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24 条记录 (耗时 0.026 秒)

    Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods
    11.
    发明授权
    Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods 有权
    含免疫原性HPV2V的VLP及相关组合物，构建体和治疗方法

    公开(公告)号：US09533057B2

    公开(公告)日：2017-01-03

    申请号：US13577484

    申请日：2011-02-08

    申请人： Bryce Chackerian ,  David S. Peabody

    发明人： Bryce Chackerian ,  David S. Peabody

    IPC分类号： C12N7/00 ,  A61K47/48 ,  A61K39/12 ,  A61K39/00

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K47/6901 ,  A61K2039/5258 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2795/18123

    摘要： The invention provides immunotherapeutic and prophylactic bacteriophage viral-like particle (VLPs) which are useful in the treatment and prevention of human papillomavirus (HPV) infections and related disorders, including cervical cancer and persistent infections associated with HPV. Related compositions (e.g. vaccines), nucleic acid constructs, and therapeutic methods are also provided. VLPs and related compositions of the invention induce high titer antibody responses against HPV L2 and protect against HPV challenge in vivo. VLPs, VLP-containing compositions, and therapeutic methods of the invention induce an immunogenic response against HPV infection, confer immunity against HPV infection, protect against HPV infection, and reduce the likelihood of infection by HPV infection.

    摘要翻译： 本发明提供免疫治疗和预防性噬菌体病毒样颗粒（VLP），其可用于治疗和预防人乳头状瘤病毒（HPV）感染和相关疾病，包括宫颈癌和与HPV相关的持续感染。 还提供了相关组合物（例如疫苗），核酸构建体和治疗方法。 本发明的VLP和相关组合物诱导针对HPV L2的高滴度抗体应答，并在体内保护免受HPV挑战。 VLP，含VLP的组合物和本发明的治疗方法诱导针对HPV感染的免疫原性应答，赋予HPV感染免疫，防止HPV感染，并降低HPV感染感染的可能性。

    Plasmids and methods for peptide display and affinity-selection on virus-like particles of RNA bacteriophages
    12.
    发明授权
    Plasmids and methods for peptide display and affinity-selection on virus-like particles of RNA bacteriophages 有权
    用于肽显示和亲和选择RNA质粒病毒样颗粒的质粒和方法

    公开(公告)号：US09365831B2

    公开(公告)日：2016-06-14

    申请号：US13520057

    申请日：2010-12-31

    申请人： David S. Peabody ,  Bryce Chackerian

    发明人： David S. Peabody ,  Bryce Chackerian

    IPC分类号： C40B40/08 ,  C12N7/00 ,  A61K39/07 ,  A61K39/12 ,  C12N15/10 ,  A61K39/00

    CPC分类号： A61K39/12 ,  A61K39/07 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/1037 ,  C12N2710/20022 ,  C12N2710/20034 ,  C12N2740/16034 ,  C12N2740/16134 ,  C12N2795/16021 ,  C12N2795/16022 ,  C12N2795/16023 ,  C12N2795/16042 ,  C12N2795/18023 ,  C40B40/08

    摘要： The present invention relates to a system and method for controlling peptide display valency on virus-like particles (VLPs), especially including MS2 VLPs. In this method, large amounts of wild-type and low quantities of single-chain dimer coat proteins may be produced from a single RNA. Valency is controlled in immunogen (vaccine) production by providing a system that allows the production of large amounts of wild-type and low quantities of single-chain dimer coating proteins from a single RNA, allowing facile adjustment of display valency levels on VLPs, especially MS2 VLPS over a wide range, from few than one—on average—to as many as ninety per particle. This facilitates the production of immunogens and vaccines, including VLPs exhibiting low valency. Nucleic acid constructs useful in the expression of virus-like particles are disclosed, comprised of a coat polypeptide of MS2 modified by insertion of a heterologous peptide, wherein the heterologous peptide is displayed on the virus-like particle and encapsidates MS2 niRNA. Nucleic acid constructs are also disclosed which are useful in the expression of virus-like particles comprised of a coat polypeptide of PP7 modified by insertion of a heterologous peptide, wherein the heterologous peptide is displayed on the virus-like particle and encapsidates PP7 mRNA.

