公开(公告)号：US20200377899A1

公开(公告)日：2020-12-03

申请号：US16994734

申请日：2020-08-17

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Ryan M. Lee ,  Stephen Novak ,  Andrew F. Worden

IPC: C12N15/82

Abstract: The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.

公开(公告)号：US20180163218A1

公开(公告)日：2018-06-14

申请号：US15833168

申请日：2017-12-06

Applicant: Dow AgroSciences LLC

Inventor： David H. Corbin ,  Wei Chen ,  Stephen Novak ,  Ryan M. Lee ,  Sandeep Kumar ,  Andrew Asberry ,  Andrew F. Worden

IPC: C12N15/82 ,  C12N9/22

Abstract: Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

公开(公告)号：US20180100162A1

公开(公告)日：2018-04-12

申请号：US15705312

申请日：2017-09-15

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Marcelo Ariel German ,  Pohao Wang ,  Todd P. Glancy ,  Shreedharan Sriram ,  Carla N. Yerkes ,  Andrew J. Bowling ,  Heather Pence ,  Andrew E. Robinson

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  C12N15/8223 ,  C12N15/8234 ,  C12N15/8274

Abstract: This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

公开(公告)号：US20180087064A1

公开(公告)日：2018-03-29

申请号：US15835620

申请日：2017-12-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82 ,  C07K14/825

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US09914933B2

公开(公告)日：2018-03-13

申请号：US15097345

申请日：2016-04-13

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82

CPC classification number: C12N15/8227

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Brachypodium distachyon metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Brachypodium distachyon metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20170334957A1

公开(公告)日：2017-11-23

申请号：US15485338

申请日：2017-04-12

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C07K14/415 ,  C12N15/82 ,  C12N15/00

CPC classification number: C12N15/8222 ,  C12N15/00 ,  C12N15/8216 ,  C12N15/8225 ,  C12N15/8241 ,  C12N15/8286 ,  Y02A40/162

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US09752155B2

公开(公告)日：2017-09-05

申请号：US14472977

申请日：2014-08-29

Applicant: Dow AgroSciences, LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C07K14/415

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Brachypodium distachyon ubiquitin genes.

公开(公告)号：US20170029833A1

公开(公告)日：2017-02-02

申请号：US15212000

申请日：2016-07-15

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry R. Wright ,  Manju Gupta

IPC: C12N15/82 ,  C07K14/005

CPC classification number: C12N15/8216 ,  C07K14/005 ,  C12N15/8222 ,  C12N15/8286 ,  C12N2770/00022 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 aerie or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1通气或其功能等同物的最小核心启动子元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US09453235B2

公开(公告)日：2016-09-27

申请号：US13674712

申请日：2012-11-12

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US20160130594A1

公开(公告)日：2016-05-12

申请号：US14938632

申请日：2015-11-11

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Jeffrey R. Berringer ,  Wei Chen ,  Andrew M. Asberry

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8209 ,  C12N15/8286 ,  Y02A40/162

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Zea mays Ubiquitin-1 gene promoter, and the full-length nucleotide sequence elements from a Rice Ubiquitin-3 promoter. Some embodiments relate to a synthetic bi-directional promoter that may function in plants to promote transcription of two operably linked nucleotide sequences.

Abstract translation: 本公开涉及用于促进植物或植物细胞中核苷酸序列的转录的组合物和方法，其使用来自玉米泛素-1基因启动子的最小核心启动子元件和来自水稻泛素-3的全长核苷酸序列元件 启动子。 一些实施方案涉及可在植物中起作用以促进两个可操作地连接的核苷酸序列的转录的合成双向启动子。