公开(公告)号：US09080941B2

公开(公告)日：2015-07-14

申请号：US14277503

申请日：2014-05-14

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Kashan Ali Shaikh ,  Mengli Wang ,  Adriana Ines Larriera Moreno ,  Jessica Godin Karp ,  Christine Lynne Pitner

IPC: B01L99/00 ,  G01N21/05 ,  C12Q1/68 ,  B01L3/00 ,  B01L9/00 ,  B32B37/00 ,  B32B37/18 ,  B32B38/00

CPC classification number: G01N21/05 ,  B01L3/5027 ,  B01L3/502707 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0655 ,  B32B37/0076 ,  B32B37/182 ,  B32B38/0008 ,  B32B2535/00 ,  C12Q1/6841 ,  G01N2021/058

Abstract: A microfluidic flow cell subassembly, which may be assembled into a flow cell having fluidic connections outside of the main substrate, is described for encapsulating a sample to allow for subsequent controlled delivery of reagents to the sample, such as multiplexed in situ biomarker staining and analysis. As configured, the subassembly comprises a substrate layer forms a flexible optically transparent lid which is capable of bending in either direction to alter the internal dimensions of the subassembly. Methods of use are also disclosed.

Abstract translation: 描述了可以组装成具有在主底物外部的流体连接的流动池的微流体流动池子组件，用于封装样品以允许随后控制的试剂递送到样品中，例如原位复合生物标记染色和分析 。 如所配置的，子组件包括基底层，形成柔性的光学透明盖，该盖能够沿任一方向弯曲以改变子组件的内部尺寸。 还公开了使用方法。

公开(公告)号：US20140248617A1

公开(公告)日：2014-09-04

申请号：US14277503

申请日：2014-05-14

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Kashan Ali Shaikh ,  Mengli Wang ,  Adriana Ines Larriera Moreno ,  Jessica Godin Karp ,  Christine Lynne Pitner

IPC: G01N21/05 ,  C12Q1/68

CPC classification number: G01N21/05 ,  B01L3/5027 ,  B01L3/502707 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0655 ,  B32B37/0076 ,  B32B37/182 ,  B32B38/0008 ,  B32B2535/00 ,  C12Q1/6841 ,  G01N2021/058

Abstract: A microfluidic flow cell subassembly, which may be assembled into a flow cell having fluidic connections outside of the main substrate, is described for encapsulating a sample to allow for subsequent controlled delivery of reagents to the sample, such as multiplexed in situ biomarker staining and analysis. As configured, the subassembly comprises a substrate layer forms a flexible optically transparent lid which is capable of bending in either direction to alter the internal dimensions of the subassembly. Methods of use are also disclosed.

Abstract translation: 描述了可以组装成具有在主底物外部的流体连接的流动池的微流体流动池子组件，用于封装样品以允许随后控制的试剂递送到样品中，例如原位复合生物标记染色和分析 。 如所配置的，子组件包括基底层，形成柔性的光学透明盖，该盖能够沿任一方向弯曲以改变子组件的内部尺寸。 还公开了使用方法。