    摘要翻译： 本发明涉及一种用于控制病毒样颗粒（VLPs），特别是包括MS2 VLP的肽显示效价的系统和方法。 在该方法中，可以从单个RNA产生大量野生型和低量的单链二聚体外壳蛋白。 通过提供允许从单个RNA产生大量野生型和少量单链二聚体包被蛋白质的系统，允许轻度调整VLP上的显示剂价位水平，特别是 MS2 VLPS在广泛的范围内，从一个平均至少一个到每个粒子多达九十个。 这有助于免疫原和疫苗的生产，包括显示低效价的VLP。 公开了可用于病毒样颗粒表达的核酸构建物，其由通过插入异源肽修饰的MS2的外壳多肽组成，其中异源肽显示在病毒样颗粒上并包裹MS2nRNA。 还公开了核酸构建体，其可用于由通过插入异源肽修饰的PP7的外壳多肽组成的病毒样颗粒的表达，其中异源肽显示在病毒样颗粒上并包裹PP7 mRNA。

    PLASMIDS AND METHODS FOR PEPTIDE DISPLAY AND AFFINITY-SELECTION ON VIRUS-LIKE PARTICLES OF RNA BACTERIOPHAGES
    13.
    发明申请
    PLASMIDS AND METHODS FOR PEPTIDE DISPLAY AND AFFINITY-SELECTION ON VIRUS-LIKE PARTICLES OF RNA BACTERIOPHAGES 审中-公开
    肽类显示的方法和方法及RNA病毒类病毒颗粒的选择

    公开(公告)号：US20140106982A1

    公开(公告)日：2014-04-17

    申请号：US14104844

    申请日：2013-12-12

    申请人： David S. Peabody ,  Bryce Chackerian

    发明人： David S. Peabody ,  Bryce Chackerian

    IPC分类号： G01N33/569

    CPC分类号： G01N33/56983 ,  C12N15/1037 ,  C12N2795/14021 ,  C12N2795/14023

    摘要： The present invention relates to a system and method for controlling peptide display valency on virus-like particles (VLPs), especially including MS2 or PP7 VLPs. In this method, large amounts of wild-type and low quantities of single-chain dimer coat proteins may be produced from a single RNA. Valency is controlled in immunogen (vaccine) production by providing a system that allows the production of large amounts of wild-type and low quantities of single-chain dimer coating proteins from a single RNA, allowing facile adjustment of display valency levels on bacteriophage VLPs, especially MS2 or PP7 VLPs over a wide range, from few than one—on average—to as many as ninety per particle. This facilitates the production of immunogens and vaccines, including VLPs exhibiting low valency. Nucleic acid constructs useful in the expression of virus-like particles are disclosed, comprised of a coat polypeptide of bacteriophage such as MS2 or PP7 modified by insertion of a heterologous peptide, optionally comprising a carbohydrate mimotope, wherein the heterologous peptide is displayed on the virus-like particle and encapsidates bacteriphage mRNA.

    摘要翻译： 本发明涉及用于控制病毒样颗粒（VLPs），特别是包括MS2或PP7VLP的肽显示剂价态的系统和方法。 在该方法中，可以从单个RNA产生大量野生型和低量的单链二聚体外壳蛋白。 通过提供允许从单个RNA产生大量野生型和少量单链二聚体包被蛋白的系统，允许容易地调节噬菌体VLP上的显示价态水平，从而在免疫原（疫苗）生产中控制价值， 特别是在广泛范围内的MS2或PP7 VLP，从一个平均至少一个到每个颗粒多达九十个。 这有助于免疫原和疫苗的生产，包括显示低效价的VLP。 公开了可用于病毒样颗粒表达的核酸构建体，其包括噬菌体的外壳多肽，例如通过插入异源肽修饰的MS2或PP7，任选地包含碳水化合物模拟表位，其中异源肽显示在病毒上 样颗粒并包裹细菌噬菌体mRNA。