公开(公告)号：US20180057895A1

公开(公告)日：2018-03-01

申请号：US15618598

申请日：2017-06-09

Applicant: GEN-PROBE INCORPORATED

Inventor： Kui GAO ,  Jeffrey M. LINNEN ,  Kurt C. NORTON ,  Patricia C. GORDON ,  Dat DO ,  Tan N. LE

IPC: C12Q1/70

CPC classification number: C12Q1/706 ,  C12Q1/701 ,  C12Q2600/16 ,  Y02A50/54

Abstract: Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

公开(公告)号：US20240360435A1

公开(公告)日：2024-10-31

申请号：US18771684

申请日：2024-07-12

Applicant: Gen-Probe Incorporated

Inventor： Kui GAO ,  Jijumon CHELLISERRY ,  Jeffrey LINNEN

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q1/70 ,  Y02A50/30

Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

公开(公告)号：US20230357872A1

公开(公告)日：2023-11-09

申请号：US18355297

申请日：2023-07-19

Applicant: Gen-Probe Incorporated

Inventor： Kui GAO ,  Edgar O. ONG ,  Jennifer COLE ,  Jeffrey M. Linnen

IPC: C12Q1/70

CPC classification number: C12Q1/707

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

公开(公告)号：US20200308661A1

公开(公告)日：2020-10-01

申请号：US16869720

申请日：2020-05-08

Applicant: Gen-Probe Incorporated

Inventor： Jijumon CHELLISERRY ,  Kui GAO ,  Jeffrey M. LINNEN

IPC: C12Q1/6893 ,  C12N1/06 ,  G01N33/48 ,  C12Q1/6806

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

公开(公告)号：US20160201144A1

公开(公告)日：2016-07-14

申请号：US14918131

申请日：2015-10-20

Applicant: Gen-Probe Incorporated

Inventor： Jijumon CHELLISERRY ,  Kui GAO ,  Jeffrey M. LINNEN

IPC: C12Q1/68

CPC classification number: C12Q1/6893 ,  C12N1/06 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

Abstract translation: 本发明提供了用于裂解红细胞的裂解试剂，从而以适合于分析的形式从寄生生物体释放靶，例如RNA。 试剂至少包括氯化铵和阴离子洗涤剂，并且可以包括抗凝剂。 该试剂用于裂解红细胞，保护释放的靶免受裂解物中的降解，并且与目标捕获，扩增，检测或测序等目标分析的后续步骤兼容。

公开(公告)号：US20150232920A1

公开(公告)日：2015-08-20

申请号：US14565099

申请日：2014-12-09

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael M. BECKER ,  Kui GAO ,  Wai-Chung LAM

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2545/114 ,  C12Q2545/101 ,  C12Q2531/113

Abstract: Method of detecting methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) in a nucleic acid coamplification assay. The invention advantageously reduces the incidence of false-positive MRSA determinations in real-time assays by requiring satisfaction of a threshold criterion that excludes certain co-infections from the MRSA determination. The invention further provides for determination of MSSA, even when the MSSA is present in combination with methicillin-resistant coagulase-negative (MR-CoNS) bacteria at high or low levels.