公开(公告)号：US09222113B2

公开(公告)日：2015-12-29

申请号：US13936878

申请日：2013-07-08

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Wei Niu ,  John D. Trawick ,  Robert Haselbeck

IPC: C12P7/18 ,  C12P17/04 ,  C12N1/20

CPC classification number: C12P17/04 ,  C12N1/20 ,  C12N15/52 ,  C12P7/18

Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

Abstract translation: 本发明提供包含1,4-丁二醇（14-BDO）和γ-丁内酯（GBL）途径的非天然存在的微生物生物，其包含至少一种编码14-BDO和GBL途径酶的外源核酸，其以足够的量表达， 生产14-BDO和GBL。 本发明另外提供了使用这种微生物生物来生产14-BDO和GBL的方法。

公开(公告)号：US11085015B2

公开(公告)日：2021-08-10

申请号：US15585078

申请日：2017-05-02

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Stephen J. Van Dien ,  Robin E. Osterhout ,  Mark J. Burk ,  John D. Trawick ,  Michael P. Kuchinskas ,  Brian Steer

IPC: C12P7/42 ,  C12P7/18 ,  C12N1/20 ,  C12N1/14 ,  C12N1/16 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C07C31/20 ,  C07C59/01 ,  C08G18/28 ,  C08G18/32 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.

公开(公告)号：US10550411B2

公开(公告)日：2020-02-04

申请号：US15889788

申请日：2018-02-06

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

IPC: C12P7/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12P19/32 ,  C12N15/70

Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

公开(公告)号：US20190264240A1

公开(公告)日：2019-08-29

申请号：US16216865

申请日：2018-12-11

Applicant: Genomatica, Inc.

Inventor： Robert Haselbeck ,  John D. Trawick ,  Wei Niu ,  Anthony P. Burgard

IPC: C12P7/18 ,  C12P7/42 ,  C12P7/24 ,  C12P13/00 ,  C12P19/40

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

公开(公告)号：US09109236B2

公开(公告)日：2015-08-18

申请号：US13920927

申请日：2013-06-18

Applicant: Genomatica, Inc.

Inventor： John D. Trawick ,  Mark J. Burk ,  Anthony P. Burgard

IPC: C12P7/18 ,  C12P7/04 ,  C12P7/42 ,  C12N1/20 ,  C12N1/00 ,  C07C29/00 ,  C07C27/26 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/42

Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.

Abstract translation: 具有异丙醇，4-羟基丁酸酯或1,4-丁二醇途径的非天然存在的微生物包括至少一种编码以足够量表达的异丙醇，4-羟基丁酸酯或1,4-丁二醇途径酶的外源核酸 产生异丙醇，4-羟基丁酸酯或1,4-丁二醇。 培养上述生物体以产生异丙醇，4-羟基丁酸酯或1,4-丁二醇。

公开(公告)号：US20140147900A1

公开(公告)日：2014-05-29

申请号：US13920927

申请日：2013-06-18

Applicant: Genomatica, Inc.

Inventor： John D. Trawick ,  Mark J. Burk ,  Anthony P. Burgard

IPC: C12P7/18

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/42

Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.

Abstract translation: 具有异丙醇，4-羟基丁酸酯或1,4-丁二醇途径的非天然存在的微生物包括至少一种编码以足够量表达的异丙醇，4-羟基丁酸酯或1,4-丁二醇途径酶的外源核酸 产生异丙醇，4-羟基丁酸酯或1,4-丁二醇。 培养上述生物体以产生异丙醇，4-羟基丁酸酯或1,4-丁二醇。