公开(公告)号：US09279130B2

公开(公告)日：2016-03-08

申请号：US13522506

申请日：2011-01-24

申请人： Marc De Block ,  Frank Meulewaeter ,  Vincent Bulone ,  Gea Guerriero

发明人： Marc De Block ,  Frank Meulewaeter ,  Vincent Bulone ,  Gea Guerriero

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/05 ,  C12N9/10

CPC分类号： C12N15/8246 ,  C12N9/1051 ,  C12Y204/01016

摘要： Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding a Saprolegnia monoica chitin synthase in cotton plants.

摘要翻译： 提供了用于修饰植物二次细胞壁的反应性的方法和手段，特别是在棉纤维中发现的棉花细胞壁中。 这可以通过在棉花植物中表达编码Saprolegnia monoica几丁质合酶的嵌合基因来方便地实现。

公开(公告)号：US08901369B2

公开(公告)日：2014-12-02

申请号：US13378536

申请日：2010-06-11

申请人： Marc De Block

发明人： Marc De Block

IPC分类号： A01H1/00 ,  A01H3/00

CPC分类号： A01H1/04 ,  A01H1/02 ,  A01H3/00 ,  A01H5/08 ,  A01H5/10 ,  G01N33/5097 ,  Y02A40/14

摘要： Methods are provided to select plants and populations of epigenetically fixed crop plants with improved yield.

摘要翻译： 提供方法来选择具有提高产量的表观遗传固定作物的植物和种群。

公开(公告)号：US08685736B2

公开(公告)日：2014-04-01

申请号：US13244200

申请日：2011-09-23

申请人： Elena Babiychuk ,  Kushnir Sergei ,  Marc De Block

发明人： Elena Babiychuk ,  Kushnir Sergei ,  Marc De Block

IPC分类号： C12N15/82 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/8289 ,  C12N9/1077 ,  C12N15/8263 ,  Y10T436/143333

摘要： The invention provides for the use of isolated polynucleotides encoding maize poly (ADP-ribose) polymerase (PARP) proteins to identify endogenous PARP encoding genes or cDNAs from a plant and to produce plant cells and plants with high vigor.

摘要翻译： 本发明提供了使用编码玉米聚（ADP-核糖）聚合酶（PARP）蛋白的分离的多核苷酸来鉴定来自植物的内源性PARP编码基因或cDNA并产生具有高活力的植物细胞和植物。

公开(公告)号：US20130312141A1

公开(公告)日：2013-11-21

申请号：US13942204

申请日：2013-07-15

申请人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

发明人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

IPC分类号： C12N15/82 ,  D02G3/02 ,  C12N9/10

CPC分类号： C12N15/8246 ,  C12N9/1051 ,  D02G3/02 ,  Y10T428/249921

摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

摘要翻译： 提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。 这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。

公开(公告)号：US20130191936A1

公开(公告)日：2013-07-25

申请号：US13876365

申请日：2011-09-26

申请人： Marc De Block ,  Matthew Hannah ,  Katrien Van Der Kelen ,  Frank Van Breusegem

发明人： Marc De Block ,  Matthew Hannah ,  Katrien Van Der Kelen ,  Frank Van Breusegem

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C12N15/82

摘要： Means and methods are provided to produce abiotic stress tolerant with improved yield based on the specific identification of a gene expression signature in said plants out of a population of said plants.

摘要翻译： 提供了基于在所述植物群体中所述植物中基因表达特征的特异性鉴定来提高产量的方法和方法以产生具有改善产量的非生物胁迫。

公开(公告)号：US20120030844A1

公开(公告)日：2012-02-02

申请号：US13187378

申请日：2011-07-20

申请人： Marc DE BLOCK ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

发明人： Marc DE BLOCK ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

IPC分类号： C08B15/00 ,  B32B5/02 ,  C12N15/54 ,  C12P19/04 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8246 ,  C12N9/1051 ,  D02G3/02 ,  Y10T428/249921

摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

摘要翻译： 提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。 这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。

公开(公告)号：US08008544B2

公开(公告)日：2011-08-30

申请号：US11993816

申请日：2006-06-19

申请人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

发明人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/11 ,  C12N15/31 ,  C12N15/56

CPC分类号： C12N15/8246 ,  C12N9/1051 ,  D02G3/02 ,  Y10T428/249921

摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

摘要翻译： 提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。 这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。

公开(公告)号：US20050014268A1

公开(公告)日：2005-01-20

申请号：US10849939

申请日：2004-05-21

申请人： Marc De Block

发明人： Marc De Block

IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/55 ,  C12N15/79 ,  C12N15/82 ,  G01N33/50 ,  C12N15/87

CPC分类号： G01N33/5091 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8213 ,  C12N15/8289 ,  G01N33/5097

摘要： The invention concerns a process for producing transgenic plant cells, which comprises: contacting a culture of plant cells with an inhibitor of poly-(ADP-ribose) polymerase, prior to transformation, for a period of time sufficient to reduce the response of the cultured cells to stress and to reduce their metabolism. The untransformed cells are then contacted with foreign DNA comprising at least one gene of interest under conditions in which the foreign DNA is taken up by the untransformed cells and the gene of interest is stably integrated in the nuclear genome of the untransformed cells to produce the transgenic cells. The transgenic plant cells are recovered from the culture. The invention further concerns a process for increasing the frequency of obtaining transgenic plant cells, via Agrobacterium-mediated transformation, which comprises: contacting a culture of plant cells with an inhibitor or poly(ADP-ribose) polymerase prior to transformation for a period of approximately 1 to 2 days or culturing transgenic plant cells after transformation in a medium containing an inhibitor of poly(ADP-ribose) polymerase for a period of time of approximately 1 to 14 days.

摘要翻译： 本发明涉及一种生产转基因植物细胞的方法，其包括：在转化之前将植物细胞培养物与多聚（ADP-核糖）聚合酶抑制剂接触一段足以减少培养物 细胞应激并减少其新陈代谢。 然后将未转化的细胞与包含至少一种目的基因的外源DNA在外源DNA被未转化细胞摄取的条件下接触，并且目的基因稳定整合到未转化细胞的核基因组中以产生转基因 细胞。 从培养物中回收转基因植物细胞。 本发明还涉及通过农杆菌介导的转化增加获得转基因植物细胞的频率的方法，其包括：在转化前将植物细胞的培养物与抑制剂或聚（ADP-核糖）聚合酶接触约一段时间 1至2天或在含有聚（ADP-核糖）聚合酶抑制剂的培养基中转化后培养转基因植物细胞约1至14天的时间。

公开(公告)号：US20130283482A1

公开(公告)日：2013-10-24

申请号：US13901948

申请日：2013-05-24

申请人： Marc De Block ,  Michael Metzlaff ,  Veronique Gossele

发明人： Marc De Block ,  Michael Metzlaff ,  Veronique Gossele

IPC分类号： C12N15/82

CPC分类号： C12N15/8271 ,  C12N9/1077 ,  C12N9/80 ,  C12N9/93

摘要： Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway from fungal or yeast like organisms other than Saccharomyces cereviseae, e.g., for overexpression in plants.

公开(公告)号：US08507755B2

公开(公告)日：2013-08-13

申请号：US13187378

申请日：2011-07-20

申请人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

发明人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

IPC分类号： C12N15/82 ,  C12N15/10 ,  C12N15/00 ,  A01H5/00

CPC分类号： C12N15/8246 ,  C12N9/1051 ,  D02G3/02 ,  Y10T428/249921

摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

摘要翻译： 提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。 这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。