公开(公告)号：US20220226809A1

公开(公告)日：2022-07-21

申请号：US17586645

申请日：2022-01-27

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Andrew Kohlway ,  Reuel Van Atta ,  Russell Higuchi ,  Alexander A. Gall ,  Kriszten Kocmond

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6827 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

Abstract: Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and/or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

公开(公告)号：US10233440B2

公开(公告)日：2019-03-19

申请号：US14431243

申请日：2013-09-26

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Reuel Van Atta ,  Kenneth E. Ho

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6886 ,  G06F19/20 ,  G01N1/06

Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.

公开(公告)号：US12203135B2

公开(公告)日：2025-01-21

申请号：US18481156

申请日：2023-10-04

Applicant: Cepheid

Inventor： Yuh-Min Chiang ,  Douglas Dority ,  Dustin Dickens ,  Jennifer Glass ,  Reuel Van Atta

IPC: C12Q1/6876 ,  B01L3/00 ,  B01L7/00

Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.

公开(公告)号：US20240068030A1

公开(公告)日：2024-02-29

申请号：US18481156

申请日：2023-10-04

Applicant: Cepheid

Inventor： Yuh-Min Chiang ,  Douglas Dority ,  Dustin Dickens ,  Jennifer Glass ,  Reuel Van Atta

IPC: C12Q1/6876 ,  B01L3/00

CPC classification number: C12Q1/6876 ,  B01L3/502 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52

Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.

公开(公告)号：US11739383B2

公开(公告)日：2023-08-29

申请号：US16992392

申请日：2020-08-13

Applicant: Cepheid

Inventor： Yuh-Min Chiang ,  Doug Dority ,  Dustin Dickens ,  Jennifer Glass ,  Reuel Van Atta

IPC: B01L3/00 ,  C12Q1/6876 ,  B01L7/00

CPC classification number: C12Q1/6876 ,  B01L3/502 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2400/0406 ,  B01L2400/0487

Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

公开(公告)号：US11260387B2

公开(公告)日：2022-03-01

申请号：US15839731

申请日：2017-12-12

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Andrew Kohlway ,  Reuel Van Atta ,  Russell Higuchi ,  Alexander A. Gall ,  Kriszten Kocmond

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6827 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

Abstract: Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and/or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

公开(公告)号：US10767226B2

公开(公告)日：2020-09-08

申请号：US16230458

申请日：2018-12-21

Applicant: Cepheid

Inventor： Yuh-Min Chiang ,  Douglas B Dority ,  Dustin Dickens ,  Jennifer Glass ,  Reuel Van Atta

IPC: C12Q1/6876 ,  B01L3/00 ,  B01L7/00

Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

公开(公告)号：US20190211396A1

公开(公告)日：2019-07-11

申请号：US16230458

申请日：2018-12-21

Applicant: Cepheid

Inventor： YUH-MIN CHIANG ,  Douglas B. Dority ,  Dustin Dickens ,  Jennifer Glass ,  Reuel Van Atta

IPC: C12Q1/6876 ,  B01L3/00

Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

公开(公告)号：US10190165B2

公开(公告)日：2019-01-29

申请号：US14431259

申请日：2013-09-26

Applicant: CEPHEID

Inventor： Yuh-Min Chiang ,  Doug Dority ,  Dustin Dickens ,  Jennifer Glass ,  Reuel Van Atta

IPC: C12Q1/68 ,  C12Q1/6876 ,  B01L3/00 ,  B01L7/00

Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

公开(公告)号：US20240141415A1

公开(公告)日：2024-05-02

申请号：US18095982

申请日：2023-01-11

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Andrew Kohlway ,  Reuel Van Atta ,  Russell Higuchi ,  Alexander A. Gall ,  Kriszten Kocmond

IPC: C12Q1/6827 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6886

CPC classification number: C12Q1/6827 ,  B01L7/52 ,  C12N15/1006 ,  C12N15/1017 ,  C12Q1/6806 ,  C12Q1/6886 ,  B01L2300/0864 ,  B01L2300/087 ,  C12Q2600/154

Abstract: In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.