公开(公告)号：US11535882B2

公开(公告)日：2022-12-27

申请号：US15084307

申请日：2016-03-29

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Stephen P. A. Fodor

IPC: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869

Abstract: The present disclosure provide compositions, methods and kits for generating a set of combinatorial barcodes, and uses thereof for barcoding samples such as single cells or genomic DNA fragments. Some embodiments disclosed herein provide compositions comprising a set of component barcodes for producing a set of combinatorial barcodes. The set of component barcodes can comprise, for example, n×m unique component barcodes, wherein n and m are integers, each of the component barcodes comprises: one of n unique barcode subunit sequences; and one or two linker sequences or the complements thereof, wherein the component barcodes are configured to connect to each other through the one or two linker sequences or the complements thereof to produce a set of combinatorial barcodes.

公开(公告)号：US10059991B2

公开(公告)日：2018-08-28

申请号：US15217896

申请日：2016-07-22

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Glenn K. Fu

IPC: C12N15/10 ,  G06F19/20 ,  G06F19/22 ,  C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q1/6851

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B30/00 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US09708659B2

公开(公告)日：2017-07-18

申请号：US14540029

申请日：2014-11-12

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Glenn K. Fu

IPC: C40B60/08 ,  G01N33/48 ,  C40B20/04 ,  C40B30/04 ,  C12Q1/68 ,  C12N15/10 ,  G06F19/20 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US09637799B2

公开(公告)日：2017-05-02

申请号：US14872377

申请日：2015-10-01

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US11618929B2

公开(公告)日：2023-04-04

申请号：US16012584

申请日：2018-06-19

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US10954570B2

公开(公告)日：2021-03-23

申请号：US15459977

申请日：2017-03-15

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn K. Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US09567646B2

公开(公告)日：2017-02-14

申请号：US14800526

申请日：2015-07-15

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US10941396B2

公开(公告)日：2021-03-09

申请号：US14381488

申请日：2013-02-27

Applicant: Cellular Research, Inc.

Inventor： Glenn K. Fu ,  Stephen P. A. Fodor ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: Methods, kits and systems are disclosed for analyzing one or more molecules in a sample. Analyzing the one or more molecules may comprise quantitation of the one or more molecules. Individual molecules may quantitated by PCR, arrays, beads, emulsions, droplets, or sequencing. Quantitation of individual molecules may further comprise stochastic labeling of the one or more molecules with a plurality of oligonucleotide tags to produce one or more stochastically labeled molecules. The methods may further comprise amplifying, sequencing, detecting, and/or quantifying the stochastically labeled molecules. The molecules may be DNA, RNA and/or proteins.

公开(公告)号：US10151003B2

公开(公告)日：2018-12-11

申请号：US16038979

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US10047394B2

公开(公告)日：2018-08-14

申请号：US15224460

申请日：2016-07-29

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Glenn K. Fu

IPC: C12N15/10 ,  G06F19/20 ,  G06F19/22 ,  C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q1/6851

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B30/00 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.