)日：2012-06-28

    申请号：US13244200

    申请日：2011-09-23

    申请人： Elena BABIYCHUK ,  Sergei Kushnir ,  Marc De Block

    发明人： Elena BABIYCHUK ,  Sergei Kushnir ,  Marc De Block

    IPC分类号： C12Q1/68 ,  C12N15/82 ,  G01N33/50

    CPC分类号： C12N15/8289 ,  C12N9/1077 ,  C12N15/8263 ,  Y10T436/143333

    摘要： The invention provides for the use of isolated polynucleotides encoding maize poly (ADP-ribose) polymerase (PARP) proteins to produce eukaryotic cells and organisms, particularly plant cells and plants, with modified programmed cell death. Eukaryotic cells and organisms particularly plant cells and plants, are provided wherein either in at least part of the cells, preferably selected cells, the programmed cell death (PCD) is provoked, or wherein, on the contrary, PCD of the cells or of at least part of the cells in an organism is inhibited, by modulation of the level or activity or PARP proteins in those cells.

    摘要翻译： 本发明提供编码玉米多聚（ADP-核糖）聚合酶（PARP）蛋白的分离的多核苷酸用于产生具有修饰的程序性细胞死亡的真核细胞和生物体，特别是植物细胞和植物的用途。 提供真核细胞和生物体，特别是植物细胞和植物，其中在至少部分细胞中，优选选择的细胞，程序性细胞死亡（PCD）被激发，或者相反地，细胞的PCD或 通过调节这些细胞中的水平或活性或PARP蛋白，抑制生物体中的最少部分细胞。

    Method and means to modulate programmed cell death in eukaryotic cells
    12.
    发明授权
    Method and means to modulate programmed cell death in eukaryotic cells 有权
    调节真核细胞程序性细胞死亡的方法和手段

    公开(公告)号：US08058510B2

    公开(公告)日：2011-11-15

    申请号：US11802343

    申请日：2007-05-22

    申请人： Elena Babiychuk ,  Sergei Kushnir ,  Marc De Block

    发明人： Elena Babiychuk ,  Sergei Kushnir ,  Marc De Block

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8289 ,  C12N9/1077 ,  C12N15/8263 ,  Y10T436/143333

    摘要： The invention provides for the use of isolated polynucleotides encoding maize poly (ADP-ribose) polymerase (PARP) proteins to produce eukaryotic cells and organisms, particularly plant cells and plants, with modified programmed cell death. Eukaryotic cells and organisms particularly plant cells and plants, are provided wherein either in at least part of the cells, preferably selected cells, the programmed cell death (PCD) is provoked, or wherein, on the contrary, PCD of the cells or of at least part of the cells in an organism is inhibited, by modulation of the level or activity or PARP proteins in those cells.

    摘要翻译： 本发明提供编码玉米多聚（ADP-核糖）聚合酶（PARP）蛋白的分离的多核苷酸用于产生具有修饰的程序性细胞死亡的真核细胞和生物体，特别是植物细胞和植物的用途。 提供真核细胞和生物体，特别是植物细胞和植物，其中在至少部分细胞中，优选选择的细胞，程序性细胞死亡（PCD）被激发，或者相反地，细胞的PCD或 通过调节这些细胞中的水平或活性或PARP蛋白，抑制生物体中的最少部分细胞。

    Methods and means for increasing the tolerance of plants to stress conditions
    14.
    发明申请
    Methods and means for increasing the tolerance of plants to stress conditions 有权
    增加植物对胁迫条件的耐受性的方法和手段

    公开(公告)号：US20060185038A1

    公开(公告)日：2006-08-17

    申请号：US10552552

    申请日：2004-04-09

    申请人： Marc De Block

    发明人： Marc De Block

    IPC分类号： A01H1/00 ,  C12N15/82

    CPC分类号： C12Y302/01143 ,  C12N9/0028 ,  C12N15/8271 ,  C12Y105/01011

    摘要： Methods and means are provided to increase the tolerance of plants to abiotic stress or adverse growing conditions, including drought, high light intensities, high temperatures, nutrient limitations and the like by reducing the activity of endogenous PARG proteins in plants.

