公开(公告)号：US11214588B2

公开(公告)日：2022-01-04

申请号：US16626245

申请日：2018-06-27

Applicant: GLYCOM A/S

Inventor： Nikolay Khanzhin ,  Markus Jondelius Hederos

IPC: A23L33/00 ,  C12P19/18 ,  C08B37/00 ,  B01D61/02 ,  B01D61/58 ,  B01D69/12 ,  C07H3/06 ,  C12P19/04 ,  B01D61/14 ,  C07H1/06 ,  B01D61/04 ,  B01D69/02 ,  B01D71/56 ,  B01D71/58 ,  C12P19/12

Abstract: This invention relates to a method for the enzymatic synthesis of oligosaccharides, preferably human milk oligosaccharides (HMOs) The method comprises the enzymatic transfer of a glycosyl moiety and subsequent removal of by-products, such as lactose, by nanofiltration using a membrane comprising an active polyamide layer.

公开(公告)号：US10899782B2

公开(公告)日：2021-01-26

申请号：US16082848

申请日：2017-03-07

Applicant: GLYCOM A/S

Inventor： Martin Matwiejuk ,  Nikolay Khanzhin ,  Pierre Chassagne ,  Markus Jondelius Hederos

IPC: C07H1/08 ,  C07H1/06 ,  B01D61/14 ,  C08B37/00 ,  B01D61/16 ,  C07H13/04 ,  A23L33/195 ,  B01D15/36 ,  C07H23/00 ,  C12P19/04 ,  C12P19/18 ,  B01D61/02 ,  B01D61/58 ,  C07H13/02

Abstract: The present invention relates to a method for separating sialylated oligosaccharides from a fermentation broth in which they are produced by a genetically modified microorganism The separation comprises the steps of: i) ultrafiltration; ii) nano-filtration; iii) optionally, activated charcoal treatment; and iv) treatment with strong anion and/or cation exchange resin.

公开(公告)号：US12065462B2

公开(公告)日：2024-08-20

申请号：US16626217

申请日：2018-06-27

Applicant: GLYCOM A/S

Inventor： Nikolay Khanzhin

IPC: C07H1/06 ,  A23L33/00 ,  B01D61/02 ,  B01D61/04 ,  B01D61/14 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18

CPC classification number: C07H1/06 ,  A23L33/40 ,  B01D61/027 ,  B01D61/04 ,  B01D61/146 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/0003 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18 ,  A23L33/00 ,  A23V2002/00 ,  B01D61/145 ,  B01D2315/16 ,  B01D2325/20

Abstract: The invention relates to a method for separating sialylated oligosaccharides, preferably sialylated human milk oligosaccharides (HMOs), from disaccharides, preferably lactose, produced by a fermentation or enzymatic process.

公开(公告)号：US11419884B2

公开(公告)日：2022-08-23

申请号：US16312506

申请日：2017-06-23

Applicant: GLYCOM A/S

Inventor： Nikolay Khanzhin ,  Markus Jondelius Hederos ,  Louise Kristine Vigsnæs ,  Bruce McConnell

IPC: A61K31/702 ,  C12P1/04 ,  A61P31/04 ,  A61K31/7016 ,  C07H1/00 ,  C12P19/04 ,  C12N9/10 ,  A61K31/716 ,  A61K31/7032

Abstract: The present disclosure relates to synthetic mixtures of certain human milk oligosaccharides and uses thereof. Particular mixtures provided herein include: lacto-N-triose II, lacto-N-neotetraose, para-lacto-N-neohexaose, and, optionally, lactose; and lacto-N-triose II, lacto-N-tetraose, para-lacto-N-hexaose II, and, optionally, lactose. These mixtures are useful in the prevention and treatment of viral and bacterial infections.

公开(公告)号：US11142541B2

公开(公告)日：2021-10-12

申请号：US16626122

申请日：2018-06-27

Applicant: GLYCOM A/S

Inventor： Nikolay Khanzhin ,  Markus Jondelius Hederos

IPC: C07H1/06 ,  B01D61/02 ,  B01D61/04 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C12P19/04 ,  C08B37/00 ,  C12P19/12 ,  A23L33/00 ,  C12P19/18

Abstract: The invention relates to separation of disaccharides from tri- or higher oligosaccharides by nanofiltration.

公开(公告)号：US10407516B2

公开(公告)日：2019-09-10

申请号：US15799386

申请日：2017-10-31

Applicant: Glycom A/S

Inventor： Pierre Chassagne ,  Nikolay Khanzhin ,  Agnese Maggi

IPC: C08B37/00 ,  A23L33/125 ,  A23L33/00

Abstract: Crystalline pLNnH, useful in a pharmaceutical or nutritional composition, is disclosed.

