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公开(公告)号：US20060177912A1
公开(公告)日：2006-08-10
申请号：US11274317
申请日：2005-11-16
摘要： The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the dep34 gene, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
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公开(公告)号：US07029904B2
公开(公告)日：2006-04-18
申请号：US09946763
申请日：2001-09-06
摘要： The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the dep34 efflux protein, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译： 本发明涉及具有编码dep34流出蛋白的多核苷酸序列的谷氨酸棒杆菌分离的多核苷酸，以及具有其中dep34基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带至少 根据SEQ ID NO：1的dep34基因，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
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公开(公告)号：US07038034B2
公开(公告)日：2006-05-02
申请号：US09948777
申请日：2001-09-10
摘要： The invention provides nucleotide sequences from Coryneform bacteria coding for the dep33 efflux protein and a process for the fermentative preparation of amino acids using bacteria in which the dep33 efflux protein is attenuated.
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4.发明授权Nucleotide sequences which code for the cysD, cysN, cysK, cysE and cysH genes 有权编码cysD，cysN，cysK，cysE和cysH基因的核苷酸序列公开(公告)号：US06822085B2
公开(公告)日：2004-11-23
申请号：US09962357
申请日：2001-09-26
申请人： Mike Farwick , Klaus Huthmacher , Natalie Schischka , Brigitte Bathe , Walter Pfefferle , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Mike Farwick , Klaus Huthmacher , Natalie Schischka , Brigitte Bathe , Walter Pfefferle , Michael Binder , Dieter Greissinger , Georg Thierbach
IPC分类号： C07H2104
CPC分类号： C12N9/0051 , A23K10/12 , A23K20/142 , A23K40/10 , C12N9/1029 , C12N9/1241 , C12N9/88 , C12P13/08 , C12P13/12
摘要： Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.
摘要翻译： 来自编码cysD，cysN，cysK，cysE和cysH基因的棒状细菌的核苷酸序列和使用其中所述基因的细菌发酵制备氨基酸的方法，L-氨基酸的发酵制备方法 使用其中至少cysD基因，cysN基因，cysK基因，cysE基因和/或cysH基因至少以增强形式存在的棒状细菌，以及含有根据本发明的序列的多核苷酸作为杂交探针 以及从发酵液中制备含有L-甲硫氨酸的动物饲料添加剂的方法。
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公开(公告)号：US06777206B2
公开(公告)日：2004-08-17
申请号：US09950071
申请日：2001-09-12
IPC分类号： C12P2106
摘要： The invention provides nucleotide sequences from Coryneform bacteria which code for the RodA protein and a process for the fermentative preparation of amino acids using bacteria in which the rodA gene is enhanced.
摘要翻译： 本发明提供了编码RodA蛋白质的棒状细菌的核苷酸序列和使用其中rodA基因增强的细菌发酵制备氨基酸的方法。
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公开(公告)号：US07129066B2
公开(公告)日：2006-10-31
申请号：US10375355
申请日：2003-02-28
申请人： Mike Farwick , Klaus Huthmacher , Achim Marx , Brigitte Bathe , Walter Pfefferle
发明人： Mike Farwick , Klaus Huthmacher , Achim Marx , Brigitte Bathe , Walter Pfefferle
CPC分类号： C12Y401/03006 , C12N9/88
摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide that is at least 70% identical with a polynucleotide that codes for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) polynucleotide that codes for a polypeptide containing an amino acid sequence that is at least 70% identical with the amino acid sequence of SEQ ID No. 2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and to a process for the production of L-amino acids by fermentation using coryneform bacteria in which at least the citE gene is present in attenuated form, and to the use of polynucleotides containing the sequences of the invention as hybridization probes.
摘要翻译： 本发明涉及含有多核苷酸序列的多核苷酸序列，该多核苷酸序列选自a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 对于含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少含有 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及通过发酵使用其中至少有citE基因以减毒形式存在的棒状细菌产生L-氨基酸的方法， 使用含有本发明序列的多核苷酸作为杂交探针。
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7.发明申请Nucleotide sequences which code for the cysD, cysN, cysK, cysE and cysH genes 审中-公开编码cysD，cysN，cysK，cysE和cysH基因的核苷酸序列公开(公告)号：US20050233424A1
公开(公告)日：2005-10-20
申请号：US10898345
申请日：2004-07-26
申请人： Mike Farwick , Klaus Huthmacher , Natalie Schischka , Brigitte Bathe , Walter Pfefferle , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Mike Farwick , Klaus Huthmacher , Natalie Schischka , Brigitte Bathe , Walter Pfefferle , Michael Binder , Dieter Greissinger , Georg Thierbach
IPC分类号： A23K1/00 , A23K1/16 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/88 , C12P13/08 , C12P13/12 , C07H21/04 , C12N15/74
CPC分类号： C12N9/0051 , A23K10/12 , A23K20/142 , A23K40/10 , C12N9/1029 , C12N9/1241 , C12N9/88 , C12P13/08 , C12P13/12
摘要： Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.
