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156 条记录 (耗时 0.034 秒)

    Nucleotide sequences which code for the sahH gene
    11.
    发明授权
    Nucleotide sequences which code for the sahH gene 有权
    编码sahH基因的核苷酸序列

    公开(公告)号：US06759224B2

    公开(公告)日：2004-07-06

    申请号：US09919854

    申请日：2001-08-02

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    IPC分类号： C12N914

    CPC分类号： C12N9/88 ,  A23K10/12 ,  A23K20/142 ,  A23K40/35 ,  A23K50/10 ,  C12P13/08 ,  C12P13/12

    摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.

    摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 多肽，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含至少15个连续的多核苷酸的多核苷酸 a），b）或c）的多核苷酸序列的核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sahH基因以增强形式存在，以及使用多核苷酸 含有根据本发明的序列作为杂交探针。

    Nucleotide sequences coding for the citE gene
    13.
    发明授权
    Nucleotide sequences coding for the citE gene 失效
    编码citE基因的核苷酸序列

    公开(公告)号：US07129066B2

    公开(公告)日：2006-10-31

    申请号：US10375355

    申请日：2003-02-28

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Brigitte Bathe ,  Walter Pfefferle

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Brigitte Bathe ,  Walter Pfefferle

    IPC分类号： C12P13/08 ,  C12P13/04 ,  C12N9/88 ,  C07H21/04

    CPC分类号： C12Y401/03006 ,  C12N9/88

    摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide that is at least 70% identical with a polynucleotide that codes for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) polynucleotide that codes for a polypeptide containing an amino acid sequence that is at least 70% identical with the amino acid sequence of SEQ ID No. 2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and to a process for the production of L-amino acids by fermentation using coryneform bacteria in which at least the citE gene is present in attenuated form, and to the use of polynucleotides containing the sequences of the invention as hybridization probes.

    摘要翻译： 本发明涉及含有多核苷酸序列的多核苷酸序列，该多核苷酸序列选自a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 对于含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少含有 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及通过发酵使用其中至少有citE基因以减毒形式存在的棒状细菌产生L-氨基酸的方法， 使用含有本发明序列的多核苷酸作为杂交探针。

    Nucleotide sequences which code for the dep34 gene
    15.
    发明授权
    Nucleotide sequences which code for the dep34 gene 失效
    编码dep34基因的核苷酸序列

    公开(公告)号：US07029904B2

    公开(公告)日：2006-04-18

    申请号：US09946763

    申请日：2001-09-06

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Walter Pfefferle ,  Thomas Hermann ,  Brigitte Bathe

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Walter Pfefferle ,  Thomas Hermann ,  Brigitte Bathe

    IPC分类号： C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C07K14/34 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the dep34 efflux protein, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及具有编码dep34流出蛋白的多核苷酸序列的谷氨酸棒杆菌分离的多核苷酸，以及具有其中dep34基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带至少 根据SEQ ID NO：1的dep34基因，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Process for the preparation of amino acids by using coryneform bacteria with attenuated 1-phosphofructokinase activity
    17.
    发明授权
    Process for the preparation of amino acids by using coryneform bacteria with attenuated 1-phosphofructokinase activity 失效
    通过使用具有减弱的1-磷酸果糖激酶活性的棒状细菌制备氨基酸的方法

    公开(公告)号：US06921651B2

    公开(公告)日：2005-07-26

    申请号：US10098626

    申请日：2002-03-18

    申请人： Mike Farwick ,  Brigitte Bathe ,  Jennifer Brehme ,  Klaus Huthmacher

    发明人： Mike Farwick ,  Brigitte Bathe ,  Jennifer Brehme ,  Klaus Huthmacher

    IPC分类号： C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12N15/55 ,  C12P13/04 ,  C12P13/08

    CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

    摘要： The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofructokinase are/is attenuated, enriching the desired L-amino acid in the medium or in the cells of the bacteria, and isolating the L-amino acid. Optionally bacteria are employed in which, in addition, further genes of the biosynthetic pathway of the desired L-amino acid are enhanced, or bacteria are employed in which the metabolic pathways that diminish the formation of the desired L-amino acid are at least partly switched off.

    摘要翻译： 本发明涉及一种制备L-氨基酸的方法。 该方法包括发酵产生所需L-氨基酸的棒状细菌，其中至少编码6-磷酸果糖激酶的基因和/或编码1-磷酸果糖激酶的基因被减毒，从而富集所需的L-氨基酸 培养基或细胞中的细胞，并分离L-氨基酸。 任选地，使用细菌，其中另外还需要增加所需L-氨基酸的生物合成途径的其它基因，或使用细菌，其中减少所需L-氨基酸形成的代谢途径至少部分地 关闭

    Nucleotide sequences which code for the menE gene
    18.
    发明授权
    Nucleotide sequences which code for the menE gene 失效
    编码menE基因的核苷酸序列

    公开(公告)号：US06946271B2

    公开(公告)日：2005-09-20

    申请号：US09834722

    申请日：2001-04-16

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Walter Pfefferle

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12N9/00 ,  C12P13/08

    CPC分类号： C12N9/93 ,  C12P13/08 ,  C12Y602/01026

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the menE gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含SEQ ID NO： 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少所述menE 基因以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Nucleotide sequences which code for the mikE17 gene
    20.
    发明授权
    Nucleotide sequences which code for the mikE17 gene 失效

    公开(公告)号：US07026158B2

    公开(公告)日：2006-04-11

    申请号：US09825293

    申请日：2001-04-04

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Walter Pfefferle

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Walter Pfefferle

    IPC分类号： C12N1/20

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the mikE17 gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.