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公开(公告)号：US10047368B2
公开(公告)日：2018-08-14
申请号：US14514837
申请日：2014-10-15
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Navin Elango , Karthik N. Muthuraman , Jeffrey Beringer , Sara Bennett , Huixia Wu
IPC: C12N15/82 , C12N15/113
Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5′-UTRs, introns, and/or 3′-UTRs, isolated from Zea mays.
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12.发明授权公开(公告)号：US10017775B2
公开(公告)日：2018-07-10
申请号：US15471545
申请日：2017-03-28
Applicant: Dow AgroSciences LLC , Sangamo Therapeutics, Inc.
Inventor： Russell DeKelver , Manju Gupta , Jeffrey C. Miller , Stephen Novak , Joseph F. Petolino
IPC: C12N15/82
CPC classification number: C12N15/8218 , C07K14/415 , C12N9/22 , C12N15/8216 , C12N15/8247 , C12Y203/01041 , C12Y301/00
Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.
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公开(公告)号：US20170334957A1
公开(公告)日：2017-11-23
申请号：US15485338
申请日：2017-04-12
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sandeep Kumar , Wei Chen
IPC: C07K14/415 , C12N15/82 , C12N15/00
CPC classification number: C12N15/8222 , C12N15/00 , C12N15/8216 , C12N15/8225 , C12N15/8241 , C12N15/8286 , Y02A40/162
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
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14.发明授权公开(公告)号：US09752155B2
公开(公告)日：2017-09-05
申请号：US14472977
申请日：2014-08-29
Applicant: Dow AgroSciences, LLC
Inventor： Sandeep Kumar , Manju Gupta , Diaa Alabed
IPC: C12N15/82 , C07K14/415
CPC classification number: C12N15/8216 , C07K14/415
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Brachypodium distachyon ubiquitin genes.
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公开(公告)号：US20170081676A1
公开(公告)日：2017-03-23
申请号：US15266370
申请日：2016-09-15
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sara Bennett , Andrew F. Worden
IPC: C12N15/82
CPC classification number: C12N15/8216 , C12N15/8222 , C12N15/8274 , C12N15/8286 , Y02A40/162
Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3′UTR from Arabidopsis thaliana Ubiquitin-10 gene. Some embodiments relate to a 3′ UTR from a Arabidopsis thaliana Ubiquitin-10 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.
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Patent Agency Ranking
16.发明申请公开(公告)号：US20170029833A1
公开(公告)日：2017-02-02
申请号：US15212000
申请日：2016-07-15
Applicant: Dow AgroSciences LLC
Inventor： Sandeep Kumar , Diaa Alabed , Terry R. Wright , Manju Gupta
IPC: C12N15/82 , C07K14/005
CPC classification number: C12N15/8216 , C07K14/005 , C12N15/8222 , C12N15/8286 , C12N2770/00022 , Y02A40/162
Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 aerie or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.
Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1通气或其功能等同物的最小核心启动子元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。
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17.发明授权公开(公告)号：US09453235B2
公开(公告)日：2016-09-27
申请号：US13674712
申请日：2012-11-12
Applicant: Dow AgroSciences LLC
Inventor： Sandeep Kumar , Diaa Alabed , Sara Bennett , Manju Gupta , Susan Jayne , Terry R. Wright
IPC: C12N15/82
CPC classification number: C12N15/8216 , C12N15/8286 , Y02A40/162
Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.
Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。
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公开(公告)号：US20150167076A1
公开(公告)日：2015-06-18
申请号：US14577063
申请日：2014-12-19
Applicant: E. I DuPont de Nemours and Company , Pioneer Hi-Bred International, Inc. , Dow Agrosciences LLC
Inventor： James Wayne Bing , Robert F. Cressman, JR. , Manju Gupta , Salim M. Hakimi , David Hondred , Todd L. Krone , Mary E. Hartnett Locke , Abigail K. Luckring , Sandra E. Meyer , Daniel Moellenbeck , Kenneth Edwin Narva , Paul D. Olson , Craig D. Sanders , Jimei Wang , Jian Zhang , Gan-Yuan Zhong
IPC: C12Q1/68
CPC classification number: C12Q1/6876 , C07K14/415 , C12N15/8277 , C12N15/8286 , C12Q1/6895 , C12Q2600/13 , Y02A40/162
Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
Abstract translation: 本发明提供了涉及转基因抗虫玉米植物的DNA组合物。 还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DAS-59122-7事件的存在的测定。 提供了用于进行测定的试剂盒和条件。
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公开(公告)号：US20150072896A1
公开(公告)日：2015-03-12
申请号：US14547589
申请日：2014-11-19
Applicant: Pioneer Hi-Bred International, Inc. , Dow Agrosciences LLC , E. I. Du Pont De Nemours and Company
Inventor： Eric Barbour , James Wayne Bing , Guy A. Cardineau , Robert F. Cressman, JR. , Manju Gupta , Mary E. Hartnett Locke , David Hondred , Joseph W. Keaschall , Michael G. Koziel , Terry EuClaire Meyer , Daniel Moellenbeck , Kenneth Edwin Narva , Wilas Nirunsuksiri , Stephen W. Ritchie , Marjorie L. Rudert , Craig D. Sanders , Aihua Shao , Steven Jeffrey Stelman , David S. Stucker , Laura Ann Tagliani , William M. Van Zante
IPC: C12Q1/68
CPC classification number: C12Q1/6895 , C12N15/8277 , C12N15/8286 , C12Q1/686 , C12Q2600/13 , C12Q2600/16 , Y02A40/162 , Y10S435/975
Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize TC1507 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
Abstract translation: 本发明提供了与转基因抗虫玉米植物相关的DNA组合物。 还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米TC1507事件的存在的测定法。 提供了用于进行测定的试剂盒和条件。
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公开(公告)号：US10526610B2
公开(公告)日：2020-01-07
申请号：US15882609
申请日：2018-01-29
Applicant: Dow AgroSciences LLC , Sangamo Therapeutics, Inc.
Inventor： Noel Cogan , John Forster , Matthew Hayden , Tim Sawbridge , German Spangenberg , Steven R. Webb , Manju Gupta , W. Michael Ainley , Matthew J. Henry , Jeffrey C. Miller , Dmitry Y. Guschin
Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
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