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14 条记录 (耗时 0.026 秒)

    Method for reducing loss of enzyme activity during storage
    11.
    发明授权
    Method for reducing loss of enzyme activity during storage 有权
    减少贮藏期间酶活性降低的方法

    公开(公告)号：US07776572B2

    公开(公告)日：2010-08-17

    申请号：US11707851

    申请日：2007-02-16

    申请人： Dana Craig Bookbinder ,  Edward John Fewkes, Jr. ,  James Arthur Griffin ,  Frances M. Smith ,  David L. Tennent

    发明人： Dana Craig Bookbinder ,  Edward John Fewkes, Jr. ,  James Arthur Griffin ,  Frances M. Smith ,  David L. Tennent

    IPC分类号： C12N9/00 ,  C12N11/00 ,  C12N5/00 ,  C12N1/00 ,  C12M1/00 ,  G01N33/53 ,  C07K17/00 ,  C07H21/00 ,  A61K38/00 ,  A01N1/02 ,  C12Q1/00

    CPC分类号： C07H21/00 ,  B01L3/50 ,  B01L3/5082 ,  B01L2200/12 ,  B01L2300/16 ,  B05D7/02 ,  C07B2200/11 ,  C08J7/047 ,  C08J7/065 ,  C08J2471/00 ,  C09D5/00 ,  G01N33/54393

    摘要： Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.

    摘要翻译： 蛋白质结合水平小于约50-80ng / cm 2的疏水性聚合物表面通过以下方式实现：（1）将由溶剂和HLB数小于5的非离子表面活性剂组成的涂布溶液施加到表面 ; 和（2）干燥表面以除去溶剂，从而使表面活性剂与疏水性聚合物直接接触。 已经发现低HLB值和干燥步骤的组合产生低结合表面，其可以经受水和/或蛋白质溶液的多次洗涤。或者，低结合表面可以通过将非离子表面活性剂施加到 接触熔融聚合物并将聚合物形成所需形状的模具表面，例如多孔板，移液管尖端等。 此外，低粘合表面可以通过将HLB数小于或等于10的非可溶性非离子表面活性剂在模制制品之前加入到聚合物共混物中来制备。

    Method for reducing binding of organic material to hydrophobic surfaces
    12.
    发明授权
    Method for reducing binding of organic material to hydrophobic surfaces 有权
    减少有机材料与疏水性表面结合的方法

    公开(公告)号：US07312057B2

    公开(公告)日：2007-12-25

    申请号：US10378974

    申请日：2003-03-03

    申请人： Dana Craig Bookbinder ,  Edward John Fewkes, Jr. ,  James Arthur Griffin ,  Frances M. Smith ,  David L. Tennent

    发明人： Dana Craig Bookbinder ,  Edward John Fewkes, Jr. ,  James Arthur Griffin ,  Frances M. Smith ,  David L. Tennent

    IPC分类号： C12N9/00 ,  G01N33/53 ,  C07K17/00 ,  C07H21/00

    CPC分类号： C07H21/00 ,  B01L3/50 ,  B01L3/5082 ,  B01L2200/12 ,  B01L2300/16 ,  B05D7/02 ,  C07B2200/11 ,  C08J7/047 ,  C08J7/065 ,  C08J2471/00 ,  C09D5/00 ,  G01N33/54393

    摘要： Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.

    摘要翻译： 蛋白质结合水平小于约50-80ng / cm 2的疏水性聚合物表面通过以下方式实现：（1）施加由溶剂和具有HLB的非离子表面活性剂组成的涂布溶液 数量小于5的表面; 和（2）干燥表面以除去溶剂，从而使表面活性剂与疏水性聚合物直接接触。 已经发现低HLB值和干燥步骤的组合产生低结合表面，其可以经受水和/或蛋白质溶液的多次洗涤。或者，低结合表面可以通过将非离子表面活性剂施加到 接触熔融聚合物并将聚合物形成所需形状的模具表面，例如多孔板，移液管尖端等。 此外，低粘合表面可以通过将HLB数小于或等于10的非可溶性非离子表面活性剂在模制制品之前加入到聚合物共混物中来制备。