METHODS FOR DNA AND RNA EXTRACTION FROM FIXED PARAFFIN-EMBEDDED TISSUE SAMPLES

    公开(公告)号:US20190225959A1

    公开(公告)日:2019-07-25

    申请号:US16255659

    申请日:2019-01-23

    Applicant: Cepheid

    Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.

    Honeycomb tube
    15.
    发明授权

    公开(公告)号:US12281358B2

    公开(公告)日:2025-04-22

    申请号:US18350916

    申请日:2023-08-07

    Applicant: Cepheid

    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

    HONEYCOMB TUBE
    17.
    发明申请

    公开(公告)号:US20210164044A1

    公开(公告)日:2021-06-03

    申请号:US16992392

    申请日:2020-08-13

    Applicant: Cepheid

    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

    HONEYCOMB TUBE
    18.
    发明申请

    公开(公告)号:US20210147933A1

    公开(公告)日:2021-05-20

    申请号:US17103688

    申请日:2020-11-24

    Applicant: Cepheid

    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.

    HONEYCOMB TUBE
    20.
    发明申请
    HONEYCOMB TUBE 审中-公开
    蜂窝管

    公开(公告)号:US20150275292A1

    公开(公告)日:2015-10-01

    申请号:US14431259

    申请日:2013-09-26

    Applicant: CEPHEID

    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

    Abstract translation: 一种蜂窝管,其具有限定在第一平面和第二平面之间的流体路径的平面框架。 流体界面位于平面框架的一端。 流体界面具有流体入口和流体出口。 流体路径还包括具有多个孔的具有良好基底的井室。 阱室布置在第一或第二表面和阱基板之间的平面框架中。

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