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28 条记录 (耗时 0.033 秒)

    Recombinant transferrins, transferrin half-molecules and mutants thereof
    13.
    发明申请
    Recombinant transferrins, transferrin half-molecules and mutants thereof 有权
    重组转运蛋白，转铁蛋白半分子及其突变体

    公开(公告)号：US20050054043A1

    公开(公告)日：2005-03-10

    申请号：US10887711

    申请日：2004-07-08

    申请人： Walter Funk ,  Ross MacGillivray ,  Anne Mason ,  Robert Woodworth

    发明人： Walter Funk ,  Ross MacGillivray ,  Anne Mason ,  Robert Woodworth

    IPC分类号： A61K38/00 ,  C07K14/79 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/705 ,  C07K14/78

    CPC分类号： C07K14/79 ,  A61K38/00

    摘要： Recombinant transferrin, non-glycosylated recombinant transferrin, transferrin half-molecules and mutant transferrins having altered metal-binding or other properties are described. The recombinant transferrin molecules are expressed in functional form by stable eukaryotic cell lines such as baby hamster kidney cells transformed with an expression vector encoding the recombinant molecule. The recombinant transferrins can be used in metal chelation therapy to bind and clear excess toxic metals in patients suffering from metal overloads or as tissue culture medium supplements or replacements.

    摘要翻译： 描述了具有改变的金属结合或其它性质的重组转铁蛋白，非糖基化重组转铁蛋白，转铁蛋白半分子和突变体转运蛋白。 重组转铁蛋白分子以稳定的真核细胞系如用编码重组分子的表达载体转化的幼仓鼠肾细胞以功能形式表达。 重组转移蛋白可用于金属螯合治疗，以结合和清除患有金属超负荷或组织培养基补充剂或替代物的患者中的过量有毒金属。

    Nucleic acid sequence of senescence asssociated gene
    17.
    发明授权
    Nucleic acid sequence of senescence asssociated gene 失效
    衰老相关基因的核酸序列

    公开(公告)号：US6025194A

    公开(公告)日：2000-02-15

    申请号：US974180

    申请日：1997-11-19

    申请人： Walter Funk

    发明人： Walter Funk

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85

    CPC分类号： C12Q1/6883 ,  C12Q1/6897 ,  C12Q2600/136 ,  C12Q2600/158

    摘要： Human gene GC6 is expressed more abundantly in senescent cells than young cells. Isolated, purified, and recombinant nucleic acids and proteins corresponding to the human GC6 gene and its mRNA and protein products, as well as peptides and antibodies corresponding to the GC6 protein can be used to identify senescent cells, distinguish between senescent and young cells, identify agents that alter senescent gene expression generally and GC6 expression specifically; such agents as well as GC6 gene and gene products and products corresponding thereto can be used to prevent and treat diseases and conditions relating to cell senescence.

    摘要翻译： 人类基因GC6在衰老细胞中的表达比年轻细胞更丰富。 对应于人GC6基因及其mRNA和蛋白质产物的分离，纯化和重组核酸和蛋白质以及与GC6蛋白相对应的肽和抗体可用于鉴定衰老细胞，区分衰老和年轻细胞，识别 通常改变衰老基因表达的药物和具体的GC6表达; 可以使用这样的试剂以及与其对应的GC6基因和基因产物和产物来预防和治疗与细胞衰老有关的疾病和病症。

    Method and Kit for enhanced differential display
    19.
    发明授权
    Method and Kit for enhanced differential display 失效
    用于增强差分显示的方法和工具包

    公开(公告)号：US5580726A

    公开(公告)日：1996-12-03

    申请号：US235180

    申请日：1994-04-29

    申请人： Bryant Villeponteau ,  Junli Feng ,  Walter Funk ,  Maarten H. K. Linskens

    发明人： Bryant Villeponteau ,  Junli Feng ,  Walter Funk ,  Maarten H. K. Linskens

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6881 ,  C12Q1/6809 ,  C12Q2600/158

    摘要： An improved method for detecting and isolating differentially expressed mRNAs which comprises using first oligonucleotide primers for reverse transcription of mRNAs and both the first oligonucleotide primers and second oligonucleotide primers for amplification of the resultant cDNAs. The improvement of this method comprises providing first and second oligonucleotide primers with a length of at least 21 oligonucleotides. The method further comprises using a two-step PCR amplification, wherein non-stringent conditions are used for the first 1 to 4 cycles, and stringent conditions are used for the next 16 to 22 cycles. This highly reproducible method will permit the preparation of comprehensive catalogs of gene expression for any given cell type.

    摘要翻译： 一种用于检测和分离差异表达的mRNA的改进方法，其包括使用第一寡核苷酸引物用于mRNA的逆转录，以及用于扩增所得cDNA的第一寡核苷酸引物和第二寡核苷酸引物。 该方法的改进包括提供长度为至少21个寡核苷酸的第一和第二寡核苷酸引物。 该方法还包括使用两步PCR扩增，其中非严格条件用于前1至4个循环，并且在接下来的16至22个循环中使用严格条件。 这种高度可重复的方法将允许为任何给定的细胞类型制备基因表达的综合目录。

    METHODS FOR TELOMERE LENGTH AND GENOMIC DNA QUALITY CONTROL AND ANALYSIS IN PLURIPOTENT STEM CELLS
    20.
    发明申请
    METHODS FOR TELOMERE LENGTH AND GENOMIC DNA QUALITY CONTROL AND ANALYSIS IN PLURIPOTENT STEM CELLS 审中-公开
    方法长期和基因组DNA质量控制和分析在大脑干细胞

    公开(公告)号：US20130011918A1

    公开(公告)日：2013-01-10

    申请号：US13579875

    申请日：2011-02-17

    申请人： Michael West ,  Karen B. Chapman ,  Walter Funk

    发明人： Michael West ,  Karen B. Chapman ,  Walter Funk

    IPC分类号： C12Q1/68 ,  G01N21/64 ,  C12N5/071 ,  C40B30/04

    CPC分类号： C12Q1/6881 ,  C12Q2600/158

    摘要： The generation of clinical-grade cell-based therapies from human embryonic stem cells or cells reprogrammed to pluripotency from somatic cells, requires stringent quality controls to insure that the cells have long enough telomeres and resulting cellular lifespan to be clinically useful, and normal gene expression and genomic integrity so as to insure cells with a desired and reproducible phenotype and to reduce the risk of the malignant transformation of cells. Assays useful in identifying human embryonic stem cell lines and pluripotent cells resulting from the transcriptional reprogramming of somatic cells that have embryonic telomere length are described as well as quality control assays for screening genomic integrity in cells expanded and banked for therapeutic use, as well as assays to identify cells capable of abnormal immortalization,

    摘要翻译： 从人类胚胎干细胞或从体细胞重编程为多能性的细胞产生临床级细胞基治疗需要严格的质量控制，以确保细胞具有足够长的端粒，并导致细胞寿命在临床上有用，并且正常的基因表达 和基因组完整性，以确保细胞具有所需和可重现的表型，并降低细胞恶性转化的风险。 描述了用于鉴定人胚胎干细胞系和由具有胚胎端粒长度的体细胞的转录重编程产生的多能细胞的测定以及用于筛选扩增和储存用于治疗用途的细胞中的基因组完整性的质量控制测定，以及测定 识别能够异常永生化的细胞，