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公开(公告)号：US11314994B2
公开(公告)日：2022-04-26
申请号：US16965311
申请日：2019-01-30
申请人： RIKEN , ThinkCyte, Inc.
发明人： Issei Sato , Masahiro Kazama , Masashi Ugawa , Hiroaki Adachi , Fumiya Shimada
摘要： A mask structure optimization device includes a classification target image size acquisition unit that is configured to acquire a size of a classification target image which is an image including a classification target, a mask size setting unit that is configured to set a size of a mask applied to the classification target image, a brightness detection unit that is configured to detect a brightness of each pixel within the classification target image at a position on an opposite side of the mask from the classification target image, a sum total brightness calculation unit that is configured to calculate the sum total brightness of the each pixel within the classification target image detected by the brightness detection unit, an initial value setting unit that is configured to set an initial value for a mask pattern of the mask, and a movement unit that is configured to relatively move the mask with respect to the classification target image. The sum total brightness calculation unit is configured to calculate the sum total brightness of the each pixel within the classification target image every time the movement unit relatively moves the mask by a predetermined movement amount. The mask structure optimization device further includes a mask pattern optimization unit that is configured to optimize the mask pattern of the mask on the basis of the sum total brightness.
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公开(公告)号：US11668696B2
公开(公告)日：2023-06-06
申请号：US17706962
申请日：2022-03-29
申请人： RIKEN , ThinkCyte, Inc.
发明人： Issei Sato , Masahiro Kazama , Masashi Ugawa , Hiroaki Adachi , Fumiya Shimada
IPC分类号： G06V10/88 , G01N15/14 , G01N33/483 , G06V10/50 , G06V20/69 , G06F18/2115 , G06F18/21 , G06N3/045
CPC分类号： G06V10/88 , G01N15/1429 , G01N33/4833 , G06F18/2115 , G06F18/2163 , G06N3/045 , G06V10/507 , G06V20/695 , G06V20/698
摘要： A mask structure optimization device includes a classification target image size acquisition unit that is configured to acquire a size of a classification target image which is an image including a classification target, a mask size setting unit that is configured to set a size of a mask applied to the classification target image, a brightness detection unit that is configured to detect a brightness of each pixel within the classification target image at a position on an opposite side of the mask from the classification target image, a sum total brightness calculation unit that is configured to calculate the sum total brightness of the each pixel within the classification target image detected by the brightness detection unit, an initial value setting unit that is configured to set an initial value for a mask pattern of the mask, and a movement unit that is configured to relatively move the mask with respect to the classification target image. The sum total brightness calculation unit is configured to calculate the sum total brightness of the each pixel within the classification target image every time the movement unit relatively moves the mask by a predetermined movement amount. The mask structure optimization device further includes a mask pattern optimization unit that is configured to optimize the mask pattern of the mask on the basis of the sum total brightness.
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公开(公告)号：US11630293B2
公开(公告)日：2023-04-18
申请号：US16663182
申请日：2019-10-24
发明人： Masashi Ugawa , Yoko Kawamura , Sadao Ota
摘要： An imaging flow cytometer includes at least one flow channel through which an observation target flows, a light source which irradiates the flow channel with sheet-like excitation light, an imaging unit which images a specific cross-section of the observation target by imaging fluorescence from the observation target having passed through a position irradiated with the excitation light, and a three-dimensional image generation unit which generates a three-dimensional image of the observation target as a captured image on the basis of a plurality of captured images obtained by cross-sectional imaging by the imaging unit.
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公开(公告)号：US11598712B2
公开(公告)日：2023-03-07
申请号：US16610481
申请日：2018-05-02
发明人： Masashi Ugawa , Yoko Kawamura , Sadao Ota
摘要： A cell evaluation system includes physical measurement unit, a database, and evaluation unit. The evaluation unit refers to a relevance stored in the database, searches reference measurement information based on measurement information of a cell newly measured via the physical measurement unit, and evaluates the cell with biological measurement information associated with the searched reference measurement information.
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公开(公告)号：US20210190669A1
公开(公告)日：2021-06-24
申请号：US17115657
申请日：2020-12-08
发明人： Sadao Ota , Ryoichi Horisaki , Yoko Kawamura , Masashi Ugawa , Issei Sato
IPC分类号： G01N15/14
摘要： The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence “imaging” cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.
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公开(公告)号：US11788948B2
公开(公告)日：2023-10-17
申请号：US17115657
申请日：2020-12-08
发明人： Sadao Ota , Ryoichi Horisaki , Yoko Kawamura , Masashi Ugawa , Issei Sato
CPC分类号： G01N15/1434 , G01N15/1459 , G01N2015/1006 , G01N2015/145 , G01N2015/149 , G01N2015/1497
摘要： The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence “imaging” cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.
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