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公开(公告)号：US20100273221A1
公开(公告)日：2010-10-28
申请号：US12834381
申请日：2010-07-12
申请人： Ryo Takeshita , Shinichi Sugimoto
发明人： Ryo Takeshita , Shinichi Sugimoto
摘要： A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
摘要翻译： 一种通过发酵生产碱性物质的方法，包括在包含在发酵罐中的液体培养基中培养具有生产碱性物质的能力的微生物，以在培养基中产生和积累碱性物质，其中硫酸盐和/或氯离子的量 用作碱性物质的抗衡离子的方法是通过在培养过程的至少一部分时间内将培养基中的总氨浓度调节到特定浓度范围内来减少。
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12.发明申请公开(公告)号：US20090258401A1
公开(公告)日：2009-10-15
申请号：US12137666
申请日：2008-06-12
申请人： Mayu Iyo , Ryo Takeshita , Shinichi Sugimoto
发明人： Mayu Iyo , Ryo Takeshita , Shinichi Sugimoto
摘要： L-amino acid is produced by culturing a bacterium belonging to the Enterobacteriaceae family which has L-amino acid-producing ability and is modified so that expression of the nhaA gene, nhaB gene, nhaR gene, chaA gene, mdfA gene, or combinations thereof is enhanced.
摘要翻译： L-氨基酸是通过培养具有L-氨基酸生产能力的属于肠杆菌科的细菌产生的，并被修饰以使nhaA基因，nhaB基因，nhaR基因，chaA基因，mdfA基因或其组合的表达 增强了。
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13.发明申请公开(公告)号：US20090215130A1
公开(公告)日：2009-08-27
申请号：US12179988
申请日：2008-07-25
申请人： Mayu Iyo , Ryo Takeshita , Shinichi Sugimoto
发明人： Mayu Iyo , Ryo Takeshita , Shinichi Sugimoto
IPC分类号： C12P13/24 , C12N1/21 , C12P13/04 , C12P13/08 , C12P13/06 , C12P13/22 , C12P13/12 , C12P13/14
CPC分类号： C12P13/08
摘要： An L-amino acid is produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the activity of an iron transporter is increased by enhancing expression of one or more genes of the following genes: tonB gene, fepA gene, and fecA.
摘要翻译： L-氨基酸是通过培养属于肠杆菌科的L-氨基酸产生细菌而经过修饰的，通过增强以下基因的一个或多个基因的表达来增加铁转运蛋白的活性 ：tonB基因，fepA基因和fecA。
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公开(公告)号：US07163810B2
公开(公告)日：2007-01-16
申请号：US10234329
申请日：2002-09-05
申请人： Hisashi Yasueda , Ryo Takeshita
发明人： Hisashi Yasueda , Ryo Takeshita
CPC分类号： C12N9/0004 , C12P13/08
摘要： In a method for producing a target substance by using a microorganism comprising culturing a microorganism having an ability to produce the target substance in a medium to produce and accumulate the target substance in the medium or cells of the microorganism and collecting the target substance from the medium or the cells of the microorganism, there are used, as the microorganism, a microorganism to which a methanol dehydrogenase gene is introduced, of which activities of hexulose phosphate synthase and phosphohexuloisomerase are enhanced and which is modified so that an ability to utilize methanol should be imparted or enhanced, and there is used a medium containing methanol as a carbon source.
摘要翻译： 在通过使用微生物制造目标物质的方法中，包括在培养基中培养具有产生目标物质的能力的微生物，以在所述微生物的培养基或细胞中产生并积累所述目标物质并从所述培养基中收集所述目标物质 或微生物的细胞，使用引入了甲醇脱氢酶基因的微生物作为微生物，其中己糖磷酸合成酶和磷酸己糖异构酶的活性被增强，并且被修饰，使得使用甲醇的能力应该是 赋予或增强，并且使用含有甲醇作为碳源的介质。
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公开(公告)号：US07160704B2
公开(公告)日：2007-01-09
申请号：US10792647
申请日：2004-03-04
申请人： Ryo Takeshita , Hisashi Yasueda
发明人： Ryo Takeshita , Hisashi Yasueda
CPC分类号： C12N9/0006 , C12N9/1051 , C12N9/90 , C12P13/08
摘要： The present invention discloses a method for producing a target substance using a coryneform bacterium comprising culturing a coryneform bacterium having an ability to produce the target substance in a medium, resulting in accumulation of the target substance in the medium or cells of the bacterium, and collecting the target substance from the medium or the cells of the bacterium. Also disclosed is a coryneform bacterium which is introduced with a methanol dehydrogenase gene and which has enhanced activities of hexulose phosphate synthase and phosphohexuloisomerase, and to which an ability to utilize methanol is imparted or which has enhanced ability to utilize methanol, and the medium contains methanol as a carbon source.
摘要翻译： 本发明公开了一种使用棒状细菌生产目标物质的方法，包括培养在培养基中具有产生目标物质的能力的棒状细菌，导致目标物质在细菌的培养基或细胞中的积聚，并收集 来自培养基的目标物质或细菌的细胞。 还公开了一种引入了甲醇脱氢酶基因并且具有增强的己糖磷酸合成酶和磷酸己糖异构酶的活性的棒状细菌，并且其赋予使用甲醇的能力或具有增强的利用甲醇的能力，并且所述培养基含有甲醇 作为碳源。
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公开(公告)号：US20050176121A1
公开(公告)日：2005-08-11
申请号：US10791853
申请日：2004-03-04
申请人： Ryo Takeshita , Hisashi Yasueda , Yoshiya Gunji
发明人： Ryo Takeshita , Hisashi Yasueda , Yoshiya Gunji
CPC分类号： C12P7/04
摘要： The present invention describes a recombinant of a microorganism that does not inherently utilize an alkane, and an alcohol, whereby the recombinant has acquired an ability to convert the alkane into the alcohol due to transformation with a DNA encoding a methane oxygenase. The present invention describes a method for producing alcohol by culturing the recombinant, and allowing the obtained culture, cells isolated from the culture or processed product of the cells to exist with the alkane to produce the alcohol.
摘要翻译： 本发明描述了一种不固有地利用烷烃和醇的微生物重组体，由此由于用编码甲烷加氧酶的DNA转化，重组体已经获得了将烷烃转化为醇的能力。 本发明描述了通过培养重组体来制备醇的方法，并且使所得培养物从细胞的培养物或加工产物中分离的细胞与烷烃一起存在以产生醇。
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公开(公告)号：US08198053B2
公开(公告)日：2012-06-12
申请号：US12834381
申请日：2010-07-12
申请人： Ryo Takeshita , Shinichi Sugimoto
发明人： Ryo Takeshita , Shinichi Sugimoto
摘要： A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
摘要翻译： 一种通过发酵生产碱性物质的方法，包括在包含在发酵罐中的液体培养基中培养具有生产碱性物质的能力的微生物，以在培养基中产生和积累碱性物质，其中硫酸盐和/或氯离子的量 用作碱性物质的抗衡离子的方法是通过在培养过程的至少一部分时间内将培养基中的总氨浓度调节到特定浓度范围内来减少。
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