公开(公告)号：US20200057289A1

公开(公告)日：2020-02-20

申请号：US16663182

申请日：2019-10-24

Applicant: ThinkCyte, Inc. ,  The University of Tokyo

Inventor： Masashi UGAWA ,  Yoko KAWAMURA ,  Sadao OTA

IPC: G02B21/00 ,  G02B21/18 ,  G02B21/04 ,  G01N15/14

Abstract: An imaging flow cytometer includes at least one flow channel through which an observation target flows, a light source which irradiates the flow channel with sheet-like excitation light, an imaging unit which images a specific cross-section of the observation target by imaging fluorescence from the observation target having passed through a position irradiated with the excitation light, and a three-dimensional image generation unit which generates a three-dimensional image of the observation target as a captured image on the basis of a plurality of captured images obtained by cross-sectional imaging by the imaging unit.

公开(公告)号：US20230348893A1

公开(公告)日：2023-11-02

申请号：US18186118

申请日：2023-03-17

Applicant: ThinkCyte, Inc. ,  The University of Tokyo ,  RIKEN

Inventor： Asako TSUBOUCHI ,  Sadao OTA ,  Fumiko KAWASAKI

IPC: C12N15/10

CPC classification number: C12N15/1055 ,  C12N15/1065 ,  C12N15/1089 ,  C40B30/04

Abstract: The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.

公开(公告)号：US11668696B2

公开(公告)日：2023-06-06

申请号：US17706962

申请日：2022-03-29

Applicant: RIKEN ,  ThinkCyte, Inc.

Inventor： Issei Sato ,  Masahiro Kazama ,  Masashi Ugawa ,  Hiroaki Adachi ,  Fumiya Shimada

IPC: G06V10/88 ,  G01N15/14 ,  G01N33/483 ,  G06V10/50 ,  G06V20/69 ,  G06F18/2115 ,  G06F18/21 ,  G06N3/045

CPC classification number: G06V10/88 ,  G01N15/1429 ,  G01N33/4833 ,  G06F18/2115 ,  G06F18/2163 ,  G06N3/045 ,  G06V10/507 ,  G06V20/695 ,  G06V20/698

Abstract: A mask structure optimization device includes a classification target image size acquisition unit that is configured to acquire a size of a classification target image which is an image including a classification target, a mask size setting unit that is configured to set a size of a mask applied to the classification target image, a brightness detection unit that is configured to detect a brightness of each pixel within the classification target image at a position on an opposite side of the mask from the classification target image, a sum total brightness calculation unit that is configured to calculate the sum total brightness of the each pixel within the classification target image detected by the brightness detection unit, an initial value setting unit that is configured to set an initial value for a mask pattern of the mask, and a movement unit that is configured to relatively move the mask with respect to the classification target image. The sum total brightness calculation unit is configured to calculate the sum total brightness of the each pixel within the classification target image every time the movement unit relatively moves the mask by a predetermined movement amount. The mask structure optimization device further includes a mask pattern optimization unit that is configured to optimize the mask pattern of the mask on the basis of the sum total brightness.

公开(公告)号：US20230012588A1

公开(公告)日：2023-01-19

申请号：US17935707

申请日：2022-09-27

Applicant: ThinkCyte, Inc. ,  The University of Tokyo

Inventor： Toru Imai ,  Sadao Ota

IPC: G01N15/14

Abstract: An observation device includes an illumination optical system and an observation optical system. The illumination optical system includes a light source and an aperture member. The observation optical system includes an objective lens, an optical structure, and a detector. The optical structure is disposed at a first position which is the position conjugate with the aperture member. The optical structure includes a blocking portion that blocks light and a transmitting portion that transmits light, the blocking portion having a shape including the shape of an image of an aperture of the aperture member which is formed on the optical structure. The detector detects dark-field light passing through the optical structure.

