公开(公告)号：US20210275980A1

公开(公告)日：2021-09-09

申请号：US16838762

申请日：2020-04-02

Applicant: Altria Client Services LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Dongmei XU ,  James Strickland

IPC: B01F13/08 ,  G01N1/38 ,  B01F15/02 ,  B01L3/00 ,  B01F13/00 ,  H02N11/00

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US20250011803A1

公开(公告)日：2025-01-09

申请号：US18896070

申请日：2024-09-25

Applicant: Altria Client Services LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Dongmei XU ,  James Strickland

IPC: C12N15/82 ,  A01H4/00 ,  A24B13/00 ,  A24B15/10 ,  A24B15/24 ,  B01F33/45 ,  B01F33/452 ,  B01F33/501 ,  B01F35/75 ,  B01L3/00 ,  C07K14/415 ,  C12N9/04 ,  G01N1/38 ,  G01N35/00 ,  G01N35/10 ,  H02N11/00

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US20240067977A1

公开(公告)日：2024-02-29

申请号：US17766786

申请日：2020-10-09

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Rajanikanth GOVINDARAJULU ,  Nazmul BHUIYAN ,  Raija PAYYAVULA ,  Yanxin SHEN ,  Dongmei XU

IPC: C12N15/82 ,  C07K14/415 ,  C12N15/113

CPC classification number: C12N15/8243 ,  C07K14/415 ,  C12N15/1137

Abstract: The present disclosure provides composition and methods for improving leaf quality of low-alkaloid tobacco plants. Also provided are the identification and genetic engineering of target genes (Leaf Quality Genes) for producing tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US20240035042A1

公开(公告)日：2024-02-01

申请号：US18476495

申请日：2023-09-28

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Dongmei XU ,  Chengalrayan KUDITHIPUDI ,  Yanxin SHEN ,  Jaemo YANG ,  Jesse FREDERICK ,  James STRICKLAND

IPC: C12N15/82 ,  C07K14/415 ,  A24B13/00 ,  A24B15/10

CPC classification number: C12N15/8261 ,  C07K14/415 ,  A24B13/00 ,  A24B15/10 ,  C12N15/827 ,  Y02A40/146

Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US20220142228A1

公开(公告)日：2022-05-12

申请号：US17587441

申请日：2022-01-28

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Jesse FREDERICK ,  Chengalrayan KUDITHIPUDI ,  Dongmei XU

IPC: A24B15/20 ,  C12N15/82 ,  C12N9/88 ,  A24B3/12 ,  C12N15/10 ,  C12N15/66

Abstract: This disclosure provides a number of sequences involved in nitrogen utilization, methods of using such sequences, tobacco plants carrying modifications to such sequences, tobacco plants transgenic for such sequences, and tobacco products made from such plants.

公开(公告)号：US20200071717A1

公开(公告)日：2020-03-05

申请号：US16674838

申请日：2019-11-05

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Alec J. HAYES ,  Marcos Fernando de GODOY LUSSO ,  Jerry Whit MORRIS

IPC: C12N15/82 ,  A24D3/04 ,  A24D1/00 ,  A24B13/00 ,  A24F47/00

Abstract: This disclosure provides tobacco plants containing a PMT RNAi and tobacco plants having a mutation in PMT, and methods of making and using such plants.

公开(公告)号：US20200060136A1

公开(公告)日：2020-02-27

申请号：US16529307

申请日：2019-08-01

Applicant: Altria Client Services LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Rutger VAN DER HOEVEN ,  Ndjido KANE

IPC: A01H5/12 ,  A01H1/06 ,  A24B13/00 ,  A24D1/00

Abstract: A process for producing a reduced tobacco-specific nitrosamine (TSNA) tobacco plant comprising reducing and/or eliminating anatabine biosynthesis in wild-type tobacco plant. In addition, use of such plants for discovery of molecular markers that are closely linked with genes required for anatabine biosynthesis and for discovery of genes required for anatabine biosynthesis. In addition, a smoking composition, a smoking article and a smokeless tobacco oral delivery product contain the tobacco material.

公开(公告)号：US20240376488A1

公开(公告)日：2024-11-14

申请号：US18782700

申请日：2024-07-24

Applicant: Altria Client Services LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Dongmei XU ,  James A. STRICKLAND ,  Marcos Fernando de Godoy Lusso

IPC: C12N15/82

Abstract: The present disclosure provides approaches for reducing nicotine. Also provided are tobacco plants with decreased tobacco-specific nitrosamines (TSNAs). The present disclosure further provides modified tobacco plants with low nicotine and increased antioxidant capacity. Further provided are tobacco plants or material with low nicotine and low TSNAs. Also provided is cured tobacco material of the tobacco plants provided herein and tobacco products comprising this cured tobacco material.

公开(公告)号：US20240229054A9

公开(公告)日：2024-07-11

申请号：US18365270

申请日：2023-08-04

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Rajanikanth GOVINDARAJULU ,  Raja S. PAYYAVULA

IPC: C12N15/82 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415

CPC classification number: C12N15/8243 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415

Abstract: The present disclosure relates to tobacco plants, plant parts, seeds, compositions, and methods related to modulating the expression of novel genes in tobacco to regulate alkaloid levels.

公开(公告)号：US20240218386A1

公开(公告)日：2024-07-04

申请号：US17781797

申请日：2020-12-02

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Chengalrayan KUDITHIPUDI ,  Yanxin SHEN ,  Dongmei XU

IPC: C12N15/82 ,  A24B13/00 ,  A24B15/00

CPC classification number: C12N15/8243 ,  A24B13/00 ,  A24B15/00

Abstract: The present disclosure provides composition and methods for manipulating alkaloid or nicotine levels in tobacco plants. Also provided are the identification and genetic engineering of target genes (e.g., arginine decarboxylase (ADC), aspartate oxidase (AO), or ornithine decarboxylase (ODC)) for producing tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.