公开(公告)号：US11312741B2

公开(公告)日：2022-04-26

申请号：US16094508

申请日：2017-04-19

Applicant: GLYCOM A/S

Inventor： Pierre Chassagne ,  Nikolay Khanzhin ,  Martin Matwiejuk ,  Markus Jondelius Hederos ,  Niels Banke

IPC: C07H13/04 ,  B01D61/02 ,  B01D61/14 ,  B01D61/58 ,  C07H1/06 ,  C12P19/28 ,  B01D15/36 ,  B01J41/07 ,  B01D61/16 ,  B01J39/05 ,  B01D15/18 ,  B01J20/20 ,  G01N30/96 ,  C12P19/26 ,  G01N30/02

Abstract: The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.

公开(公告)号：US10005807B2

公开(公告)日：2018-06-26

申请号：US14783783

申请日：2014-04-11

Applicant: GLYCOM A/S

Inventor： Pierre Chassagne ,  Nikolay Khanzhin ,  Markus Jondelius Hederos ,  Elise Champion ,  Martin Matwiejuk ,  Gyula Dekany

IPC: C07H5/04 ,  C07H1/00

CPC classification number: C07H5/04 ,  C07H1/00

Abstract: An achemo-enzymatic synthesis of oligosaccharides of formula 1 is presented wherein R is selected from —OH, —N 3 and —OR 6 wherein R 6 is selected from allyl optionally substituted by one or more methyl, propargyl optionally substituted by one or more methyl, 2-trimethylsilyl-ethyl, —(CH 2) n —NH 2 and —(CH 2) n —N 3 wherein integer n is to 10, preferably 2 or 3, R is selected from sialyl moiety, —SO 3 H and —CH(R)—COOH wherein R is selected from H, alkyl and benzyl, R 2 is selected from H and fucosyl, R 3 is selected from H and sialyl, R 4 is selected from H and fucosyl, provided that at least one of R 3 and R 4 is H, and A is a divalent carbohydrate linker, having important biological activities and significant commercial value for the pharmaceutical and food industry.

公开(公告)号：US20140235850A1

公开(公告)日：2014-08-21

申请号：US14348334

申请日：2012-10-01

Applicant: Glycom A/S

Inventor： Imre Kovács ,  István Bajza ,  Markus Hederos ,  Gyula Dekany ,  Sándor Demkó ,  Nikolay Khanzhin

IPC: C07H1/00 ,  C07H3/04 ,  C07H3/06

CPC classification number: C07H1/00 ,  A23L29/30 ,  C07H3/04 ,  C07H3/06 ,  C07H15/18

Abstract: The invention relates to a method for making precursors of HMO core structures comprising a step of reacting an N-acetyllactosamine or lacto-N-biose derivative donor with a lactose or N-acetyllactosamine derivative acceptor, wherein the donor is an oxazoline donor.

Abstract translation: 本发明涉及一种制备HMO核心结构的前体的方法，包括使N-乙酰基乳糖胺或乳-N-降糖衍生物供体与乳糖或N-乙酰基乳糖胺衍生物受体反应的步骤，其中供体是恶唑啉供体。