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    Droplet tagging contiguity preserved tagmented DNA
    13.
    发明授权
    Droplet tagging contiguity preserved tagmented DNA 有权

    公开(公告)号：US11021738B2

    公开(公告)日：2021-06-01

    申请号：US15847408

    申请日：2017-12-19

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Ronald Lebofsky ,  Jeremy Agresti

    IPC: C12P19/34 ,  C12Q1/6809 ,  G16B20/00 ,  C12Q1/6869

    Abstract: A method of maintaining contiguity in chromosomal DNA following treatment with a tagmentase. Conditions are selected such that the tagmentase does not release from the DNA, and thus forms a bridge linking DNA segments that have the same relationship (haplotype) as occurred in the genomic DNA. Thus the tagmentase step can occur in bulk (before partitions are formed). The resulting tagmentase-bridged DNA segments can be added to partitions maintaining the bridged segments until they are introduced into different partitions. Once in partitions, the contiguous DNA segments can be barcoded with a partition-specific barcode, thereby allowing for later identification of contiguous DNA after sequencing in bulk (after partitions contents are merged).

    Use of gel beads to control droplet dispersion
    19.
    发明授权
    Use of gel beads to control droplet dispersion 有权

    公开(公告)号：US10233487B2

    公开(公告)日：2019-03-19

    申请号：US15467282

    申请日：2017-03-23

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Andrew Utada ,  Jeremy Agresti

    IPC: C12Q1/6834 ,  C08J3/12 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N33/58

    Abstract: A method of generating deformable gel beads that contain a single colloidal particle is described. Gel beads containing a particle are isolated from those that do not contain a particle, based on differences between the buoyant density of these gel bead populations. The gel beads containing a particle are subsequently co-encapsulated at a high efficiency into droplets that can comprise additional objects such as cells, other particles, or reagents. The gel coating on the rigid particle prevents clogging in narrow channels, such as those of a droplet generator.

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