公开(公告)号：US11325125B2

公开(公告)日：2022-05-10

申请号：US16076074

申请日：2017-04-23

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Chien-Hua Chen

IPC: B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N1/40

Abstract: A fluid entrained particle separator may include an inlet passage to direct particles entrained in a fluid, a first separation passage branching from the inlet passage, a second separation passage branching from the inlet passage and electrodes to create electric field exerting a dielectrophoretic force on the particles to direct the particles to the first separation passage or the second separation passage, wherein the first separation passage, the second separation passage, the electric field and the dielectrophoretic force extend in a plane.

公开(公告)号：US20220118450A1

公开(公告)日：2022-04-21

申请号：US17417500

申请日：2019-04-30

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: B01L3/00 ,  B01L7/00

Abstract: The present disclosure relates to a microfluidic device including a microfluidic substrate and dry reagent-containing particles. The microfluidic substrate includes an ingress microfluidic channel that fluidly feeds an egress microfluidic channel through a microfluidic-retaining region that includes a microfluidic discontinuity feature, a particle-retaining chemical coating, or a combination thereof. The dry reagent-containing particles include a reagent that is releasable from the dry reagent-containing particles when exposed to a release fluid. The dry reagent-containing particles are retained within the microfluidic substrate at the microfluidic discontinuity feature or particle-retaining chemical coating in position to release the reagent into the egress microfluidic channel upon flow of release fluid from the ingress microfluidic channel through the microfluidic-retaining region.

公开(公告)号：US20220073863A1

公开(公告)日：2022-03-10

申请号：US17417339

申请日：2019-02-01

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov ,  Diane R. Hammerstad

IPC: C12M1/00 ,  C12M1/42 ,  C12M3/06

Abstract: In one example in accordance with the present disclosure, a chemical lysis system is described. The chemical lysis system includes a microfluidic channel to serially feed individual cells from a volume of cells to at least one chemical lysing device. Each chemical lysing device includes at least one lysing chamber to receive, from the microfluidic channel, a single cell to be lysed. The chemical lysing device also includes an orifice disposed in each lysing chamber to receive a lysing agent and a sensor to detect a state within the lysing chamber. A controller of the chemical lysis system analyzes a ruptured cell.

公开(公告)号：US11229912B2

公开(公告)日：2022-01-25

申请号：US16605706

申请日：2018-03-27

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov ,  Pavel Kornilovich ,  Erik D. Torniainen

IPC: B01L3/00 ,  G01N1/40

Abstract: An example system includes an input channel having a first end and a second end to receive particles through the first end, a separation chamber, at least two output channels, and an integrated pump to facilitate flow through the separation chamber. The separation chamber is in fluid communication with the second end of the input channel. The separation chamber has a passive separation structure, the passive separation structure including an array of columns spaced apart to facilitate separation of particles in a flow based on a size of the particles. Each output channel is in fluid communication with the separation chamber to receive separated particles. The integrated pump is positioned within at least one of the input channel or one of the at least two output channels.

公开(公告)号：US20210362116A1

公开(公告)日：2021-11-25

申请号：US16606368

申请日：2018-05-01

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Chien-Hua Chen

IPC: B01J13/02 ,  B01L3/00

Abstract: An example system includes a primary channel having a first end and a second end, at least two reagent reservoirs coupled to the first end, and a controller. Each reservoir contains a reagent in a fluid solution and is associated with an integrated pump to drive a reagent droplet from the corresponding reagent reservoir into the primary channel towards the second end. The controller is coupled to the integrated pumps and operates according to a sequence to actuate the integrated pumps, the sequence being indicative of reagents in the reagent reservoirs. The actuation of the pumps is to drive the reagent droplets from the reagent reservoirs into the primary channel in accordance with the sequence. The example system also includes a shell material reservoir with a shell material and an associated shell material pump to drive the shell material into the primary channel to encapsulate the reagent droplets.

公开(公告)号：US20210322990A1

公开(公告)日：2021-10-21

申请号：US16605780

申请日：2018-04-27

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Chien-Hua Chen

IPC: B01L7/00 ,  B01L3/00 ,  G01N33/543

Abstract: An example system includes an array of retaining features in a microfluidic cavity, an array of thermally controlled releasing features, and a controller coupled to each releasing feature in the array of releasing feature. Each retaining feature in the array of retaining features is to position capsules at a predetermined location, the capsules having a thermally degradable shell enclosing a biological reagent therein. Each releasing feature in the array of releasing features corresponds to a retaining feature and is to selectively cause degradation of the shell of a capsule. Each releasing feature is to generate thermal energy to facilitate degradation of the shell. The controller is to selectively activate at least one releasing feature in the array of thermally controlled releasing features to release the biological reagent in the capsules positioned at the retaining feature corresponding to the activated releasing feature.

公开(公告)号：US11141729B2

公开(公告)日：2021-10-12

申请号：US16605162

申请日：2018-01-24

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Alexander N. Govyadinov ,  Pavel Kornilovich

IPC: B01L3/00 ,  F04B19/00

Abstract: An object focuser may include a substrate, a sample fluid passage supported by the substrate, a first inertial pump supported by the substrate to pump a sample fluid entraining an object through the sample fluid passage, a first sheath fluid passage, a second inertial pump supported by the substrate to pump a first sheath fluid through the first sheath fluid passage, a second sheath fluid passage and a second inertial pump supported by the substrate to pump a second sheath fluid through the second sheath fluid passage. The first sheath fluid passage and the second sheath fluid passage are connected to the sample fluid passage at a convergence on opposite sides of the sample fluid passage.

公开(公告)号：US11131608B2

公开(公告)日：2021-09-28

申请号：US15756965

申请日：2015-10-23

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Anita Rogacs

IPC: G01N1/40 ,  G01N21/65 ,  B01D61/38

Abstract: A target analyte is extracted out of a sample fluid in a sample fluid passage by diffusing the target analyte through a supported liquid membrane to a product fluid passage. Extraction of the target analyte is accelerated by applying an electric field across and perpendicular to the supported liquid membrane with electrodes. Passage of selected ions across an exchange membrane extending between one of the electrodes and the supported liquid membrane is inhibited.

公开(公告)号：US20210239634A1

公开(公告)日：2021-08-05

申请号：US17049166

申请日：2018-08-09

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov

IPC: G01N27/02 ,  B01L3/00 ,  G01N1/28

Abstract: In one example in accordance with the present disclosure, a sample Sample Preparation System preparation system is described. The system includes a reaction chamber to hold at least one fluid. An inlet impedance sensor of the system detects a level of conductivity through at least one inlet of the reaction chamber and an outlet impedance sensor detects a level of conductivity through at least one outlet of the reaction chamber. A controller of the system, based on measured levels of conductivity, determines a state of a reaction within the reaction chamber.

公开(公告)号：US20210213449A1

公开(公告)日：2021-07-15

申请号：US17054499

申请日：2018-09-26

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Daixi Xin ,  Chien-Hua Chen

IPC: B01L3/00 ,  B01D57/02 ,  G01N1/28 ,  G01N33/49 ,  G01N35/08

Abstract: In example implementations, an apparatus is provided. The apparatus includes a microfluidic channel to receive a fluid containing a plurality of different cells. A dielectrophoresis (DEP) separator in the apparatus separates the plurality of different cells passing through DEP separator within the microfluidic channel. In addition, the apparatus includes a density separator to further separate a portion of the plurality of different cells from the DEP separator based on a density of each one of the plurality of different cells.