    摘要翻译： 提供了方法和手段，以通过降低植物中内源性PARG蛋白的活性来增加植物对非生物胁迫或不利生长条件的耐受性，包括干旱，高光强度，高温，营养限制等。

    Genetic transformation using a PARP inhibitor
    15.
    发明授权
    Genetic transformation using a PARP inhibitor 失效
    使用PARP抑制剂的遗传转化

    公开(公告)号：US6074876A

    公开(公告)日：2000-06-13

    申请号：US817188

    申请日：1997-05-15

    申请人： Marc De Block

    发明人： Marc De Block

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/55 ,  C12N15/79 ,  C12N15/82 ,  G01N33/50 ,  A01H1/02 ,  C12N15/84

    CPC分类号： G01N33/5091 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8213 ,  C12N15/8289 ,  G01N33/5097

    摘要： The invention concerns a process for producing transgenic plant cells, which comprises: contacting a culture of plant cells with an inhibitor of poly-(ADP-ribose) polymerase, prior to transformation, for a period of time sufficient to reduce the response of the cultured cells to stress and to reduce their metabolism. The untransformed cells are then contacted with foreign DNA comprising at least one gene of interest under conditions in which the foreign DNA is taken up by the untransformed cells and the gene of interest is stably integrated in the nuclear genome of the untransformed cells to produce the transgenic cells. The transgenic plant cells are recovered from the culture.The invention further concerns a process for increasing the frequency of obtaining transgenic plant cells, via Agrobacterium-mediated transformation, which comprises: contacting a culture of plant cells with an inhibitor or poly(ADP-ribose) polymerase prior to transformation for a period of approximately 1 to 2 days or culturing transgenic plant cells after transfomation in a medium containing an inhibitor of poly (ADP-ribose) polymerase for a period of time of approximately 1 to 14 days.

    摘要翻译： PCT No.PCT / EP96 / 03366 Sec。 371日期1997年5月15日 102（e）日期1997年5月15日PCT提交1996年7月31日PCT公布。 出版物WO97 / 06267 日期1997年2月20日本发明涉及一种生产转基因植物细胞的方法，其包括：在转化之前将植物细胞培养物与聚（ADP-核糖）聚合酶抑制剂接触一段足以减少 培养细胞应激和减少其代谢的反应。 然后将未转化的细胞与包含至少一种目的基因的外源DNA在外源DNA被未转化细胞摄取的条件下接触，并且目的基因稳定整合到未转化细胞的核基因组中以产生转基因 细胞。 从培养物中回收转基因植物细胞。 本发明还涉及通过农杆菌介导的转化增加获得转基因植物细胞的频率的方法，其包括：在转化前将植物细胞的培养物与抑制剂或聚（ADP-核糖）聚合酶接触约一段时间 1至2天或在含有聚（ADP-核糖）聚合酶抑制剂的培养基中在转染后培养转基因植物细胞约1至14天的时间。

    Method and means for determining fitness in plants
    20.
    发明授权
    Method and means for determining fitness in plants 有权
    确定植物适应性的方法和手段

    公开(公告)号：US07951990B2

    公开(公告)日：2011-05-31

    申请号：US11451325

    申请日：2006-06-13

    申请人： Marc De Block

    发明人： Marc De Block

    IPC分类号： A01H4/00

    CPC分类号： G01N31/005 ,  A01H1/04

    摘要： The present invention provides methods and means for determining parent inbred plant lines with good combining ability, for determining good combinations of parent inbred plant lines capable of yielding hybrid lines with high heterosis, and further for determining the agronomical performance of different plant lines, which can be performed in vitro by determining the electron flow in the mitochondria under control and stress conditions.

    摘要翻译： 本发明提供用于确定具有良好组合能力的亲本近交植物品系的方法和手段，用于确定能够产生具有高杂种优势的杂交品系的亲本近交系的良好组合，并进一步确定不同植物品系的农学性能， 通过在对照和应激条件下测定线粒体中的电子流，在体外进行。