公开(公告)号：US20230357780A1

公开(公告)日：2023-11-09

申请号：US18317915

申请日：2023-05-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash FARHADI ,  Gabrielle H HO ,  Daniel P SAWYER ,  Mikhail SHAPIRO ,  Robert C. HURT ,  Mengtong DUAN

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US20230277695A1

公开(公告)日：2023-09-07

申请号：US18046881

申请日：2022-10-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Anupama LAKSHMANAN ,  Arash FARHADI ,  Suchita P. NETY ,  Raymond W. BOURDEAU ,  Mikhail SHAPIRO

IPC: A61K49/22 ,  A61B8/08 ,  C07K14/195 ,  A61K49/00

CPC classification number: A61K49/223 ,  A61B8/481 ,  A61K49/0002 ,  A61K49/0045 ,  A61K49/0093 ,  A61K49/221 ,  C07K14/195 ,  A61B8/5238 ,  C07K2319/00

Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

公开(公告)号：US20220346811A1

公开(公告)日：2022-11-03

申请号：US17813177

申请日：2022-07-18

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Saransh SHARMA ,  Abhinav AGARWAL ,  Mikhail SHAPIRO ,  Azita EMAMI

IPC: A61B17/17

Abstract: A three dimensional magnetic sensor attached to a surgical nail is located based on an applied monotonic magnetic field gradient. Another three dimensional magnetic sensor locates a surgical drill. A display generates a real time image of the relative alignment of the surgical drill and of the surgical nail, allowing a surgeon to repair bone fractures.

公开(公告)号：US20200306564A1

公开(公告)日：2020-10-01

申请号：US16833637

申请日：2020-03-29

Applicant: California Institute of Technology

Inventor： Avinoam D. BAR-ZION ,  Atousa NOURMAHNAD ,  David MARESCA ,  Mikhail SHAPIRO

IPC: A61N7/00

Abstract: The system and process of therapeutic and effective cavitation by using ultrasound to collapse gas vesicles as well as cavitate the bubbles produced from the collapsed gas vesicles. Therapeutic effect includes, but is not limited to lysing cells by cavitation. The cells expressing the gas vesicles can optionally be used as delivery cells to preform tasks such as transporting the gas vesicles into deep tissue areas, releasing compounds at the cavitation site, and more. The gas vesicles can optionally be modified to facilitate getting the bubbles near the cavitation targets by functionalizing the gas vesicles.