公开(公告)号：US07648835B2

公开(公告)日：2010-01-19

申请号：US12199613

申请日：2008-08-27

申请人： Wayne L. Breidford ,  Christy A. Lancaster ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Jeffrey F. Tonn ,  Bernhard H. Weigl

发明人： Wayne L. Breidford ,  Christy A. Lancaster ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Jeffrey F. Tonn ,  Bernhard H. Weigl

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： B01L3/5027 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2300/1877

摘要： An integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.

摘要翻译： 微流控卡上集成的热交换系统。 根据本发明的一个方面，便携式微流控卡具有板上的加热，冷却和热循环系统，使得卡可以可移动地使用。 微流控卡包括一个或多个含有放热或吸热材料的储存器。 一旦储存器材料的化学过程被激活，储存器就向微流控卡的特定位置提供热量或冷却。 可以在单个卡上包括多个储存器以提供变化的温度。 测定化学品可以移动到各种储存器以产生可用于许多生物反应的热循环，例如聚合酶链式反应（PCR）或rtPCR。 根据本发明的另一方面，集成热交换器是包含独立加热或冷却的流体的相邻微流体回路，或者是放热或吸热材料，使得相邻回路中的流体将温度变化赋予 独立电路中的分析流体。 根据本发明的另一方面，热电冷却器（TEC）用于热循环一次性微流控卡的放大室。

公开(公告)号：US07608399B2

公开(公告)日：2009-10-27

申请号：US11768076

申请日：2007-06-25

申请人： Michael W. Reed ,  Oliver Z. Nanassy ,  Paul V. Haydock ,  Nigel Rudra Sharma ,  Ronald L. Bardell ,  Perry Hargrave

发明人： Michael W. Reed ,  Oliver Z. Nanassy ,  Paul V. Haydock ,  Nigel Rudra Sharma ,  Ronald L. Bardell ,  Perry Hargrave

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  B01L3/5027 ,  B01L3/50273 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0457 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/686 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/137 ,  C12Q2563/173

摘要： Device and methods for extracting and analyzing nucleic acids from biological samples.

摘要翻译： 从生物样品中提取和分析核酸的装置和方法。

公开(公告)号：US07223371B2

公开(公告)日：2007-05-29

申请号：US10370433

申请日：2003-02-20

申请人： Jon W. Hayenga ,  Bernhard H. Weigl ,  Ronald L. Bardell ,  Christopher J. Morris

发明人： Jon W. Hayenga ,  Bernhard H. Weigl ,  Ronald L. Bardell ,  Christopher J. Morris

IPC分类号： B01L3/02 ,  B01L11/00 ,  B32B5/02 ,  B32B27/04 ,  B32B27/12

CPC分类号： G01N15/1404 ,  B01L2200/0636 ,  B01L2300/0627 ,  B01L2300/0861 ,  B01L2300/0864 ,  G01N2015/149 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Described herein is microfluidic device for joining fluids and a related method for doing the same. The device according to the present invention includes a microfluidic junction, an outlet channel, and a plurality of circuit units. A microfluidic junction is an area for converging multiple fluids. An outlet channel is capable of receiving fluid from the microfluidic junction. An outlet channel includes a first end connected with the microfluidic junction, a second end connected with a waste reservoir, and an analysis region positioned between the first end and the second end of the outlet channel. The device also includes a plurality of circuit units. Each circuit unit includes a source channel with a first end capable of receiving sample fluid and a second end connected with the microfluidic junction; a branch channel connected with the source channel at an intersection; and a flow diversion system capable of differentially directing fluid flowing through a source channel either into the microfluidic junction or into a branch channel.

摘要翻译： 这里描述的是用于接合流体的微流体装置和用于进行流体的相关方法。 根据本发明的装置包括微流体结，出口通道和多个电路单元。 微流体结是用于会聚多种流体的区域。 出口通道能够从微流体结接收流体。 出口通道包括与微流体结连接的第一端，与废物储存器连接的第二端和位于出口通道的第一端和第二端之间的分析区域。 该装置还包括多个电路单元。 每个电路单元包括源通道，其具有能够接收样品流体的第一端和与微流体结连接的第二端; 在交叉口与源通道连接的分支通道; 以及能够将流过源通道的流体差分地引导到微流体结或分支通道中的流动分流系统。