公开(公告)号：US20200150022A1

公开(公告)日：2020-05-14

申请号：US16610481

申请日：2018-05-02

Applicant: THINKCYTE, INC. ,  The University of Tokyo

Inventor： Masashi UGAWA ,  Yoko KAWAMURA ,  Sadao OTA

IPC: G01N15/14 ,  G01N33/483 ,  C12Q1/02 ,  G01N15/10

Abstract: A cell evaluation system includes physical measurement unit, a database, and evaluation unit. The evaluation unit refers to a relevance stored in the database, searches reference measurement information based on measurement information of a cell newly measured via the physical measurement unit, and evaluates the cell with biological measurement information associated with the searched reference measurement information.

公开(公告)号：US20200027020A1

公开(公告)日：2020-01-23

申请号：US16584535

申请日：2019-09-26

Applicant: ThinkCyte, Inc. ,  The University of Tokyo

Inventor： Ryosuke KAMESAWA ,  Sadao OTA

IPC: G06N20/00

Abstract: A learning result output apparatus includes a machine learning unit that performs machine learning on at least one of attributes of a learning target, with a degree of the attribute as an evaluation axis, on the basis of morphological information indicating a shape of the learning target, and a graph information generation unit that generates graph information indicating a graph representing a learning result obtained by performing machine learning in the machine learning unit, with the evaluation axis as an axis, on the basis of a learning model indicating the learning result.

公开(公告)号：US11643649B2

公开(公告)日：2023-05-09

申请号：US17770569

申请日：2021-01-12

Applicant: ThinkCyte, Inc. ,  The University of Tokyo ,  RIKEN

Inventor： Asako Tsubouchi ,  Sadao Ota ,  Fumiko Kawasaki

IPC: C40B30/04 ,  C12N15/10

CPC classification number: C12N15/1055 ,  C12N15/1065 ,  C12N15/1089 ,  C40B30/04

Abstract: The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.

公开(公告)号：US11630293B2

公开(公告)日：2023-04-18

申请号：US16663182

申请日：2019-10-24

Applicant: ThinkCyte, Inc. ,  The University of Tokyo

Inventor： Masashi Ugawa ,  Yoko Kawamura ,  Sadao Ota

IPC: G02B21/00 ,  G01N15/14 ,  G02B21/04 ,  G02B21/18

Abstract: An imaging flow cytometer includes at least one flow channel through which an observation target flows, a light source which irradiates the flow channel with sheet-like excitation light, an imaging unit which images a specific cross-section of the observation target by imaging fluorescence from the observation target having passed through a position irradiated with the excitation light, and a three-dimensional image generation unit which generates a three-dimensional image of the observation target as a captured image on the basis of a plurality of captured images obtained by cross-sectional imaging by the imaging unit.

公开(公告)号：US20230090631A1

公开(公告)日：2023-03-23

申请号：US17935696

申请日：2022-09-27

Applicant: ThinkCyte, Inc. ,  The University of Tokyo

Inventor： Toru Imai ,  Keisuke Toda ,  Sadao Ota

IPC: G01N15/14

Abstract: This flow cytometer includes a flow path through which an observation object flows with a fluid; an optical illumination system including a spatial optical modulation device, and a first optical element; and an optical detection system including a first light detector, wherein the optical illumination system further includes a first spatial filter disposed in a first optical path between a light source and an image position of light imaged in the flow path by the first optical element and having a first region which hinders traveling of light emitted from the light source towards the observation object, the optical detection system further includes a second light detector disposed in a second optical path between the first light detector and the image position and having a second region which directs the light modulated by the observation object towards the first light detector, and the position of the first region and the position of the second region are in a substantially optically conjugate relationship.

公开(公告)号：US20220317020A1

公开(公告)日：2022-10-06

申请号：US17847478

申请日：2022-06-23

Applicant: ThinkCyte, Inc.

Inventor： Keiji Nakagawa

IPC: G01N15/14

Abstract: A method of evaluating performance of a flow cytometer configured to use a combination of two or more types of calibration particles having different morphologies from each other, includes a first classification step of classifying the calibration particles from each other based on a first optical characteristic by the flow cytometer which is an evaluation target, a second classification step of classifying the calibration particles from each other based on a second optical characteristic which is classifiable at a spatial resolution lower than a spatial resolution at which the first optical characteristic is classified, and the evaluation step of evaluating one or both of particle classification performance and a resolution of the flow cytometer based on a first classification result assessed in the first classification step and a second classification result assessed in the second classification step.