公开(公告)号：US20250163501A1

公开(公告)日：2025-05-22

申请号：US18834805

申请日：2023-01-31

Applicant: 10x Genomics, Inc.

Inventor： Hardeep Pal Singh ,  David Sukovich ,  Augusto Manuel Tentori ,  Hanyoup Kim

IPC: C12Q1/6841 ,  C12Q1/37

Abstract: Provided herein are methods of analyzing an analyte in a fixed biological sample on a first substrate that has been stored for a long period of time, the method comprising: (a) hybridizing a first probe and a second probe to the analyte of the fixed biological sample; (b) coupling the first probe and the second probe, thereby generating a connected probe; (c) aligning the first substrate with a second substrate comprising an array, wherein the array comprises a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises: (i) a spatial barcode and (ii) a capture domain; (d) when the biological sample is aligned with at least a portion of the array, (i) releasing the connected probe from the analyte and (ii) migrating the connected probe from the biological sample to the array; and (e) hybridizing the connected probe to the capture domain.

公开(公告)号：US11959076B2

公开(公告)日：2024-04-16

申请号：US18448725

申请日：2023-08-11

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US20230332138A1

公开(公告)日：2023-10-19

申请号：US18140312

申请日：2023-04-27

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori ,  Lauren Gutgesell ,  Janine Hensel ,  Seayar Mohabbat

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US20230113230A1

公开(公告)日：2023-04-13

申请号：US17976422

申请日：2022-10-28

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori ,  Lauren Gutgesell ,  Janine Hensel ,  Seayar Mohabbat

IPC: C12N15/10

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US11584954B2

公开(公告)日：2023-02-21

申请号：US16855177

申请日：2020-04-22

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila Belhocine ,  Josephine Lee ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Andrew D. Price ,  Jerald Sapida ,  David Sukovich ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12Q1/6806

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20220356464A1

公开(公告)日：2022-11-10

申请号：US17871525

申请日：2022-07-22

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12N15/10

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US20240141327A1

公开(公告)日：2024-05-02

申请号：US18409174

申请日：2024-01-10

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori ,  Lauren Gutgesell ,  Janine Hensel ,  Seayar Mohabbat

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US11970739B2

公开(公告)日：2024-04-30

申请号：US18348063

申请日：2023-07-06

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Joseph Francis Shuga ,  Patrick Roelli ,  Denise Cheung ,  Alexander Hermes ,  Yifeng Yin ,  Naishitha Anaparthy ,  Ryo Hatori ,  Marlon Stoeckius ,  Rapolas Spalinskas ,  Anna-Maria Katsori ,  Cedric Uytingco ,  Mesruh Turkekul ,  David Sukovich ,  Christina Galonska ,  Aleksandra Jurek ,  Octavian Marian Bloju

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6837

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

公开(公告)号：US11873482B2

公开(公告)日：2024-01-16

申请号：US18140312

申请日：2023-04-27

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori ,  Lauren Gutgesell ,  Janine Hensel ,  Seayar Mohabbat

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US20240011090A1

公开(公告)日：2024-01-11

申请号：US18348063

申请日：2023-07-06

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Joseph Francis Shuga ,  Patrick Roelli ,  Denise Cheung ,  Alexander Hermes ,  Yifeng Yin ,  Naishitha Anaparthy ,  Ryo Hatori ,  Marlon Stoeckius ,  Rapolas Spalinskas ,  Anna-Maria Katsori ,  Cedric Uytingco ,  Mesruh Turkekul ,  David Sukovich ,  Christina Galonska ,  Aleksandra Jurek ,  Octavian Marian Bloju

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6837

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.