公开(公告)号：US20150320780A1

公开(公告)日：2015-11-12

申请号：US14651710

申请日：2013-12-13

Applicant: GLYCOM A/S

Inventor： Elise Champion ,  Gyula Dekany ,  Nikolay Khanzhin ,  Pierre Chassagne ,  Györgyi Osztrovszky

IPC: A61K31/7016 ,  A23L1/09 ,  A23L1/29 ,  C12P19/14 ,  C12P19/12

CPC classification number: A61K31/7016 ,  A23L5/00 ,  A23L7/00 ,  A23L29/30 ,  A23L33/10 ,  A23L33/125 ,  A23L33/40 ,  A23V2002/00 ,  A61K31/702 ,  C07H1/00 ,  C07H3/06 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14

Abstract: A method to form a mixture containing at least one of 2′-FL (2′-O-fucosyllactose, Fuc(α1-2)Gal(β1-4)Glc) and 3-FL (3-O-fucosyllactose, Gal(β1-4)[Fuc(α1-3)]Glc) characterized in that DFL (difucosyl-lactose, Fuc(α1-2)Gal(β1-4)[Fuc(α1-3)]Glc) is subjected to partial hydrolysis, e.g. enzymatic hydrolysis or acid hydrolysis.

Abstract translation: 形成含有2'-FL（2'-O-岩藻糖基乳糖，Fuc（α1-2）Gal（＆bgr; 1-4）Glc）和3-FL（3-O-岩藻糖基乳糖）中至少一种的混合物的方法， Gal（＆bgr; 1-4）[Fuc（α1-3）] Glc），其特征在于DFL（二氟蔗糖 - 乳糖，Fuc（α1-2）Gal（＆bgr; 1-4）[Fuc（α1-3）] Glc ）进行部分水解，例如 酶水解或酸水解。

公开(公告)号：US20140235840A1

公开(公告)日：2014-08-21

申请号：US14348432

申请日：2012-09-28

Applicant: GLYCOM A/S

Inventor： Nikolay Khanzhin ,  Julien Boutet ,  Filippo Bonaccorsi ,  Gyula Dekany

IPC: C07H1/00 ,  C07H3/02 ,  C07H9/04

CPC classification number: C07H1/00 ,  C07D493/04 ,  C07H3/02 ,  C07H3/08 ,  C07H9/02 ,  C07H9/04 ,  C07H11/00

Abstract: A process for converting D-glucose into L-fucose, where a first aspect of the disclosure relates to a method of making a compound of formula (1) wherein R is independently H, alkyl or phenyl or, preferably, wherein the two germinal R groups together with the carbon atom to which they are attached form a C3-s cycloalkylidene group, including the step of treating a compound of formula (2) wherein R is defined above and R1 is a sulphonate leaving group, with a reducing complex metal hydride and, preferably, a base to form the compound of formula (1); a compound of formula (13).

Abstract translation: 将D-葡萄糖转化为L-岩藻糖的方法，其中本公开的第一方面涉及制备式（1）化合物的方法，其中R独立地为H，烷基或苯基，或优选地，其中两个生发R 基团与它们所连接的碳原子一起形成C 3 -S亚环烷基，包括用还原性络合物金属氢化物处理式（2）化合物，其中R定义如上，R1是磺酸根离去基团的步骤 和优选地形成式（1）化合物的碱; 式（13）的化合物。

公开(公告)号：US11919919B2

公开(公告)日：2024-03-05

申请号：US17646093

申请日：2021-12-27

Applicant: GLYCOM A/S

Inventor： Nikolay Khanzhin ,  Markus Jondelius Hederos

IPC: C07H1/06 ,  A23L3/00 ,  A23L33/00 ,  B01D61/02 ,  B01D61/04 ,  B01D61/14 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18

CPC classification number: C07H1/06 ,  A23L33/40 ,  B01D61/027 ,  B01D61/04 ,  B01D61/146 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/0003 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18 ,  A23L33/00 ,  A23V2002/00 ,  B01D61/145 ,  B01D2315/16 ,  B01D2325/20

Abstract: This invention relates to a method for the enzymatic synthesis of oligosaccharides, preferably human milk oligosaccharides (HMOs) The method comprises the enzymatic transfer of a glycosyl moiety and subsequent removal of by-products, such as lactose, by nanofiltration using a membrane comprising an active polyamide layer.

公开(公告)号：US11384110B2

公开(公告)日：2022-07-12

申请号：US16346057

申请日：2017-10-31

Applicant: Glycom A/S

Inventor： Pierre Chassagne ,  Martin Matwiejuk ,  Nikolay Khanzhin ,  Markus Jondelius Hederos

IPC: C07H3/06 ,  A61K31/702 ,  F26B5/04 ,  F26B21/14 ,  C07H13/04 ,  C07H1/00 ,  A23L33/21 ,  C07H1/06

Abstract: The present invention relates to a method of removing residual organic solvent from crystalline oligosaccharides by the use of water vapour as well as the pharmaceutically and nutritionally suitable crystalline oligosaccharides obtained by said method.