摘要翻译： 来自编码cysD，cysN，cysK，cysE和cysH基因的棒状细菌的核苷酸序列和使用其中所述基因的细菌发酵制备氨基酸的方法，L-氨基酸的发酵制备方法 使用其中至少cysD基因，cysN基因，cysK基因，cysE基因和/或cysH基因至少以增强形式存在的棒状细菌，以及含有根据本发明的序列的多核苷酸作为杂交探针 以及从发酵液中制备含有L-甲硫氨酸的动物饲料添加剂的方法。
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8.发明授权Process for the preparation of amino acids by using coryneform bacteria with attenuated 1-phosphofructokinase activity 失效通过使用具有减弱的1-磷酸果糖激酶活性的棒状细菌制备氨基酸的方法公开(公告)号：US06921651B2
公开(公告)日：2005-07-26
申请号：US10098626
申请日：2002-03-18
CPC分类号： C12N9/1205 , C12P13/04 , C12P13/08
摘要： The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofructokinase are/is attenuated, enriching the desired L-amino acid in the medium or in the cells of the bacteria, and isolating the L-amino acid. Optionally bacteria are employed in which, in addition, further genes of the biosynthetic pathway of the desired L-amino acid are enhanced, or bacteria are employed in which the metabolic pathways that diminish the formation of the desired L-amino acid are at least partly switched off.
摘要翻译： 本发明涉及一种制备L-氨基酸的方法。 该方法包括发酵产生所需L-氨基酸的棒状细菌，其中至少编码6-磷酸果糖激酶的基因和/或编码1-磷酸果糖激酶的基因被减毒，从而富集所需的L-氨基酸 培养基或细胞中的细胞，并分离L-氨基酸。 任选地，使用细菌，其中另外还需要增加所需L-氨基酸的生物合成途径的其它基因，或使用细菌，其中减少所需L-氨基酸形成的代谢途径至少部分地 关闭
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公开(公告)号：US20050074802A1
公开(公告)日：2005-04-07
申请号：US10936597
申请日：2004-09-09
申请人： Daniel Rey , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Brigitte Bathe , Klaus Huthmacher , Walter Pfefferle
发明人： Daniel Rey , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Brigitte Bathe , Klaus Huthmacher , Walter Pfefferle
IPC分类号： A23K10/12 , A23K20/142 , A23K40/10 , C07K14/34 , C12N1/21 , C12N15/31 , C12P13/12 , C12Q1/68 , C07H21/04 , C12N9/10 , C12N9/88 , C12N15/74
CPC分类号： C07K14/34 , A23K10/12 , A23K20/142 , A23K40/10 , C12P13/12
摘要： The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the metD gene and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of MetD transcription regulator activity, methods for preparation of L-amino acids, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of MetD transcription regulator activity. Further, the invention relates to animal food additives based on fermentation liquor and containing L-methionine, and to the preparation of such additive.
摘要翻译： 本发明涉及多核苷酸，其包含对应于metD基因的多核苷酸序列及其部分，其编码具有不同程度的MetD转录调节活性的多肽序列及其部分，用于制备L-氨基酸的方法，以及筛选和扩增编码多肽的多核苷酸的方法 包含不同程度的MetD转录调节活性的序列。 此外，本发明涉及基于发酵液并含有L-甲硫氨酸的动物性食品添加剂以及这种添加剂的制备。
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公开(公告)号：US06815196B2
公开(公告)日：2004-11-09
申请号：US09919831
申请日：2001-08-02
申请人： Brigitte Bathe , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Brigitte Bathe , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
IPC分类号： C12N120
摘要： The present invention relates to isolated polynucleotides from Corynebacterium glutamicum encoding proteins of the transcription activator (MetR) and O-succinylhomoserine sulfhydrylase (MetZ). The invention also relates to producing L-amino acids, particularly methionine, in coryneform cells having attenuated metR and metZ genes.
摘要翻译： 本发明涉及编码转录激活子（MetR）和O-琥珀酰高丝氨酸巯基酸酶（MetZ）的蛋白质的谷氨酸棒杆菌分离的多核苷酸。 本发明还涉及在具有减毒的metR和metZ基因的棒状细胞中产生L-氨基酸，特别是甲硫氨酸。
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