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公开(公告)号:US20020164605A1
公开(公告)日:2002-11-07
申请号:US09966312
申请日:2001-09-28
申请人: Nanosphere, Inc.
发明人: Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号: C12Q001/68
CPC分类号: B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号:US20020160381A1
公开(公告)日:2002-10-31
申请号:US09975498
申请日:2001-10-11
申请人: Nanosphere, Inc.
发明人: Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas Andrew Taton
IPC分类号: C12Q001/68
CPC分类号: B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号:US20180071732A1
公开(公告)日:2018-03-15
申请号:US15819168
申请日:2017-11-21
申请人: Nanosphere, Inc.
发明人: Tom WESTBERG , Bruce RICHARDSON , Ryan GRISWOLD , Chris LEWIS
IPC分类号: B01L3/00
CPC分类号: B01L3/50853 , B01L2200/0689 , B01L2200/16 , B01L2300/042 , B01L2300/044 , B01L2300/046 , B01L2300/123
摘要: Disclosed arc cartridges and modules that may be utilized in diagnostic systems and methods. The cartridge includes a flexible seal that caps the cartridge. The flexible seal has an opening for a pipette tip, and the flexible seal is configured to create a sealed environment when a pipette tip is positioned in the opening of the flexible seal and when the pipette tip is moved in the X-axis, Y-axis, and Z-axis.
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公开(公告)号:US20040146917A1
公开(公告)日:2004-07-29
申请号:US10757780
申请日:2004-01-15
申请人: Nanosphere, Inc.
发明人: William Cork , Tim Patno , Mark Weber , Dave Morrow , Wesley Buckingham
IPC分类号: C12Q001/68 , C12M001/34
CPC分类号: G01N21/552 , G01N21/47 , G01N33/54346 , G01N33/54373 , G01N2015/0038
摘要: An apparatus and method for imaging metallic nanoparticles. The invention teaches an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact system without the need for complex motorized devices to move a camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.
摘要翻译: 用于金属纳米颗粒成像的装置和方法。 本发明教导了用于检测金胶体颗粒并且准确地向操作者报告的装置和方法。 该装置包括用于保持基板的基板保持器,处理器和存储器件,成像模块,照明模块,输入模块和输出模块。 该装置可以具有彼此靠近的固定衬底保持器和成像模块。 该设备提供了紧凑的系统,而不需要复杂的电动装置来使相机移动穿过基板。 此外,该装置和方法提供对基板上的点/孔的自动检测,基板上的点的自动定量,以及基于判定统计的斑点的自动解释。
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公开(公告)号:US20040110220A1
公开(公告)日:2004-06-10
申请号:US10716829
申请日:2003-11-18
申请人: Nanosphere, Inc.
发明人: Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton , Viswanadham Garimella , Zhi Li
IPC分类号: C12Q001/68
CPC分类号: B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/00 , C40B40/06 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2525/161 , C12Q2565/625 , C12Q2537/125
摘要: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号:US20040096856A1
公开(公告)日:2004-05-20
申请号:US10447073
申请日:2003-05-28
申请人: Nanosphere, Inc.
IPC分类号: B05D003/00 , C12Q001/68 , G01N033/53
CPC分类号: G01N33/552 , C03C17/30 , C12Q1/6834 , Y10T436/17 , Y10T436/172307 , Y10T436/20
摘要: A method for the efficient immobilization of silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces.
摘要翻译: 提供了一种将甲硅烷基化分子例如甲硅烷基化的寡核苷酸或蛋白质有效固定在未改性的表面如玻璃表面上的方法。 还提供了用于改变诸如玻璃表面的表面的化合物,装置和试剂盒。
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公开(公告)号:US20030148282A1
公开(公告)日:2003-08-07
申请号:US09976968
申请日:2001-10-12
申请人: Nanosphere, Inc.
发明人: Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号: C12Q001/68 , C07H021/04
CPC分类号: C07H21/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C07B2200/11 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号:US20020182611A1
公开(公告)日:2002-12-05
申请号:US09966491
申请日:2001-09-28
申请人: Nanosphere, Inc.
发明人: Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号: C12Q001/68
CPC分类号: B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing the nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号:US20020155462A1
公开(公告)日:2002-10-24
申请号:US09976577
申请日:2001-10-12
申请人: Nanosphere, Inc.
发明人: Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas Andrew Taton
IPC分类号: C12Q001/68
CPC分类号: B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号:US20020106461A1
公开(公告)日:2002-08-08
申请号:US10095585
申请日:2002-03-13
申请人: Nanosphere, Inc.
发明人: James D. Talton
IPC分类号: B05D007/00 , C23C014/30
CPC分类号: C23C14/223 , A61K9/5089 , C23C14/12 , C23C14/28
摘要: Methods of coating core materials by providing target materials and core materials; ablating the target materials to form ablated particulate target materials; and coating the core materials with said ablated particulate target materials; wherein the method is performed at a pressure of about 10 Torr or higher. Methods of coating particles with nanometer to multiple nanometer thick coatings in atmospheric pressure, and using pneumatic fluidization, are also provided.
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