公开(公告)号：US20180272347A1

公开(公告)日：2018-09-27

申请号：US15762654

申请日：2016-09-23

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

发明人： Frederic ZENHAUSERN ,  Wooseok JUNG

IPC分类号： B01L3/00 ,  F16K99/00

CPC分类号： B01L3/502738 ,  B01L7/525 ,  B01L2200/0605 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2400/0633 ,  B01L2400/0655 ,  B01L2400/0661 ,  F16K99/0015 ,  F16K99/0044 ,  F16K2099/0084

摘要： Provided herein are methods and devices for controlled metering of a volume of fluid into a bioreactor chamber by use of a thermally-activated actuator that provides simultaneous temperature control and fluidic valve capability. The device may be an elastomeric valve assembly comprising: a thermally-activated actuator, a microfluidic cartridge and an elastomeric membrane operably connected to the thermally-activated actuator. Upon thermal activation of the thermally-activated actuator the elastomeric contact surface is forced into a lumen of the conduit of the microfluidic cartridge to fluidically seal the conduit, while the thermally-activated actuator is in thermal contact with a bioreactor chamber.

公开(公告)号：US20170045427A1

公开(公告)日：2017-02-16

申请号：US15119098

申请日：2015-02-20

申请人： The Arizona Board of Regents On Behalf Of The University of Arizona

发明人： Frederic ZENHAUSERN ,  Jian GU

IPC分类号： G01N1/28 ,  G01N21/84 ,  G01B11/06 ,  G06T3/40 ,  G01N15/14 ,  H04N5/225 ,  H04N5/76 ,  G01B11/10 ,  G01B11/24

CPC分类号： G01N1/2813 ,  G01B11/0616 ,  G01B11/105 ,  G01B11/2441 ,  G01N15/1468 ,  G01N15/1475 ,  G01N21/84 ,  G01N2015/0065 ,  G01N2015/1006 ,  G06T3/4076 ,  H04N5/2256 ,  H04N5/76

摘要： Provided herein are methods and related systems for controlling droplet spreading on a surface, including droplets in which a biological component is suspended. A biological solution is provided as a droplet to a surface. Interference fringes generated by the droplet on the surface are imaged, wherein the imaging is over a time course during which the droplet spreads on the surface. A droplet parameter is determined from the imaging step and a process parameter controlled to obtain an interference fringe pattern corresponding to a desired droplet parameter. In this manner, well-controlled droplet spreading is achieved, which is important in a range of applications, including assays that rely on good metaphase spreading.

摘要翻译： 本文提供了用于控制表面上的液滴扩散的方法和相关系统，包括其中悬浮生物组分的液滴。 将生物溶液作为液滴提供给表面。 由表面上的液滴产生的干涉条纹被成像，其中成像在液滴在表面上扩展的时间过程之上。 从成像步骤确定液滴参数，并且控制工艺参数以获得对应于所需液滴参数的干涉条纹图案。 以这种方式，实现了良好控制的液滴扩散，这在一系列应用中是重要的，包括依赖于良好中期扩散的测定。