中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

97 records (took 0.029 seconds)

    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1,4-CYCLOHEXANEDIMETHANOL
    11.
    发明申请
    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1,4-CYCLOHEXANEDIMETHANOL 审中-公开
    用于生产1,4-环己烷二甲醇的微生物和方法

    公开(公告)号：US20140363864A1

    公开(公告)日：2014-12-11

    申请号：US14152799

    申请日：2014-01-10

    Applicant: Genomatica, Inc.

    Inventor： Priti Pharkya ,  Mark J. Burk

    IPC: C12P7/02 ,  C12N15/81 ,  C12N15/78 ,  C12N15/75 ,  C12N15/76 ,  C12N15/74 ,  C12N15/70

    CPC classification number: C12P7/02 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12N15/76 ,  C12N15/78 ,  C12N15/81 ,  C12N15/815 ,  C12P7/18

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4-cyclohexanedimethanol.

    Abstract translation: 非天然存在的微生物体包括具有1,4-环己烷二甲醇途径的微生物生物体，其包含至少一种编码1,4-环己烷二甲醇途径酶的外源核酸，其以足以产生1,4-环己烷二甲醇的量表达。 生产1,4-环己烷二甲醇的方法包括培养具有1,4-环己烷二甲醇途径的非天然存在的微生物。 该途径包括至少一种编码1,4-环己烷二甲醇途径酶的外源核酸，其在足以在足够的时间内产生1,4-环己烷二甲醇以足够量产生1,4-环己烷二甲醇。

    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
    14.
    发明授权
    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate 有权

    公开(公告)号：US11525149B2

    公开(公告)日：2022-12-13

    申请号：US15600479

    申请日：2017-05-19

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

    IPC: C12P7/46 ,  C12N15/00 ,  C12N9/00 ,  C12P7/04 ,  C12P7/40 ,  C12N9/88

    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors
    16.
    发明授权
    Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors 有权

    公开(公告)号：US11371046B2

    公开(公告)日：2022-06-28

    申请号：US15270578

    申请日：2016-09-20

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    IPC: C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12P7/52 ,  C12P17/04 ,  B01D3/00 ,  C12N15/70 ,  C12N15/81

    Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

    Organisms for the production of 1,3-butanediol
    17.
    发明授权
    Organisms for the production of 1,3-butanediol 有权

    公开(公告)号：US11180780B2

    公开(公告)日：2021-11-23

    申请号：US15616841

    申请日：2017-06-07

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    IPC: C12N9/88 ,  C12N9/90 ,  C12P7/16 ,  C12N15/00 ,  C12P7/18 ,  C12N15/52 ,  C12N9/04 ,  C07C29/80 ,  C12N9/02 ,  C12N9/10

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO
    19.
    发明申请
    MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO 有权

    公开(公告)号：US20210123078A1

    公开(公告)日：2021-04-29

    申请号：US16894600

    申请日：2020-06-05

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    IPC: C12P7/18 ,  C12N15/52

    Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH
    20.
    发明申请
    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH 有权

    公开(公告)号：US20210002194A1

    公开(公告)日：2021-01-07

    申请号：US16848430

    申请日：2020-04-14

    Applicant: Genomatica, Inc.

    Inventor： Warren Clark ,  Michael Japs ,  Mark J. Burk

    IPC: C07C29/76 ,  C12P7/18

    Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.

Patent Agency Ranking