公开(公告)号：US20230193354A1

公开(公告)日：2023-06-22

申请号：US17992564

申请日：2022-11-22

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Junhua ZHAO ,  Molly HE ,  Samantha SNOW ,  William LIGHT ,  Matthew KELLINGER ,  Michael PREVITE ,  Michael KIM ,  Hua YU ,  Yu-Hsien HWANG-FU ,  Marco TJIOE ,  Andrew BODDICKER

IPC: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/64 ,  C12Q1/6853

CPC classification number: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6458 ,  C12Q1/6853 ,  C12Q2600/158

Abstract: The disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

公开(公告)号：US20210387184A1

公开(公告)日：2021-12-16

申请号：US17218027

申请日：2021-03-30

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE ,  Sinan ARSLAN ,  Molly HE ,  Huizhen MAH ,  Lei SUN

IPC: B01L3/00 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6806 ,  G01N21/64

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20210123098A1

公开(公告)日：2021-04-29

申请号：US17144945

申请日：2021-01-08

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Michael PREVITE ,  Molly HE ,  Junhua ZHAO ,  Hui Zhen MAH ,  Chunhong ZHOU ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Lorenzo BERTI ,  Steve CHEN

IPC: C12Q1/6869 ,  C12Q1/6841

Abstract: Provided are methods and systems for analyzing nucleic acids in a biological sample in a manner that retains the spatial and/or cellular origin of the nucleic acids within the biological sample. Compositions and kits are also provided that enable the methods and systems of the instant disclosure.

公开(公告)号：US20240382956A1

公开(公告)日：2024-11-21

申请号：US18680203

申请日：2024-05-31

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE

IPC: B01L3/00

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20240287482A1

公开(公告)日：2024-08-29

申请号：US18650856

申请日：2024-04-30

Applicant: Element Biosciences, Inc.

Inventor： Jendrik HENTSCHEL ,  Tyler LOPEZ ,  Michael KLEIN ,  Virginia SAADE ,  Matthew KELLINGER ,  Mark AMBROSO

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US20230323450A1

公开(公告)日：2023-10-12

申请号：US18181476

申请日：2023-03-09

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly Min HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: C12Q1/6874 ,  G01N33/58 ,  G01N33/53

CPC classification number: C12Q1/6874 ,  G01N33/582 ,  G01N33/5308

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20230265400A1

公开(公告)日：2023-08-24

申请号：US18160947

申请日：2023-01-27

Applicant: Element Biosciences, Inc.

Inventor： Jendrik HENTSHCEL ,  Tyler LOPEZ ,  Michael KLEIN ,  Virginia SAADE ,  Matthew KELLINGER ,  Mark AMBROSO

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US20230235392A1

公开(公告)日：2023-07-27

申请号：US17999023

申请日：2021-05-19

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Junhua ZHAO ,  Molly HE ,  William LIGHT ,  Matthew KELLINGER ,  Michael PREVITE

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6876 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2600/16

Abstract: Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing. Nucleic acid sequence methods, systems and kits are also provided for sequencing circular nucleic acid molecules.

公开(公告)号：US20230167434A1

公开(公告)日：2023-06-01

申请号：US18153268

申请日：2023-01-11

Applicant: Element Biosciences, Inc.

Inventor： Matthew KELLINGER ,  Sinan ARSLAN ,  Michael PREVITE ,  Junhua ZHAO

IPC: C12N15/10 ,  C12N9/12 ,  C12Q1/6869

CPC classification number: C12N15/1068 ,  C12N9/12 ,  C12N9/1241 ,  C12Q1/6869

Abstract: Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing.

公开(公告)号：US20220186310A1

公开(公告)日：2022-06-16

申请号：US17511046

申请日：2021-10-26

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly Min HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: C12Q1/6874 ,  G01N33/53 ,  G01N33/58

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.