公开(公告)号：US20170109880A1

公开(公告)日：2017-04-20

申请号：US15270061

申请日：2016-09-20

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Yousef Al-Kofahi ,  Anup Sood ,  Fiona Ginty ,  Qing Li ,  Christopher James Sevinsky ,  Alberto Santamaria-Pang

IPC: G06T7/00

CPC classification number: G06T7/0012 ,  G06T7/12 ,  G06T7/143 ,  G06T7/174 ,  G06T7/181 ,  G06T7/194 ,  G06T11/008 ,  G06T11/60 ,  G06T2207/10004 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/20076 ,  G06T2207/20081 ,  G06T2207/30024 ,  G06T2207/30101 ,  G06T2207/30172 ,  G06T2207/30204 ,  G06T2211/404

Abstract: Disclosed are novel computer-implemented methods for creating a blood vessel map of a biological tissue. The methods comprise the steps of, accessing image data corresponding to multi-channel multiplexed image of a fluorescently stained biological tissue manifesting expression levels of a primary marker and at least one auxiliary marker of blood vasculature, and extracting features of blood vessels using the primary marker as an input to create a single channel segmentation of the blood vessels. The method further comprises the steps of extracting features of blood vessels using the auxiliary marker to create auxiliary channels as a second input and apply multi-channel blood vessel enhancement. Blood vessel maps are created using the features and tracing the blood vasculature by iteratively extending the centerlines of the initial segmentation using statistical models and geometric rules

公开(公告)号：US20150285720A1

公开(公告)日：2015-10-08

申请号：US14247353

申请日：2014-04-08

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Bruce Allan Hay ,  Brian James Grimmond ,  Lisa Anne Lowery ,  Qing Li ,  Patrick McCoy Spooner ,  Christine Angela Morton

IPC: G01N1/42 ,  C12Q1/68 ,  G01N33/483

CPC classification number: C12Q1/6806 ,  G01N33/4833 ,  C12Q2527/101 ,  C12Q2527/125

Abstract: Disclosed are novel cold fixation methods and reagents comprising contacting a biological sample with a reagent for fixation at a temperature of less than 20° C. where the reagent comprises an aqueous buffer, a water soluble alkylnitrile, C2 to C6 alkyl ester, or combination thereof, and formaldehyde to the aqueous buffer solution and removing the biological sample from contact with the reagent.

Abstract translation: 公开了新颖的冷固定方法和试剂，包括使生物样品与低于20℃的温度的试剂接触，其中试剂包含水性缓冲液，水溶性烷基腈，C2至C6烷基酯或其组合 ，并将甲醛加入到缓冲溶液中，并将生物样品与试剂接触。