公开(公告)号：US11007212B2

公开(公告)日：2021-05-18

申请号：US16016963

申请日：2018-06-25

Applicant: General Electric Company

Inventor： Anup Sood ,  Lisa Anne Lowery ,  Praveena Mohan ,  James Edward Rothman ,  John Donald Burczak ,  Hae Won Lim

IPC: A61K47/64 ,  A61K31/713 ,  A61K47/54 ,  A61K47/69 ,  A61K41/00 ,  C12N15/87 ,  C12N15/11 ,  A61K9/00 ,  A61K47/06 ,  A61M37/00 ,  C12N15/113

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

公开(公告)号：US20190060347A1

公开(公告)日：2019-02-28

申请号：US16016963

申请日：2018-06-25

Applicant: General Electric Company

Inventor： Anup Sood ,  Lisa Anne Lowery ,  Praveena Mohan ,  James Edward Rothman ,  John Donald Burczak ,  Hae Won Lim

IPC: A61K31/713 ,  A61K41/00 ,  A61K47/64 ,  A61K47/54 ,  C12N15/113 ,  A61K47/06 ,  A61M37/00 ,  A61K9/00 ,  C12N15/11 ,  C12N15/87 ,  A61K47/69

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

公开(公告)号：US20180305718A1

公开(公告)日：2018-10-25

申请号：US15491125

申请日：2017-04-19

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Patrick McCoy Spooner ,  John Anthony Schiel ,  Lisa Anne Lowery ,  Anja Josifa Smith

IPC: C12N15/90 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/20

Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5′ flanking sequence and a donor 3′ flanking sequence. The donor 5′ flanking sequence and the donor 3′ flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.

公开(公告)号：US20170007636A1

公开(公告)日：2017-01-12

申请号：US15219419

申请日：2016-07-26

Applicant: General Electric Company

Inventor： Praveena Mohan ,  Anup Sood ,  James Edward Rothman ,  John Donald Burczak ,  Hae Won Lim ,  Lisa Anne Lowery

IPC: A61K31/713 ,  C12N15/113 ,  A61M37/00 ,  A61K47/48 ,  A61K47/06 ,  A61K41/00 ,  A61K9/00

CPC classification number: A61K31/713 ,  A61K9/0019 ,  A61K41/0028 ,  A61K47/06 ,  A61K47/554 ,  A61K47/64 ,  A61K47/643 ,  A61K47/6925 ,  A61M37/0092 ,  A61M2037/0007 ,  C12N15/111 ,  C12N15/1136 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/3515 ,  C12N2320/32

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Abstract translation: 本发明涉及一种微泡复合物，其包含具有外壳的微泡，所述微泡包含天然和包封全氟化碳气体的变性白蛋白的混合物，治疗剂，具有连接于治疗剂的一端的双功能连接体，另一端连接于配体 并且其中配体通过疏水相互作用结合到微泡的另一个壳体。 还包括用于将上述微泡复合物递送到组织靶的方法。

公开(公告)号：US10004760B2

公开(公告)日：2018-06-26

申请号：US15219419

申请日：2016-07-26

Applicant: General Electric Company

Inventor： Praveena Mohan ,  Anup Sood ,  James Edward Rothman ,  John Donald Burczak ,  Hae Won Lim ,  Lisa Anne Lowery

IPC: A61K31/713 ,  A61K41/00 ,  C12N15/87 ,  C12N15/11 ,  A61K9/00 ,  A61K47/06 ,  A61M37/00 ,  C12N15/113 ,  A61K47/54 ,  A61K47/64 ,  A61K47/69

CPC classification number: A61K31/713 ,  A61K9/0019 ,  A61K41/0028 ,  A61K47/06 ,  A61K47/554 ,  A61K47/64 ,  A61K47/643 ,  A61K47/6925 ,  A61M37/0092 ,  A61M2037/0007 ,  C12N15/111 ,  C12N15/1136 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/3515 ,  C12N2320/32

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

公开(公告)号：US20150285720A1

公开(公告)日：2015-10-08

申请号：US14247353

申请日：2014-04-08

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Bruce Allan Hay ,  Brian James Grimmond ,  Lisa Anne Lowery ,  Qing Li ,  Patrick McCoy Spooner ,  Christine Angela Morton

IPC: G01N1/42 ,  C12Q1/68 ,  G01N33/483

CPC classification number: C12Q1/6806 ,  G01N33/4833 ,  C12Q2527/101 ,  C12Q2527/125

Abstract: Disclosed are novel cold fixation methods and reagents comprising contacting a biological sample with a reagent for fixation at a temperature of less than 20° C. where the reagent comprises an aqueous buffer, a water soluble alkylnitrile, C2 to C6 alkyl ester, or combination thereof, and formaldehyde to the aqueous buffer solution and removing the biological sample from contact with the reagent.

Abstract translation: 公开了新颖的冷固定方法和试剂，包括使生物样品与低于20℃的温度的试剂接触，其中试剂包含水性缓冲液，水溶性烷基腈，C2至C6烷基酯或其组合 ，并将甲醛加入到缓冲溶液中，并将生物样品与试剂接触。